EC Number | Cloned (Comment) | Organism |
---|---|---|
1.4.3.10 | expressed in Escherichia coli Top10 cells | Rhodococcus erythropolis |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.4.3.10 | E324A | the mutant enzyme shows activity with monoamines, which are not accepted by the wild type enzyme | Rhodococcus erythropolis |
1.4.3.10 | E324L | the mutant enzyme shows activity with monoamines, which are not accepted by the wild type enzyme | Rhodococcus erythropolis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.4.3.10 | (2-aminoethyl)-trimethylammonium | competitive inhibitor | Rhodococcus erythropolis | |
1.4.3.10 | 1,3-diaminopropane | competitive inhibitor | Rhodococcus erythropolis | |
1.4.3.10 | Aminoethanol | competitive inhibitor | Rhodococcus erythropolis | |
1.4.3.10 | ethylenediamine | competitive inhibitor | Rhodococcus erythropolis | |
1.4.3.10 | additional information | short diamines and monoamines strongly inhibit activity | Rhodococcus erythropolis | |
1.4.3.10 | n-butylamine | competitive inhibitor | Rhodococcus erythropolis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.4.3.10 | 0.0035 | - |
putrescine | wild type enzyme, in 50 mM Tris-HCl buffer, pH 9.0, at 25°C | Rhodococcus erythropolis | |
1.4.3.10 | 0.0082 | - |
putrescine | wild type enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 25°C | Rhodococcus erythropolis | |
1.4.3.10 | 0.018 | - |
cadaverine | wild type enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 25°C | Rhodococcus erythropolis | |
1.4.3.10 | 0.065 | - |
2-hydroxyputrescine | wild type enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 25°C | Rhodococcus erythropolis | |
1.4.3.10 | 0.1 | - |
spermidine | wild type enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 25°C | Rhodococcus erythropolis | |
1.4.3.10 | 0.17 | - |
1,6-diaminohexane | wild type enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 25°C | Rhodococcus erythropolis | |
1.4.3.10 | 0.33 | - |
L-ornithine | wild type enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 25°C | Rhodococcus erythropolis | |
1.4.3.10 | 0.47 | - |
spermine | wild type enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 25°C | Rhodococcus erythropolis | |
1.4.3.10 | 110 | - |
putrescine | mutant enzyme E324A, in 50 mM Tris-HCl buffer, pH 9.0, at 25°C | Rhodococcus erythropolis | |
1.4.3.10 | 110 | - |
putrescine | mutant enzyme E324L, in 50 mM Tris-HCl buffer, pH 9.0, at 25°C | Rhodococcus erythropolis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.4.3.10 | 54000 | - |
2 * 54000, SDS-PAGE | Rhodococcus erythropolis |
1.4.3.10 | 98750 | - |
theoretical molecular mass | Rhodococcus erythropolis |
1.4.3.10 | 108000 | - |
SDS-PAGE | Rhodococcus erythropolis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.4.3.10 | Rhodococcus erythropolis | B0F9F6 | fragment | - |
1.4.3.10 | Rhodococcus erythropolis NCIMB 11540 | B0F9F6 | fragment | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.4.3.10 | Q-Sepharose column chromatography | Rhodococcus erythropolis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.4.3.10 | 1,6-diaminohexane + O2 + H2O | 1.4% activity compared to putrescine | Rhodococcus erythropolis | 6-aminohexanal + NH3 + H2O2 | - |
? | |
1.4.3.10 | 2-hydroxyputrescine + O2 + H2O | 28% activity compared to putrescine | Rhodococcus erythropolis | ? | - |
? | |
1.4.3.10 | 4-amino-1-butanol + O2 + H2O | 0.6% activity compared to putrescine | Rhodococcus erythropolis | 4-hydroxybutanal + NH3 + H2O2 | - |
? | |
1.4.3.10 | cadaverine + O2 + H2O | 14% activity compared to putrescine | Rhodococcus erythropolis | 5-aminopentanal + NH3 + H2O2 | - |
? | |
1.4.3.10 | cadaverine + O2 + H2O | 14% activity compared to putrescine | Rhodococcus erythropolis NCIMB 11540 | 5-aminopentanal + NH3 + H2O2 | - |
? | |
1.4.3.10 | L-ornithine + O2 + H2O | 0.08% activity compared to putrescine | Rhodococcus erythropolis | ? | - |
? | |
1.4.3.10 | additional information | no activity with aromatic amines and amino alcohols | Rhodococcus erythropolis | ? | - |
? | |
