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Literature summary extracted from

  • Shibasaki, S.; Sakata, K.; Ishii, J.; Kondo, A.; Ueda, M.
    Development of a yeast protein fragment complementation assay (PCA) system using dihydrofolate reductase (DHFR) with specific additives (2008), Appl. Microbiol. Biotechnol., 80, 735-743.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.5.1.3 analysis development of a yeast protein fragment complementation assay system using dihydrofolate reductase and application for investigating eukaryotic protein-protein interaction in vivo. Fusion of human oncoprotein Ras and Ras-binding domain of Raf-1 to dihydrofolate reductase as a model and evaluation of interaction between these proteins Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.3 folic acid use in yeast protein fragment complementation assay system to prevent endogenous dihydrofolate reductase activity Saccharomyces cerevisiae
1.5.1.3 sulfanilamide use in yeast protein fragment complementation assay system to prevent endogenous dihydrofolate reductase activity Saccharomyces cerevisiae
1.5.1.3 trimethoprim use in yeast protein fragment complementation assay system to prevent endogenous dihydrofolate reductase activity Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.3 Saccharomyces cerevisiae
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