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Literature summary extracted from

  • Koschorreck, K.; Richter, S.M.; Ene, A.B.; Roduner, E.; Schmid, R.D.; Urlacher, V.B.
    Cloning and characterization of a new laccase from Bacillus licheniformis catalyzing dimerization of phenolic acids (2008), Appl. Microbiol. Biotechnol., 79, 217-224.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.10.3.2 expressed in Escherichia coli Bacillus licheniformis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.2 0.0043
-
 syringaldazine pH 7.0 Bacillus licheniformis
1.10.3.2 0.0065
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) pH 4.0 Bacillus licheniformis
1.10.3.2 0.0567
-
 2,6-dimethoxyphenol pH 7.0 Bacillus licheniformis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.10.3.2 65000
-
 x * 65000, SDS-PAGE Bacillus licheniformis

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.2 Bacillus licheniformis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.2
-
 Bacillus licheniformis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.2 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) + O2
-
 Bacillus licheniformis ?
-
 ?
1.10.3.2 2,6-dimethoxyphenol + O2
-
 Bacillus licheniformis ?
-
 ?
1.10.3.2 caffeic acid + O2
-
 Bacillus licheniformis ?
-
 ?
1.10.3.2 ferulic acid + O2
-
 Bacillus licheniformis ?
-
 ?
1.10.3.2 additional information CotA fails to oxidize coumaric acid, cinnamic acid, and vanillic acid Bacillus licheniformis ?
-
 ?
1.10.3.2 sinapic acid + O2
-
 Bacillus licheniformis ?
-
 ?
1.10.3.2 syringaldazine + O2
-
 Bacillus licheniformis ?
-
 ?
1.10.3.2 syringic acid + O2
-
 Bacillus licheniformis 2,6-dimethoxy-1,4-benzoquinone + CO2 + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.10.3.2 ? x * 65000, SDS-PAGE Bacillus licheniformis

Synonyms

EC Number Synonyms Comment Organism
1.10.3.2 CotA
-
 Bacillus licheniformis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.3.2 85
-
 oxidation of 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) Bacillus licheniformis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.10.3.2 70
-
 1 h, 57% loss of activity Bacillus licheniformis
1.10.3.2 80
-
 1 h, 92% loss of activity Bacillus licheniformis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.10.3.2 2 8 2,6-dimethoxyphenol pH 7.0 Bacillus licheniformis
1.10.3.2 83
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) pH 4.0 Bacillus licheniformis
1.10.3.2 100
-
 syringaldazine pH 7.0 Bacillus licheniformis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.2 4.2
-
 oxidation of 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) Bacillus licheniformis
1.10.3.2 7
-
 oxidation of syringaldazine or 2,6-dimethoxyphenol Bacillus licheniformis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.10.3.2 Bacillus licheniformis isoelectric focusing
-
 7