EC Number | Cloned (Comment) | Organism |
---|---|---|
1.3.1.12 | expressed in Escherichia coli | Zymomonas mobilis |
4.2.1.51 | expressed in Escherichia coli, PB12 strain, pCR-BluntII-TOPO, additional strains and plasmids listed | Zymomonas mobilis |
5.4.99.5 | expressed in Escherichia coli strain FA114 | Escherichia coli |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.3.1.12 | L-Tyr | L-Tyr inhibits TyrAp activity by 90% at concentrations higher than 0.5 mM | Zymomonas mobilis | |
1.3.1.79 | additional information | TyrC is not sensitive to L-Tyr inhibition | Zymomonas mobilis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.3.1.12 | Zymomonas mobilis | - |
- |
- |
1.3.1.79 | Zymomonas mobilis | - |
strain ATCC 31821 | - |
4.2.1.51 | Zymomonas mobilis | Q5NLV8 | encoded by pheA (ZMO1678) gene; ATCC 31821 wild-type strain | - |
4.2.1.51 | Zymomonas mobilis ATCC 31821 | Q5NLV8 | encoded by pheA (ZMO1678) gene; ATCC 31821 wild-type strain | - |
5.4.99.5 | Escherichia coli | - |
- |
- |
5.4.99.5 | Escherichia coli JM101 | - |
- |
- |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.3.1.79 | additional information | - |
TyrC has a specific activity of 1.54 IU/mg protein, while in the presence of 0.6 mM L-Tyr the specific activity was 1.34 IU/mg protein | Zymomonas mobilis |
4.2.1.51 | additional information | - |
comparison of prephenate dehydratase isolated from Zymomonas mobilis to those of Escherichia coli with regard to the capacity to produce L-tyrosine in Escherichia coli strains modified to increase the carbon flow to chorismate, kinetic and stoichiometric parameters determined in shake flask experiments, parameters determined from data generated in bioreactor experiments, possibility to employ feedback inhibition-insensitive enzymes for strain development as part of a metabolic engineering strategy for L-tyrosine production | Zymomonas mobilis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.1.12 | prephenate + NAD+ | - |
Zymomonas mobilis | 4-hydroxyphenylpyruvate + NADH + CO2 | - |
? | |
1.3.1.79 | L-arogenate + NAD(P)+ | - |
Zymomonas mobilis | L-tyrosine + NAD(P)H + CO2 | - |
? | |
1.3.1.79 | prephenate + NAD(P)+ | - |
Zymomonas mobilis | p-hydroxyphenylpyruvate + NAD(P)H + CO2 | - |
? | |
5.4.99.5 | Chorismate | - |
Escherichia coli | Prephenate | - |
? | |
5.4.99.5 | Chorismate | - |
Escherichia coli JM101 | Prephenate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.3.1.12 | chorismate mutase-prephenate dehydratase | - |
Zymomonas mobilis |
1.3.1.12 | CM-TyrAp | - |
Zymomonas mobilis |
1.3.1.79 | cyclohexadienyl dehydrogenase | - |
Zymomonas mobilis |
1.3.1.79 | TyrAc | - |
Zymomonas mobilis |
1.3.1.79 | TyrC | - |
Zymomonas mobilis |
5.4.99.5 | chorismate mutase-prephenate dehydrogenase | bifunctional enzyme | Escherichia coli |
5.4.99.5 | CM-prephenate dehydratase | bifunctional enzyme | Escherichia coli |
5.4.99.5 | CM-TyrAp | bifunctional enzyme | Escherichia coli |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.3.1.12 | NAD+ | - |
Zymomonas mobilis | |
1.3.1.79 | NAD(P)+ | - |
Zymomonas mobilis |