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Literature summary extracted from
- Identification of promoters of two dehydrogenase genes in Ketogulonicigenium vulgare DSM 4025 and their strength comparison in K. vulgare and Escherichia coli (2007), Appl. Microbiol. Biotechnol., 75, 1127-1132.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 1.1.99.12 | food industry | L-sorbose can be catalyzed into L-ascorbic acid by Ketogulonicigenium vulgare, vitamin C industry | Escherichia coli |
| 1.1.99.12 | food industry | L-sorbose can be catalyzed into L-ascorbic acid by Ketogulonicigenium vulgare, vitamin C industry | Ketogulonicigenium vulgare |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.1.99.12 | constructs with and without sequences of promoters, identification of the transcription initiation site | Escherichia coli |
| 1.1.99.12 | expression in Escherichia coli, constructs with and without sequences of promoters, identification of the transcription initiation site | Ketogulonicigenium vulgare |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.99.12 | Escherichia coli | - |
- |
- |
| 1.1.99.12 | Ketogulonicigenium vulgare | - |
DSM 4025 | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.12 | 10.3 | - |
promoters has strong activity | Escherichia coli |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.99.12 | L-sorbose + 2,6-dichlorophenolindophenol | 2,6-dichlorophenol-indophenol decolouration assay | Escherichia coli | L-sorbosone + reduced 2,6-dichlorophenolindophenol | - |
? |  |
| 1.1.99.12 | L-sorbose + 2,6-dichlorophenolindophenol | 2,6-dichlorophenol-indophenol decolouration assay | Ketogulonicigenium vulgare | L-sorbosone + reduced 2,6-dichlorophenolindophenol | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.1.99.12 | sorbose dehydrogenase | SDH | Escherichia coli |
| 1.1.99.12 | sorbose dehydrogenase | SDH | Ketogulonicigenium vulgare |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.99.12 | pyrroloquinoline quinone | - |
Escherichia coli | |
| 1.1.99.12 | pyrroloquinoline quinone | - |
Ketogulonicigenium vulgare | |
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Release 2023.1 (January 2023)
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