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Literature summary extracted from

  • Kondo, T.; Morikawa, Y.; Hayashi, N.
    Purification and characterization of alcohol oxidase from Paecilomyces variotii isolated as a formaldehyde-resistant fungus (2008), Appl. Microbiol. Biotechnol., 77, 995-1002.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.3.13 1,10-phenanthroline 1 mM, 1.25fold activation Paecilomyces variotii
1.1.3.13 iodoacetamide 1 mM, 1.3fold activation Paecilomyces variotii

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.3.13 4-chloromercuribenzoate 0.1 mM, 31% inhibition Paecilomyces variotii
1.1.3.13 HgCl2 1 mM, 44% inhibition Paecilomyces variotii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.13 1.9
-
 methanol
-
 Paecilomyces variotii
1.1.3.13 3.8
-
 ethanol
-
 Paecilomyces variotii
1.1.3.13 4.9
-
 formaldehyde
-
 Paecilomyces variotii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.3.13 73000
-
 6 * 73000, SDS-PAGE Paecilomyces variotii
1.1.3.13 450000
-
 gel filtration Paecilomyces variotii

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.13 Paecilomyces variotii
-
 isolated as a formaldehyde-resistant fungus from wastewater containing formaldehyde
-
1.1.3.13 Paecilomyces variotii RI017
-
 isolated as a formaldehyde-resistant fungus from wastewater containing formaldehyde
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.13
-
 Paecilomyces variotii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.3.13 1.42
-
-
 Paecilomyces variotii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.13 ethanol + O2 106% of the activity with methanol Paecilomyces variotii acetaldehyde + H2O2
-
 ?
1.1.3.13 ethanol + O2 106% of the activity with methanol Paecilomyces variotii RI017 acetaldehyde + H2O2
-
 ?
1.1.3.13 formaldehyde + O2 28% of the activity with methanol Paecilomyces variotii formic acid + H2O2
-
 ?
1.1.3.13 formaldehyde + O2 28% of the activity with methanol Paecilomyces variotii RI017 formic acid + H2O2
-
 ?
1.1.3.13 methanol + O2
-
 Paecilomyces variotii formaldehyde + H2O2
-
 ?
1.1.3.13 methanol + O2
-
 Paecilomyces variotii RI017 formaldehyde + H2O2
-
 ?
1.1.3.13 n-propanol + O2 33% of the activity with methanol Paecilomyces variotii n-propanal + H2O2
-
 ?
1.1.3.13 n-propanol + O2 33% of the activity with methanol Paecilomyces variotii RI017 n-propanal + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.3.13 hexamer 6 * 73000, SDS-PAGE Paecilomyces variotii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.3.13 50
-
 30 min, enzyme retains full activity Paecilomyces variotii
1.1.3.13 60
-
 30 min, enzyme is almost completely inactivated Paecilomyces variotii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.13 6 10
-
 Paecilomyces variotii

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.3.13 5 10 stable Paecilomyces variotii

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.13 flavin noncovalently bound flavin different from FAD, FMN, and riboflavin Paecilomyces variotii