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Literature summary extracted from
- Identification of galacto-N-biose phosphorylase from Clostridium perfringens ATCC13124 (2008), Appl. Microbiol. Biotechnol., 78, 465-471.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 2.4.1.211 | expression in Escherichia coli | Clostridium perfringens ATCC 13124 |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 2.4.1.211 | 0.43 | - |
phosphate | beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine as substrate | Bifidobacterium longum subsp. Longum |  |
| 2.4.1.211 | 0.52 | - |
phosphate | beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosmine as substrate | Clostridium perfringens ATCC 13124 | |
| 2.4.1.211 | 0.58 | - |
phosphate | beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosmine as substrate | Bifidobacterium longum subsp. Longum | |
| 2.4.1.211 | 0.66 | - |
phosphate | beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine as substrate | Clostridium perfringens ATCC 13124 | |
| 2.4.1.211 | 1.7 | - |
beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosmine | sequential bi-bi mechanism | Clostridium perfringens ATCC 13124 | |
| 2.4.1.211 | 2.1 | - |
beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine | - |
Bifidobacterium longum subsp. Longum | |
| 2.4.1.211 | 5.6 | - |
beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosmine | - |
Bifidobacterium longum subsp. Longum | |
| 2.4.1.211 | 21 | - |
beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine | - |
Clostridium perfringens ATCC 13124 | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.211 | 80000 | - |
SDS-PAGE | Clostridium perfringens ATCC 13124 |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 2.4.1.211 | Bifidobacterium longum subsp. Longum | - |
- |
- |
| 2.4.1.211 | Bifidobacterium longum subsp. Longum JCM 1217 | - |
- |
- |
| 2.4.1.211 | Clostridium perfringens ATCC 13124 | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 2.4.1.211 | of the recombinant protein by Ni-nitrilotriacetic acid agarose column chromatography | Clostridium perfringens ATCC 13124 |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.4.1.211 | beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosmine + phosphate | no activity with D-glucose, D-glucosamine, D-fructose, 2-deoxy-D-glucose, D-mannose, maltose, and sucrose as substrates | Clostridium perfringens ATCC 13124 | alpha-D-galactose 1-phosphate + N-acetyl-D-galactosamine | - |
? | |
| 2.4.1.211 | beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosmine + phosphate | - |
Bifidobacterium longum subsp. Longum | alpha-D-galactose 1-phosphate + N-acetyl-galactosamine | - |
? | |
| 2.4.1.211 | beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosmine + phosphate | - |
Bifidobacterium longum subsp. Longum JCM 1217 | alpha-D-galactose 1-phosphate + N-acetyl-galactosamine | - |
? | |
| 2.4.1.211 | beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine + phosphate | - |
Clostridium perfringens ATCC 13124 | alpha-D-galactose 1-phosphate + N-acetyl-D-glucosamine | - |
? | |
| 2.4.1.211 | beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine + phosphate | - |
Bifidobacterium longum subsp. Longum | alpha-D-galactose 1-phosphate + N-acetyl-glucosamine | - |
? | |
| 2.4.1.211 | beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine + phosphate | - |
Bifidobacterium longum subsp. Longum JCM 1217 | alpha-D-galactose 1-phosphate + N-acetyl-glucosamine | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 2.4.1.211 | galacto-N-biose phosphorylase | - |
Bifidobacterium longum subsp. Longum |
| 2.4.1.211 | galacto-N-biose phosphorylase | - |
Clostridium perfringens ATCC 13124 |
| 2.4.1.211 | LNBP | - |
Bifidobacterium longum subsp. Longum |
| 2.4.1.211 | LNBP | - |
Clostridium perfringens ATCC 13124 |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.211 | 45 | - |
- |
Clostridium perfringens ATCC 13124 |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.211 | 20 | 50 | in 100 mM buffer containing 20 micromolar DTT | Clostridium perfringens ATCC 13124 |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.211 | 6.5 | - |
- |
Clostridium perfringens ATCC 13124 |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.211 | 4.5 | 5.5 | in presence of sodium acetate | Clostridium perfringens ATCC 13124 |
| 2.4.1.211 | 5.5 | 6.5 | in presence of MES-NaOH | Clostridium perfringens ATCC 13124 |
| 2.4.1.211 | 6.5 | 7.5 | in presence of Mops-NaOH | Clostridium perfringens ATCC 13124 |
| 2.4.1.211 | 7.5 | 9 | in presence of Tris-HCl | Clostridium perfringens ATCC 13124 |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.211 | 4 | 6 | in presence of sodium acetate | Clostridium perfringens ATCC 13124 |
| 2.4.1.211 | 6 | 7 | in presence of MES-NaOH | Clostridium perfringens ATCC 13124 |
| 2.4.1.211 | 6.5 | 7.5 | in presence of Mops-NaOH | Clostridium perfringens ATCC 13124 |
| 2.4.1.211 | 7.5 | 9 | in presence of Tris-HCl | Clostridium perfringens ATCC 13124 |
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