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Literature summary extracted from

  • Lee, S.H.; Park, S.J.; Lee, S.Y.; Hong, S.H.
    Biosynthesis of enantiopure (S)-3-hydroxybutyric acid in metabolically engineered Escherichia coli (2008), Appl. Microbiol. Biotechnol., 79, 633-641.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.2.4 subcloning in Escherichia coli strain XL1-Blue, and expression in Escherichia coli strain BL21 (DE3) under control of the T7 promoter, co-expression with the beta-ketothiolase gene from Ralstonia eutropha strain H16 and the (S)-3-hydroxybutyryl-CoA dehydrogenase gene from Ralstonia eutropha strain H16, or Clostridium acetobutylicum strain ATCC824 leads to biosynthesis of enantiomerically pure (S)-3-hydroxybutyric acid in the engineered Escherichia coli cells, extracellular product, method optimization, overview Bacillus cereus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.2.4 3-hydroxy-2-methylpropanoyl-CoA + H2O Bacillus cereus
-
 CoA + 3-hydroxy-2-methylpropanoate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.2.4 Bacillus cereus Q81DR3 strain ATCC 14579
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.2.4 0.073
-
 recombinant BCH in Escherichia coli strain BL21(DE3) crude extract Bacillus cereus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.2.4 3-hydroxy-2-methylpropanoyl-CoA + H2O
-
 Bacillus cereus CoA + 3-hydroxy-2-methylpropanoate
-
 ?
3.1.2.4 3-hydroxy-2-methylpropanoyl-CoA + H2O
-
 Bacillus cereus CoA + 3-hydroxy-2-methylpropanoate (S)-3-hydroxyacids and their esters, such as (S)-3-hydroxybutyric acid, can be used as chiral building blocks for the chemical synthesis of optically active fine chemicals such as antibiotics, vitamins, perfumes, and pheromones ?

Synonyms

EC Number Synonyms Comment Organism
3.1.2.4 3-hydroxyisobutyryl-CoA hydrolase
-
 Bacillus cereus
3.1.2.4 BCH
-
 Bacillus cereus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.2.4 37
-
 assay at Bacillus cereus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.2.4 7
-
 assay at Bacillus cereus