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Literature summary extracted from

  • Doukyu, N.; Shibata, K.; Ogino, H.; Sagermann, M.
    Purification and characterization of Chromobacterium sp. DS-1 cholesterol oxidase with thermal, organic solvent, and detergent tolerance (2008), Appl. Microbiol. Biotechnol., 80, 59-70.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.3.6 Zn2+ 1 mM, 34% loss of activity Chromobacterium sp. DS-1

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.6 0.026
-
 cholesterol
-
 Chromobacterium sp. DS-1

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.3.6 extracellular
-
 Chromobacterium sp. DS-1
-
-

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
1.1.3.6 Acetone 37°C, 24 h, 1 ml organic solvent added to 2 ml culture filtrate, 90% loss of activity Chromobacterium sp. DS-1
1.1.3.6 benzene 37°C, 24 h, 1 ml organic solvent added to 2 ml culture filtrate, stable Chromobacterium sp. DS-1
1.1.3.6 Butanol 37°C, 24 h, 1 ml organic solvent added to 2 ml culture filtrate, stable Chromobacterium sp. DS-1
1.1.3.6 chloroform 37°C, 24 h, 1 ml organic solvent added to 2 ml culture filtrate, stable Chromobacterium sp. DS-1
1.1.3.6 cyclooctane 37°C, 24 h, 1 ml organic solvent added to 2 ml culture filtrate, stable Chromobacterium sp. DS-1
1.1.3.6 dimethyl sulfoxide 37°C, 24 h, 1 ml organic solvent added to 2 ml culture filtrate, stable Chromobacterium sp. DS-1
1.1.3.6 Ethanol 37°C, 24 h, 1 ml organic solvent added to 2 ml culture filtrate, stable Chromobacterium sp. DS-1
1.1.3.6 Ethyl acetate 37°C, 24 h, 1 ml organic solvent added to 2 ml culture filtrate, stable Chromobacterium sp. DS-1
1.1.3.6 isopropanol 37°C, 24 h, 1 ml organic solvent added to 2 ml culture filtrate, stable Chromobacterium sp. DS-1
1.1.3.6 Methanol 37°C, 24 h, 1 ml organic solvent added to 2 ml culture filtrate, stable Chromobacterium sp. DS-1
1.1.3.6 p-xylene 37°C, 24 h, 1 ml organic solvent added to 2 ml culture filtrate, stable Chromobacterium sp. DS-1
1.1.3.6 toluene 37°C, 24 h, 1 ml organic solvent added to 2 ml culture filtrate, stable Chromobacterium sp. DS-1

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.6 Chromobacterium sp. DS-1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.6
-
 Chromobacterium sp. DS-1

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.3.6 culture supernatant
-
 Chromobacterium sp. DS-1
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.3.6 13.9
-
-
 Chromobacterium sp. DS-1

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.6 beta-cholestanol + O2 95% of the activity with cholesterol Chromobacterium sp. DS-1 ?
-
 ?
1.1.3.6 beta-sitosterol + O2 80% of the activity with cholesterol Chromobacterium sp. DS-1 ?
-
 ?
1.1.3.6 beta-stigmasterol + O2 36% of the activity with cholesterol Chromobacterium sp. DS-1 ?
-
 ?
1.1.3.6 cholesterol + O2
-
 Chromobacterium sp. DS-1 cholest-4-en-3-one + H2O2
-
 ?
1.1.3.6 dehydroepiandrosterone + O2 27% of the activity with cholesterol Chromobacterium sp. DS-1 ?
-
 ?
1.1.3.6 epiandrosterone + O2 15% of the activity with cholesterol Chromobacterium sp. DS-1 ?
-
 ?
1.1.3.6 ergosterol + O2 12% of the activity with cholesterol Chromobacterium sp. DS-1 ?
-
 ?
1.1.3.6 additional information no activity with epicholesterol Chromobacterium sp. DS-1 ?
-
 ?
1.1.3.6 pregnenolone + O2 95% of the activity with cholesterol Chromobacterium sp. DS-1 ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.3.6 65
-
-
 Chromobacterium sp. DS-1

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.3.6 40 80 40°C: about 70% of maximal activity, 80°C: about 40% of maximal activity Chromobacterium sp. DS-1

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.3.6 50
-
 240 min, enzyme retains 99% of its activity Chromobacterium sp. DS-1
1.1.3.6 60
-
 240 min, enzyme retains 87% of its activity Chromobacterium sp. DS-1
1.1.3.6 80
-
 60 min, the enzyme retains 90% of its activity Chromobacterium sp. DS-1
1.1.3.6 85
-
 30 min, 20% loss of activity Chromobacterium sp. DS-1
1.1.3.6 90
-
 30 min, 72% loss of activity Chromobacterium sp. DS-1

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.6 7
-
-
 Chromobacterium sp. DS-1

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.3.6 6 9.5 pH 6.0: about 65% of maximal activity, pH 9.5: about 60% of maximal activcity Chromobacterium sp. DS-1

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.3.6 3 11 30°C, 1 h, stable Chromobacterium sp. DS-1

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.6 FAD enzyme contains 1 mol of FAD per mole of protein Chromobacterium sp. DS-1