EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.14.20.1 | C281F | substrate ampicillin, 191%, penicillin G, 264%, phenethicillin, 300%, and carbenicillin, 518% of wild-type activity | Streptomyces clavuligerus |
1.14.20.1 | C281Y | substrate ampicillin, 166%, penicillin G, 251%, phenethicillin, 280%, and carbenicillin, 572% of wild-type activity | Streptomyces clavuligerus |
1.14.20.1 | C281Y/I305M | substrate ampicillin, 491%, penicillin G, 495%, phenethicillin, 1109%, and carbenicillin, 1347% of wild-type activity | Streptomyces clavuligerus |
1.14.20.1 | C281Y/N304A | substrate ampicillin, 294%, penicillin G, 383%, phenethicillin, 714%, and carbenicillin, 911% of wild-type activity | Streptomyces clavuligerus |
1.14.20.1 | C281Y/N304K | substrate ampicillin, 376%, penicillin G, 428%, phenethicillin, 948%, and carbenicillin, 1180% of wild-type activity | Streptomyces clavuligerus |
1.14.20.1 | C281Y/N304L | substrate ampicillin, 330%, penicillin G, 211%, phenethicillin, 471%, and carbenicillin, 1001% of wild-type activity | Streptomyces clavuligerus |
1.14.20.1 | C281Y/N304M | substrate ampicillin, 225%, penicillin G, 277%, phenethicillin, 428%, and carbenicillin, 485% of wild-type activity | Streptomyces clavuligerus |
1.14.20.1 | C281Y/N304R | substrate ampicillin, 430%, penicillin G, 441%, phenethicillin, 1041%, and carbenicillin, 1309% of wild-type activity | Streptomyces clavuligerus |
1.14.20.1 | C281Y/R306L | substrate ampicillin, 394%, penicillin G, 191%, phenethicillin, 555%, and carbenicillin, 1010% of wild-type activity | Streptomyces clavuligerus |
1.14.20.1 | C281Y/R307L | substrate ampicillin, 274%, penicillin G, 334%, phenethicillin, 520%, and carbenicillin, 786% of wild-type activity | Streptomyces clavuligerus |
1.14.20.1 | I305M | substrate ampicillin, 313%, penicillin G, 234%, phenethicillin, 318%, and carbenicillin, 450% of wild-type activity | Streptomyces clavuligerus |
1.14.20.1 | N304A | substrate ampicillin, 192%, penicillin G, 163%, phenethicillin, 163%, and carbenicillin, 242% of wild-type activity | Streptomyces clavuligerus |
1.14.20.1 | N304A/R306L | substrate ampicillin, 214%, penicillin G, 114%, phenethicillin, 319%, and carbenicillin, 393% of wild-type activity | Streptomyces clavuligerus |
1.14.20.1 | N304K | substrate ampicillin, 270%, penicillin G, 237%, phenethicillin, 211%, an carbenicillin, 404% of wild-type activity | Streptomyces clavuligerus |
1.14.20.1 | N304K/R306L | substrate ampicillin, 245%, penicillin G, 80%, phenethicillin, 380%, and carbenicillin, 482% of wild-type activity | Streptomyces clavuligerus |
1.14.20.1 | N304L | substrate ampicillin, 163%, penicillin G, 129%, phenethicillin, 173%, and carbenicillin, 235% of wild-type activity | Streptomyces clavuligerus |
1.14.20.1 | N304L/R306L | substrate ampicillin, 145%, penicillin G, 40%, phenethicillin, 185%, and carbenicillin, 218% of wild-type activity | Streptomyces clavuligerus |
1.14.20.1 | N304M | substrate ampicillin, 120%, penicillin G,92%, phenethicillin, 127%, and carbenicillin, 161% of wild-type activity | Streptomyces clavuligerus |
1.14.20.1 | N304M/R306L | substrate ampicillin, 194%, penicillin G, 72%, phenethicillin, 245%, and carbenicillin, 232% of wild-type activity | Streptomyces clavuligerus |
