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Literature summary extracted from

  • Orita, I.; Sakamoto, N.; Kato, N.; Yurimoto, H.; Sakai, Y.
    Bifunctional enzyme fusion of 3-hexulose-6-phosphate synthase and 6-phospho-3-hexuloisomerase (2007), Appl. Microbiol. Biotechnol., 76, 439-445.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.2.43 expressed in Escherichia coli Rosetta (DE3) cells, HPS only, or as fusion enzyme with 6-phospho-3-hexuloisomerase Mycobacterium gastri

Protein Variants

EC Number Protein Variants Comment Organism
5.3.1.27 additional information fusion gene construct of 3-hexulose-6-phosphate synthase and 6-phospho-3-hexuloisomerase. The gene product of 3-hexulose-6-phosphate synthase /6-phospho-3-hexuloisomerase exhibits both activities at room temperature and catalyzes the sequential reactions more efficiently than a simple mixture of the individual enzymes. The gene product of 6-phospho-3-hexuloisomerase /3-hexulose-6-phosphate synthase fails to display any enzyme activity. Escherichia coli strains harboring the 3-hexulose-6-phosphate synthase /6-phospho-3-hexuloisomerase gene consume formaldehyde more efficiently and exhibited better growth in a formaldehyde containing medium than the host strain Mycobacterium gastri

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.2.43 2.96
-
 formaldehyde purified recombinant enzyme, at 30°C Mycobacterium gastri

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.2.43 22000
-
 2 * 22000, SDS-PAGE Mycobacterium gastri
4.1.2.43 45000
-
 gel filtration Mycobacterium gastri
5.3.1.27 21000
-
 4 * 21000, SDS-PAGE, recombinant 6-phospho-3-hexuloisomerase Mycobacterium gastri
5.3.1.27 42000
-
 x * 42000, recombinant 3-hexulose-6-phosphate synthase /6-phospho-3-hexuloisomerasefusion enzyme Mycobacterium gastri
5.3.1.27 94000
-
 gel filtration, recombinant 6-phospho-3-hexuloisomerase Mycobacterium gastri

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.43 Mycobacterium gastri
-
-
-
4.1.2.43 Mycobacterium gastri MB19
-
-
-
5.3.1.27 Mycobacterium gastri Q9LBW5
-
-
5.3.1.27 Mycobacterium gastri MB19 Q9LBW5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.43 DEAE-Toyopearl column chromatography butyl-Toyopearl column chromatography Mycobacterium gastri

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.2.43 41.1
-
 cell free extract, recombinant HPS Mycobacterium gastri
4.1.2.43 70.9
-
 after 1.73fold purification, recombinant HPS Mycobacterium gastri
5.3.1.27 154
-
 3-hexulose-6-phosphate synthase/6-phospho-3-hexuloisomerasefusion enzyme, 30°C Mycobacterium gastri
5.3.1.27 563
-
 6-phospho-3-hexuloisomerase, 30°C Mycobacterium gastri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.43 D-ribulose 5-phosphate + formaldehyde
-
 Mycobacterium gastri D-arabino-hex-3-ulose 6-phosphate
-
 ?
4.1.2.43 D-ribulose 5-phosphate + formaldehyde
-
 Mycobacterium gastri MB19 D-arabino-hex-3-ulose 6-phosphate
-
 ?
5.3.1.27 D-arabino-3-hexulose 6-phosphate
-
 Mycobacterium gastri D-fructose 6-phosphate
-
 ?
5.3.1.27 D-arabino-3-hexulose 6-phosphate
-
 Mycobacterium gastri MB19 D-fructose 6-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.2.43 homodimer 2 * 22000, SDS-PAGE Mycobacterium gastri
5.3.1.27 ? x * 42000, recombinant 3-hexulose-6-phosphate synthase /6-phospho-3-hexuloisomerasefusion enzyme Mycobacterium gastri
5.3.1.27 tetramer 4 * 21000, SDS-PAGE, recombinant 6-phospho-3-hexuloisomerase Mycobacterium gastri

Synonyms

EC Number Synonyms Comment Organism
4.1.2.43 3-hexulose-6-phosphate synthase
-
 Mycobacterium gastri
4.1.2.43 HPS
-
 Mycobacterium gastri

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.2.43 40
-
 purified recombinant enzyme Mycobacterium gastri
5.3.1.27 30
-
 both recombinant 6-phospho-3-hexuloisomerase and 3-hexulose-6-phosphate synthase /6-phospho-3-hexuloisomerase fusion enzyme Mycobacterium gastri