Any feedback?
Literature summary extracted from
- Escherichia coli hydrogenase 3 is a reversible enzyme possessing hydrogen uptake and synthesis activities (2007), Appl. Microbiol. Biotechnol., 76, 1035-1042.
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 1.12.99.6 | additional information | the uptake activity in mutant HD705/pBS(Kan), which is defective in hydrogenase 3, is 4.4fold lower than that in wild-type enzyme, the hydrogen uptake activity in the hydrogenase 1 and 2 double mutant (hyaB hybC) is reduced 2.7fold by addition of the hycE mutation (hyaB hybC hycE) | Escherichia coli |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.12.99.6 | CN- | contains two CN- molecules at the active site | Escherichia coli |  |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.12.99.6 | 65000 | - |
hydrogenase 3, SDS-PAGE | Escherichia coli |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.12.99.6 | Escherichia coli | - |
strains BW25113 and MC4100 | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.12.99.6 | 0.008 | - |
hydrogen uptake specific activity of the hydrogenase 3 lacking mutant enzyme HD705/pBS(Kan), using methyl viologen as a substrate | Escherichia coli |
| 1.12.99.6 | 0.035 | - |
hydrogen uptake specific activity of the wild type enzyme, using methyl viologen as a substrate | Escherichia coli |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.12.99.6 | H2 + acceptor | - |
Escherichia coli | acceptor-H2 | - |
? | |
| 1.12.99.6 | H2 + oxidized methyl viologen | - |
Escherichia coli | H+ + reduced methyl viologen | - |
r | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.12.99.6 | HycE | hydrogenase 3 | Escherichia coli |
| 1.12.99.6 | hydrogen uptake hydrogenase | - |
Escherichia coli |
| 1.12.99.6 | hydrogenase 1 | hydrogen uptake activity only | Escherichia coli |
| 1.12.99.6 | hydrogenase 2 | hydrogen uptake activity only | Escherichia coli |
| 1.12.99.6 | hydrogenase 3 | hydrogen uptake and synthesis activity | Escherichia coli |
| 1.12.99.6 | [NiFe]-hydrogenase | - |
Escherichia coli |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
