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Literature summary extracted from

  • Tsukihara, T.; Honda, Y.; Sakai, R.; Watanabe, T.; Watanabe, T.
    Mechanism for oxidation of high-molecular-weight substrates by a fungal versatile peroxidase, MnP2 (2008), Appl. Environ. Microbiol., 74, 2873-2881.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
1.11.1.16 E249D no catalytic activity Pleurotus ostreatus
1.11.1.16 E249Q no catalytic activity Pleurotus ostreatus
1.11.1.16 Q266F kinetic properties for H2O2 almost identical to those of wild-type, less than half the RNase A-oxidizing activity of wild-type Pleurotus ostreatus
1.11.1.16 R263D no catalytic activity Pleurotus ostreatus
1.11.1.16 R263N kinetic properties for H2O2 almost identical to those of wild-type, additional N-glycosylation Pleurotus ostreatus
1.11.1.16 V166/168L kinetic properties for H2O2 almost identical to those of wild-type Pleurotus ostreatus
1.11.1.16 W170A kinetic properties for H2O2 almost identical to those of wild-type, no oxidation of veratryl alcohol, decrease in oxodation of RNase A Pleurotus ostreatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.16 0.007
-
 H2O2 mutant V166/168L Pleurotus ostreatus
1.11.1.16 0.0093
-
 H2O2 mutant Q266F Pleurotus ostreatus
1.11.1.16 0.0098
-
 H2O2 mutant R263N Pleurotus ostreatus
1.11.1.16 0.0099
-
 H2O2 mutant W170A Pleurotus ostreatus
1.11.1.16 0.0103
-
 H2O2 wild-type Pleurotus ostreatus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.11.1.16 Manganese Km value 0.022 for wild-type, 0.021 for mutant W170A, 0.022 for mutant R263N, 0.026 for mutant Q266F, 0.027 for mutant V166/168L Pleurotus ostreatus

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.16 Pleurotus ostreatus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.11.1.16 glycoprotein
-
 Pleurotus ostreatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.16 Poly R-478 + H2O2 decolorization of the dye Pleurotus ostreatus ?
-
 ?
1.11.1.16 RNase A + H2O2
-
 Pleurotus ostreatus ?
-
 ?
1.11.1.16 veratryl alcohol + H2O2 + H+
-
 Pleurotus ostreatus veratraldehyde + H2O
-
 ?