1.4.3.10 | additional information | no activity with aromatic amines and amino alcohols | Rhodococcus erythropolis NCIMB 11540 | ? | - |
? | |
1.4.3.10 | putrescine + O2 + H2O | 100% activity | Rhodococcus erythropolis | 4-aminobutanal + NH3 + H2O2 | - |
? | |
1.4.3.10 | putrescine + O2 + H2O | 100% activity | Rhodococcus erythropolis NCIMB 11540 | 4-aminobutanal + NH3 + H2O2 | - |
? | |
1.4.3.10 | spermidine + O2 + H2O | 5.3% activity compared to putrescine | Rhodococcus erythropolis | 4-aminobutyraldehyde + 1,3-diaminopropane + H2O2 | - |
? | |
1.4.3.10 | spermidine + O2 + H2O | 5.3% activity compared to putrescine | Rhodococcus erythropolis NCIMB 11540 | 4-aminobutyraldehyde + 1,3-diaminopropane + H2O2 | - |
? | |
1.4.3.10 | spermine + O2 + H2O | 0.7% activity compared to putrescine | Rhodococcus erythropolis | N,N'-bis(3-aminopropyl)-4-aminobutanal + NH3 + H2O2 | - |
? | |
1.4.3.10 | spermine + O2 + H2O | 0.7% activity compared to putrescine | Rhodococcus erythropolis NCIMB 11540 | N,N'-bis(3-aminopropyl)-4-aminobutanal + NH3 + H2O2 | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.4.3.10 | homodimer | 2 * 54000, SDS-PAGE | Rhodococcus erythropolis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.4.3.10 | PuO | - |
Rhodococcus erythropolis |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.4.3.10 | 37 | 50 | at 50°C, half of the activity is lost after 2 h, while at 37°C, such a degree of inactivation is reached after 1 day | Rhodococcus erythropolis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.4.3.10 | 0.0198 | - |
L-ornithine | wild type enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 25°C | Rhodococcus erythropolis | |
1.4.3.10 | 0.18 | - |
spermine | wild type enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 25°C | Rhodococcus erythropolis | |
1.4.3.10 | 0.37 | - |
1,6-diaminohexane | wild type enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 25°C | Rhodococcus erythropolis | |
1.4.3.10 | 1.4 | - |
spermidine | wild type enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 25°C | Rhodococcus erythropolis | |
1.4.3.10 | 3.2 | - |
putrescine | mutant enzyme E324L, in 50 mM Tris-HCl buffer, pH 9.0, at 25°C | Rhodococcus erythropolis | |
1.4.3.10 | 3.9 | - |
cadaverine | wild type enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 25°C | Rhodococcus erythropolis | |
1.4.3.10 | 6.1 | - |
putrescine | mutant enzyme E324A, in 50 mM Tris-HCl buffer, pH 9.0, at 25°C | Rhodococcus erythropolis | |
1.4.3.10 | 7.5 | - |
2-hydroxyputrescine | wild type enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 25°C | Rhodococcus erythropolis | |
1.4.3.10 | 20.7 | - |
putrescine | wild type enzyme, in 50 mM Tris-HCl buffer, pH 9.0, at 25°C | Rhodococcus erythropolis | |
1.4.3.10 | 26.4 | - |
putrescine | wild type enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 25°C | Rhodococcus erythropolis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.4.3.10 | 8 | - |
around pH 8.0 | Rhodococcus erythropolis |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.4.3.10 | 6.4 | - |
below pH 6.4, no significant activity can be detected | Rhodococcus erythropolis |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.4.3.10 | FAD | contains non-covalently bound FAD | Rhodococcus erythropolis |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.4.3.10 | 0.0018 | - |
Aminoethanol | wild type enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 25°C | Rhodococcus erythropolis | |
1.4.3.10 | 0.064 | - |
1,3-diaminopropane | wild type enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 25°C | Rhodococcus erythropolis | |
1.4.3.10 | 0.12 | - |
n-butylamine | wild type enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 25°C | Rhodococcus erythropolis | |
1.4.3.10 | 0.66 | - |
ethylenediamine | wild type enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 25°C | Rhodococcus erythropolis | |
1.4.3.10 | 5.8 | - |
(2-aminoethyl)-trimethylammonium | wild type enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 25°C | Rhodococcus erythropolis |