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Literature summary extracted from

  • van Hellemond, E.W.; van Dijk, M.; Heuts, D.P.; Janssen, D.B.; Fraaije, M.W.
    Discovery and characterization of a putrescine oxidase from Rhodococcus erythropolis NCIMB 11540 (2008), Appl. Microbiol. Biotechnol., 78, 455-463.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.3.10 expressed in Escherichia coli Top10 cells Rhodococcus erythropolis

Protein Variants

EC Number Protein Variants Comment Organism
1.4.3.10 E324A the mutant enzyme shows activity with monoamines, which are not accepted by the wild type enzyme Rhodococcus erythropolis
1.4.3.10 E324L the mutant enzyme shows activity with monoamines, which are not accepted by the wild type enzyme Rhodococcus erythropolis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.3.10 (2-aminoethyl)-trimethylammonium competitive inhibitor Rhodococcus erythropolis
1.4.3.10 1,3-diaminopropane competitive inhibitor Rhodococcus erythropolis
1.4.3.10 Aminoethanol competitive inhibitor Rhodococcus erythropolis
1.4.3.10 ethylenediamine competitive inhibitor Rhodococcus erythropolis
1.4.3.10 additional information short diamines and monoamines strongly inhibit activity Rhodococcus erythropolis
1.4.3.10 n-butylamine competitive inhibitor Rhodococcus erythropolis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.10 0.0035
-
putrescine wild type enzyme, in 50 mM Tris-HCl buffer, pH 9.0, at 25°C Rhodococcus erythropolis
1.4.3.10 0.0082
-
putrescine wild type enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 25°C Rhodococcus erythropolis
1.4.3.10 0.018
-
cadaverine wild type enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 25°C Rhodococcus erythropolis
1.4.3.10 0.065
-
2-hydroxyputrescine wild type enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 25°C Rhodococcus erythropolis
1.4.3.10 0.1
-
spermidine wild type enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 25°C Rhodococcus erythropolis
1.4.3.10 0.17
-
1,6-diaminohexane wild type enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 25°C Rhodococcus erythropolis
1.4.3.10 0.33
-
L-ornithine wild type enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 25°C Rhodococcus erythropolis
1.4.3.10 0.47
-
spermine wild type enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 25°C Rhodococcus erythropolis
1.4.3.10 110
-
putrescine mutant enzyme E324A, in 50 mM Tris-HCl buffer, pH 9.0, at 25°C Rhodococcus erythropolis
1.4.3.10 110
-
putrescine mutant enzyme E324L, in 50 mM Tris-HCl buffer, pH 9.0, at 25°C Rhodococcus erythropolis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.3.10 54000
-
2 * 54000, SDS-PAGE Rhodococcus erythropolis
1.4.3.10 98750
-
theoretical molecular mass Rhodococcus erythropolis
1.4.3.10 108000
-
SDS-PAGE Rhodococcus erythropolis

