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Literature summary extracted from

  • Hilden, K.; Hakala, T.K.; Maijala, P.; Lundell, T.K.; Hatakka, A.
    Novel thermotolerant laccases produced by the white-rot fungus Physisporinus rivulosus (2007), Appl. Microbiol. Biotechnol., 77, 301-309.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.2 0.0028
-
 syringaldazine pH 3.0, 25°C, laccase Lac-4.8 Ceriporiopsis rivulosa
1.10.3.2 0.0107
-
 2,2'-azino-bis(3-ethylbenzathiazoline-6-sulfonate) pH 3.0, 25°C, laccase Lac-3.5 Ceriporiopsis rivulosa
1.10.3.2 0.017
-
 2,2'-azino-bis(3-ethylbenzathiazoline-6-sulfonate) pH 3.0, 25°C,laccase Lac-4.8 Ceriporiopsis rivulosa
1.10.3.2 0.027
-
 syringaldazine pH 3.0, 25°C, laccase Lac-3.5 Ceriporiopsis rivulosa
1.10.3.2 0.084
-
 2,6-dimethoxyphenol pH 3.0, 25°C, laccase Lac-4.8 Ceriporiopsis rivulosa
1.10.3.2 0.088
-
 2,6-dimethoxyphenol pH 3.0, 25°C, laccase Lac-3.5 Ceriporiopsis rivulosa
1.10.3.2 1.095
-
 guaiacol pH 3.0, 25°C, laccase Lac-3.5 Ceriporiopsis rivulosa
1.10.3.2 1.406
-
 guaiacol pH 3.0, 25°C, laccase Lac-4.8 Ceriporiopsis rivulosa

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.2 Ceriporiopsis rivulosa
-
-
-
1.10.3.2 Ceriporiopsis rivulosa T241i
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.2 two laccases with distinct pI values, Lac-3.5 and Lac-4.8, are purified from peptone-spruce sawdust-charcoal cultures Ceriporiopsis rivulosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.2 2,2'-azino-bis(3-ethylbenzathiazoline-6-sulfonate) + O2
-
 Ceriporiopsis rivulosa ?
-
 ?
1.10.3.2 2,2'-azino-bis(3-ethylbenzathiazoline-6-sulfonate) + O2
-
 Ceriporiopsis rivulosa T241i ?
-
 ?
1.10.3.2 2,6-dimethoxyphenol + O2
-
 Ceriporiopsis rivulosa ?
-
 ?
1.10.3.2 2,6-dimethoxyphenol + O2
-
 Ceriporiopsis rivulosa T241i ?
-
 ?
1.10.3.2 guaiacol + O2
-
 Ceriporiopsis rivulosa ?
-
 ?
1.10.3.2 guaiacol + O2
-
 Ceriporiopsis rivulosa T241i ?
-
 ?
1.10.3.2 syringaldazine + O2 i.e. 4-hydroxy-3,5-dimethoxybenzaldehyde azine Ceriporiopsis rivulosa ?
-
 ?
1.10.3.2 syringaldazine + O2 i.e. 4-hydroxy-3,5-dimethoxybenzaldehyde azine Ceriporiopsis rivulosa T241i ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.10.3.2 Lac-3.5
-
 Ceriporiopsis rivulosa
1.10.3.2 Lac-4.8
-
 Ceriporiopsis rivulosa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.10.3.2 50
-
 laccase Lac-4.8 shows thermal activation at 50°C and the enzyme activity after incubation is up to 120% of the original activity measured at 25°C Ceriporiopsis rivulosa
1.10.3.2 60
-
 Lac-3.5 and Lac-4.8 show thermal stability at up to 60°C Ceriporiopsis rivulosa
1.10.3.2 70
-
 half-life: 30 min, laccase Lac-4.8 Ceriporiopsis rivulosa
1.10.3.2 70
-
 half-life: 60 min, laccase Lac-3.5 Ceriporiopsis rivulosa
1.10.3.2 80
-
 20 min, 80% loss of activity Ceriporiopsis rivulosa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.10.3.2 71
-
 guaiacol pH 3.0, 25°C, laccase Lac-3.5 Ceriporiopsis rivulosa
1.10.3.2 82
-
 guaiacol pH 3.0, 25°C, laccase Lac-4.8 Ceriporiopsis rivulosa
1.10.3.2 124
-
 syringaldazine pH 3.0, 25°C, laccase Lac-4.8 Ceriporiopsis rivulosa
1.10.3.2 282
-
 syringaldazine pH 3.0, 25°C, laccase Lac-3.5 Ceriporiopsis rivulosa
1.10.3.2 286
-
 2,2'-azino-bis(3-ethylbenzathiazoline-6-sulfonate) pH 3.0, 25°C, laccase Lac-3.5 Ceriporiopsis rivulosa
1.10.3.2 347
-
 2,6-dimethoxyphenol pH 3.0, 25°C, laccase Lac-3.5 Ceriporiopsis rivulosa
1.10.3.2 409
-
 2,2'-azino-bis(3-ethylbenzathiazoline-6-sulfonate) pH 3.0, 25°C, laccase Lac-4.8 Ceriporiopsis rivulosa
1.10.3.2 481
-
 2,6-dimethoxyphenol pH 3.0, 25°C, laccase Lac-4.8 Ceriporiopsis rivulosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.2 3 3.5 laccase Lac-3.5 Ceriporiopsis rivulosa
1.10.3.2 3 3.5 laccase Lac-4.8 Ceriporiopsis rivulosa

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.10.3.2 Ceriporiopsis rivulosa isoelectric focusing, laccase Lac-3.5
-
 3.5
1.10.3.2 Ceriporiopsis rivulosa isoelectric focusing, laccase Lac-4.8
-
 4.8