1.14.20.1 | N304R | substrate ampicillin, 346%, penicillin G, 263%, phenethicillin, 360%, and carbenicillin, 398% of wild-type activity | Streptomyces clavuligerus |
1.14.20.1 | N304R/R306L | substrate ampicillin, 256%, penicillin G, 92%, phenethicillin, 344%, and carbenicillin, 514% of wild-type activity | Streptomyces clavuligerus |
1.14.20.1 | R306L | substrate ampicillin, 186%, penicillin G, 105%, phenethicillin, 173%, and carbenicillin, 283% of wild-type activity | Streptomyces clavuligerus |
1.14.20.1 | R307L | substrate ampicillin, 133%, penicillin G, 124%, phenethicillin, 122%, and carbenicillin, 108% of wild-type activity | Streptomyces clavuligerus |
1.14.20.1 | V275I | substrate ampicillin, 129%, penicillin G, 157%, phenethicillin, 150%, and carbenicillin, 227% of wild-type activity | Streptomyces clavuligerus |
1.14.20.1 | V275I/I305M | substrate ampicillin, 406%, penicillin G, 420%, phenethicillin, 685%, and carbenicillin, 1057% of wild-type activity | Streptomyces clavuligerus |
1.14.20.1 | V275I/N304A | substrate ampicillin, 265%, penicillin G, 233%, phenethicillin, 330%, and carbenicillin,721% of wild-type activity | Streptomyces clavuligerus |
1.14.20.1 | V275I/N304K | substrate ampicillin, 334%, penicillin G, 294%, phenethicillin, 635%, and carbenicillin, 867% of wild-type activity | Streptomyces clavuligerus |
1.14.20.1 | V275I/N304L | substrate ampicillin, 225%, penicillin G, 141%, phenethicillin, 245%, and carbenicillin, 524% of wild-type activity | Streptomyces clavuligerus |
1.14.20.1 | V275I/N304M | substrate ampicillin, 160%, penicillin G, 174%, phenethicillin, 261%, and carbenicillin, 230% of wild-type activity | Streptomyces clavuligerus |
1.14.20.1 | V275I/N304R | substrate ampicillin, 360%, penicillin G, 381%, phenethicillin, 698%, and carbenicillin,814% of wild-type activity | Streptomyces clavuligerus |
1.14.20.1 | V275I/R306L | substrate ampicillin, 343%, penicillin G, 168%, phenethicillin, 379%, and carbenicillin, 665% of wild-type activity | Streptomyces clavuligerus |
1.14.20.1 | V275I/R307L | substrate ampicillin, 172%, penicillin G, 169%, phenethicillin, 238%, and carbenicillin, 333% of wild-type activity | Streptomyces clavuligerus |
1.14.20.1 | V275L | substrate ampicillin, 85%, penicillin G, 130%, phenethicillin, 99%, and carbenicillin, 113% of wild-type activity | Streptomyces clavuligerus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.20.1 | 0.05 | - |
ampicillin | mutant C281Y7N304R, pH 7.4, 30°C | Streptomyces clavuligerus | |
1.14.20.1 | 0.11 | - |
ampicillin | mutant V275I/N304R, pH 7.4, 30°C | Streptomyces clavuligerus | |
1.14.20.1 | 0.12 | - |
ampicillin | mutant N304K/R306L, pH 7.4, 30°C | Streptomyces clavuligerus | |
1.14.20.1 | 0.13 | - |
ampicillin | mutant C281Y/N304K, pH 7.4, 30°C | Streptomyces clavuligerus | |
1.14.20.1 | 0.15 | - |
ampicillin | mutant C281Y/R306L, pH 7.4, 30°C | Streptomyces clavuligerus | |
1.14.20.1 | 0.19 | - |
ampicillin | mutant N304R/R306L, pH 7.4, 30°C | Streptomyces clavuligerus | |
1.14.20.1 | 0.2 | - |
ampicillin | mutant N304A/R306L, pH 7.4, 30°C | Streptomyces clavuligerus | |
1.14.20.1 | 0.21 | - |
ampicillin | mutant C281Y/I305M, pH 7.4, 30°C | Streptomyces clavuligerus | |
1.14.20.1 | 0.26 | - |
ampicillin | mutant N304R, pH 7.4, 30°C | Streptomyces clavuligerus | |
1.14.20.1 | 0.34 | - |
ampicillin | mutant C281Y/N304L, pH 7.4, 30°C | Streptomyces clavuligerus | |