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.10 Rhodococcus erythropolis B0F9F6 fragment
-
1.4.3.10 Rhodococcus erythropolis NCIMB 11540 B0F9F6 fragment
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.10 Q-Sepharose column chromatography Rhodococcus erythropolis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.10 1,6-diaminohexane + O2 + H2O 1.4% activity compared to putrescine Rhodococcus erythropolis 6-aminohexanal + NH3 + H2O2
-
?
1.4.3.10 2-hydroxyputrescine + O2 + H2O 28% activity compared to putrescine Rhodococcus erythropolis ?
-
?
1.4.3.10 4-amino-1-butanol + O2 + H2O 0.6% activity compared to putrescine Rhodococcus erythropolis 4-hydroxybutanal + NH3 + H2O2
-
?
1.4.3.10 cadaverine + O2 + H2O 14% activity compared to putrescine Rhodococcus erythropolis 5-aminopentanal + NH3 + H2O2
-
?
1.4.3.10 cadaverine + O2 + H2O 14% activity compared to putrescine Rhodococcus erythropolis NCIMB 11540 5-aminopentanal + NH3 + H2O2
-
?
1.4.3.10 L-ornithine + O2 + H2O 0.08% activity compared to putrescine Rhodococcus erythropolis ?
-
?
1.4.3.10 additional information no activity with aromatic amines and amino alcohols Rhodococcus erythropolis ?
-
?
1.4.3.10 additional information no activity with aromatic amines and amino alcohols Rhodococcus erythropolis NCIMB 11540 ?
-
?
1.4.3.10 putrescine + O2 + H2O 100% activity Rhodococcus erythropolis 4-aminobutanal + NH3 + H2O2
-
?
1.4.3.10 putrescine + O2 + H2O 100% activity Rhodococcus erythropolis NCIMB 11540 4-aminobutanal + NH3 + H2O2
-
?
1.4.3.10 spermidine + O2 + H2O 5.3% activity compared to putrescine Rhodococcus erythropolis 4-aminobutyraldehyde + 1,3-diaminopropane + H2O2
-
?
1.4.3.10 spermidine + O2 + H2O 5.3% activity compared to putrescine Rhodococcus erythropolis NCIMB 11540 4-aminobutyraldehyde + 1,3-diaminopropane + H2O2
-
?
1.4.3.10 spermine + O2 + H2O 0.7% activity compared to putrescine Rhodococcus erythropolis N,N'-bis(3-aminopropyl)-4-aminobutanal + NH3 + H2O2
-
?
1.4.3.10 spermine + O2 + H2O 0.7% activity compared to putrescine Rhodococcus erythropolis NCIMB 11540 N,N'-bis(3-aminopropyl)-4-aminobutanal + NH3 + H2O2
-
?

Subunits

EC Number Subunits Comment Organism
1.4.3.10 homodimer 2 * 54000, SDS-PAGE Rhodococcus erythropolis

Synonyms

EC Number Synonyms Comment Organism
1.4.3.10 PuO
-
Rhodococcus erythropolis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.3.10 37 50 at 50°C, half of the activity is lost after 2 h, while at 37°C, such a degree of inactivation is reached after 1 day Rhodococcus erythropolis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4.3.10 0.0198
-
L-ornithine wild type enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 25°C Rhodococcus erythropolis
1.4.3.10 0.18
-
spermine wild type enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 25°C Rhodococcus erythropolis
1.4.3.10 0.37
-
1,6-diaminohexane wild type enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 25°C Rhodococcus erythropolis
1.4.3.10 1.4
-
spermidine wild type enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 25°C Rhodococcus erythropolis
1.4.3.10 3.2
-
putrescine mutant enzyme E324L, in 50 mM Tris-HCl buffer, pH 9.0, at 25°C Rhodococcus erythropolis
1.4.3.10 3.9
-
cadaverine wild type enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 25°C Rhodococcus erythropolis
1.4.3.10 6.1
-
putrescine mutant enzyme E324A, in 50 mM Tris-HCl buffer, pH 9.0, at 25°C Rhodococcus erythropolis
1.4.3.10 7.5
-
2-hydroxyputrescine wild type enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 25°C Rhodococcus erythropolis
1.4.3.10 20.7
-
putrescine wild type enzyme, in 50 mM Tris-HCl buffer, pH 9.0, at 25°C Rhodococcus erythropolis
1.4.3.10 26.4
-
putrescine wild type enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 25°C Rhodococcus erythropolis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.3.10 8
-
around pH 8.0 Rhodococcus erythropolis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.4.3.10 6.4
-
below pH 6.4, no significant activity can be detected Rhodococcus erythropolis

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.3.10 FAD contains non-covalently bound FAD Rhodococcus erythropolis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.4.3.10 0.0018
-
Aminoethanol wild type enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 25°C Rhodococcus erythropolis
1.4.3.10 0.064
-
1,3-diaminopropane wild type enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 25°C Rhodococcus erythropolis
1.4.3.10 0.12
-
n-butylamine wild type enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 25°C Rhodococcus erythropolis
1.4.3.10 0.66
-
ethylenediamine wild type enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 25°C Rhodococcus erythropolis
1.4.3.10 5.8
-
(2-aminoethyl)-trimethylammonium wild type enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 25°C Rhodococcus erythropolis