BRENDA - Enzyme Database show

Deletion of iscR stimulates recombinant clostridial Fe-Fe hydrogenase activity and H2-accumulation in Escherichia coli BL21(DE3)

Akhtar, M.K.; Jones, P.R.; Appl. Microbiol. Biotechnol. 78, 853-862 (2008)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.12.7.2
expressed in Escherichia coli BL21(DE3) cells
Clostridium acetobutylicum
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.12.7.2
Iron
one mol of HydA contains 7 mol iron
Clostridium acetobutylicum
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.12.7.2
Clostridium acetobutylicum
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.12.7.2
Ni-Sepharose 6 Fast Flow chromatography
Clostridium acetobutylicum
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.12.7.2
28
-
recombinant, purified enzyme
Clostridium acetobutylicum
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.12.7.2
H2 + oxidized ferredoxin
under anoxic conditions
684643
Clostridium acetobutylicum
H+ + reduced ferredoxin
-
-
-
r
1.12.7.2
H2 + oxidized methyl viologen
-
684643
Clostridium acetobutylicum
H+ + reduced methyl viologen
-
-
-
r
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.12.7.2
Ferredoxin
dependent
Clostridium acetobutylicum
1.12.7.2
iron-sulfur centre
-
Clostridium acetobutylicum
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.12.7.2
expressed in Escherichia coli BL21(DE3) cells
Clostridium acetobutylicum
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.12.7.2
Ferredoxin
dependent
Clostridium acetobutylicum
1.12.7.2
iron-sulfur centre
-
Clostridium acetobutylicum
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.12.7.2
Iron
one mol of HydA contains 7 mol iron
Clostridium acetobutylicum
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.12.7.2
Ni-Sepharose 6 Fast Flow chromatography
Clostridium acetobutylicum
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.12.7.2
28
-
recombinant, purified enzyme
Clostridium acetobutylicum
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.12.7.2
H2 + oxidized ferredoxin
under anoxic conditions
684643
Clostridium acetobutylicum
H+ + reduced ferredoxin
-
-
-
r
1.12.7.2
H2 + oxidized methyl viologen
-
684643
Clostridium acetobutylicum
H+ + reduced methyl viologen
-
-
-
r