1.14.20.1 | 0.36 | - |
ampicillin | mutant N304M/R306L, pH 7.4, 30°C | Streptomyces clavuligerus | |
1.14.20.1 | 0.38 | - |
ampicillin | mutant C281Y/N304A, pH 7.4, 30°C | Streptomyces clavuligerus | |
1.14.20.1 | 0.38 | - |
ampicillin | mutant V275I7N304K, pH 7.4, 30°C | Streptomyces clavuligerus | |
1.14.20.1 | 0.4 | - |
ampicillin | mutant N304L/R306L, pH 7.4, 30°C | Streptomyces clavuligerus | |
1.14.20.1 | 0.51 | - |
ampicillin | mutant V275I/R306L, pH 7.4, 30°C | Streptomyces clavuligerus | |
1.14.20.1 | 0.54 | - |
ampicillin | mutant N304K, pH 7.4, 30°C | Streptomyces clavuligerus | |
1.14.20.1 | 0.68 | - |
ampicillin | mutant V275I/N304A, pH 7.4, 30°C | Streptomyces clavuligerus | |
1.14.20.1 | 0.7 | - |
ampicillin | mutant V275I/I305M, pH 7.4, 30°C | Streptomyces clavuligerus | |
1.14.20.1 | 0.8 | - |
ampicillin | mutant C281Y/N304M, pH 7.4, 30°C | Streptomyces clavuligerus | |
1.14.20.1 | 1 | - |
ampicillin | mutant I305M, pH 7.4, 30°C | Streptomyces clavuligerus | |
1.14.20.1 | 1.01 | - |
ampicillin | mutant R306L, pH 7.4, 30°C | Streptomyces clavuligerus | |
1.14.20.1 | 1.02 | - |
ampicillin | mutant C281Y, pH 7.4, 30°C | Streptomyces clavuligerus | |
1.14.20.1 | 1.04 | - |
ampicillin | mutant V275I/N304L, pH 7.4, 30°C | Streptomyces clavuligerus | |
1.14.20.1 | 1.09 | - |
ampicillin | mutant N304A, pH 7.4, 30°C | Streptomyces clavuligerus | |
1.14.20.1 | 1.2 | - |
ampicillin | mutant C281Y/R307L, pH 7.4, 30°C | Streptomyces clavuligerus | |
1.14.20.1 | 1.35 | - |
ampicillin | mutant C281F, pH 7.4, 30°C | Streptomyces clavuligerus | |
1.14.20.1 | 1.65 | - |
ampicillin | mutant N304L, pH 7.4, 30°C | Streptomyces clavuligerus | |
1.14.20.1 | 3.41 | - |
ampicillin | mutant V275I, pH 7.4, 30°C | Streptomyces clavuligerus | |
1.14.20.1 | 3.6 | - |
ampicillin | mutant V275I/N304M, pH 7.4, 30°C | Streptomyces clavuligerus | |
1.14.20.1 | 4.26 | - |
ampicillin | mutant V275L, pH 7.4, 30°C | Streptomyces clavuligerus | |
1.14.20.1 | 4.27 | - |
ampicillin | mutant V275I/R307L, pH 7.4, 30°C | Streptomyces clavuligerus | |
1.14.20.1 | 5.12 | - |
ampicillin | mutant N304M, pH 7.4, 30°C | Streptomyces clavuligerus | |
1.14.20.1 | 5.96 | - |
ampicillin | mutant R307L, pH 7.4, 30°C | Streptomyces clavuligerus | |
1.14.20.1 | 6.94 | - |
ampicillin | wild type, pH 7.4, 30°C | Streptomyces clavuligerus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.20.1 | Streptomyces clavuligerus | P18548 | - |
- |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.14.20.1 | 0.76 | - |
substrate carbenicillin, wild-type, pH 7.4, 30°C | Streptomyces clavuligerus |
1.14.20.1 | 3.57 | - |
substrate phenethicillin, wild-type, pH 7.4, 30°C | Streptomyces clavuligerus |
1.14.20.1 | 5.67 | - |
substrate ampicillin, wild-type, pH 7.4, 30°C | Streptomyces clavuligerus |
1.14.20.1 | 6.72 | - |
substrate penicillin G, wild-type, pH 7.4, 30°C | Streptomyces clavuligerus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.20.1 | ampicillin + 2-oxoglutarate + O2 | - |
Streptomyces clavuligerus | ? + succinate + CO2 | - |
? | |
1.14.20.1 | carbenicillin + 2-oxoglutarate + O2 | - |
Streptomyces clavuligerus | ? + succinate + CO2 | - |
? | |
1.14.20.1 | penicillin G + 2-oxoglutarate + O2 | - |
Streptomyces clavuligerus | ? + succinate + CO2 | - |
? | |
1.14.20.1 | phenethicillin + 2-oxoglutarate + O2 | - |
Streptomyces clavuligerus | ? + succinate + CO2 | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.20.1 | 0.026 | - |
ampicillin | mutant N304M/R306L, pH 7.4, 30°C | Streptomyces clavuligerus | |
1.14.20.1 | 0.029 | - |
ampicillin | mutant N304L/R306L, pH 7.4, 30°C | Streptomyces clavuligerus | |
1.14.20.1 | 0.03 | - |
ampicillin | mutant N304R/R306L, pH 7.4, 30°C | Streptomyces clavuligerus | |
1.14.20.1 | 0.032 | - |
ampicillin | mutant V275L, pH 7.4, 30°C | Streptomyces clavuligerus | |
1.14.20.1 | 0.033 | - |
ampicillin | mutant N304K/R306L, pH 7.4, 30°C | Streptomyces clavuligerus | |
1.14.20.1 | 0.034 | - |
ampicillin | mutant N304A/R306L, pH 7.4, 30°C | Streptomyces clavuligerus | |
1.14.20.1 | 0.037 | - |
ampicillin | mutant C281Y/R306L, pH 7.4, 30°C | Streptomyces clavuligerus | |
1.14.20.1 | 0.037 | - |
ampicillin | mutant V275I/R306L, pH 7.4, 30°C | Streptomyces clavuligerus | |
1.14.20.1 | 0.038 | - |
ampicillin | mutant N304K, pH 7.4, 30°C | Streptomyces clavuligerus | |
1.14.20.1 | 0.039 | - |
ampicillin | mutant V275I/N304R, pH 7.4, 30°C | Streptomyces clavuligerus | |
1.14.20.1 | 0.04 | - |
ampicillin | mutant N304A, pH 7.4, 30°C | Streptomyces clavuligerus | |
1.14.20.1 | 0.04 | - |
ampicillin | mutant V275I7N304K, pH 7.4, 30°C | Streptomyces clavuligerus | |
1.14.20.1 | 0.041 | - |
ampicillin | mutant N304L, pH 7.4, 30°C | Streptomyces clavuligerus | |
1.14.20.1 | 0.042 | - |
ampicillin | mutant C281F, pH 7.4, 30°C | Streptomyces clavuligerus | |
1.14.20.1 | 0.042 | - |
ampicillin | mutant V275I/N304A, pH 7.4, 30°C | Streptomyces clavuligerus | |
1.14.20.1 | 0.043 | - |
ampicillin | mutant C281Y/N304L, pH 7.4, 30°C | Streptomyces clavuligerus | |
1.14.20.1 | 0.043 | - |
ampicillin | mutant C281Y7N304R, pH 7.4, 30°C | Streptomyces clavuligerus | |
1.14.20.1 | 0.043 | - |
ampicillin | mutant V275I/N304L, pH 7.4, 30°C | Streptomyces clavuligerus | |
1.14.20.1 | 0.044 | - |
ampicillin | mutant C281Y, pH 7.4, 30°C | Streptomyces clavuligerus | |
1.14.20.1 | 0.044 | - |
ampicillin | mutant V275I, pH 7.4, 30°C | Streptomyces clavuligerus | |
1.14.20.1 | 0.046 | - |
ampicillin | mutant C281Y/N304K, pH 7.4, 30°C | Streptomyces clavuligerus | |
1.14.20.1 | 0.046 | - |
ampicillin | mutant C281Y/R307L, pH 7.4, 30°C | Streptomyces clavuligerus | |
1.14.20.1 | 0.047 | - |
ampicillin | mutant C281Y/N304M, pH 7.4, 30°C | Streptomyces clavuligerus | |
1.14.20.1 | 0.048 | - |
ampicillin | mutant V275I/N304M, pH 7.4, 30°C | Streptomyces clavuligerus | |
1.14.20.1 | 0.049 | - |
ampicillin | mutant V275I/R307L, pH 7.4, 30°C | Streptomyces clavuligerus | |
1.14.20.1 | 0.05 | - |
ampicillin | mutant R306L, pH 7.4, 30°C | Streptomyces clavuligerus | |
1.14.20.1 | 0.05 | - |
ampicillin | mutant R307L, pH 7.4, 30°C | Streptomyces clavuligerus | |
1.14.20.1 | 0.052 | - |
ampicillin | mutant C281Y/N304A, pH 7.4, 30°C | Streptomyces clavuligerus | |
1.14.20.1 | 0.052 | - |
ampicillin | mutant N304R, pH 7.4, 30°C | Streptomyces clavuligerus | |
1.14.20.1 | 0.054 | - |
ampicillin | mutant N304M, pH 7.4, 30°C | Streptomyces clavuligerus | |
1.14.20.1 | 0.059 | - |
ampicillin | wild type, pH 7.4, 30°C | Streptomyces clavuligerus | |
1.14.20.1 | 0.104 | - |
ampicillin | mutant I305M, pH 7.4, 30°C | Streptomyces clavuligerus | |
1.14.20.1 | 0.105 | - |
ampicillin | mutant V275I/I305M, pH 7.4, 30°C | Streptomyces clavuligerus | |
1.14.20.1 | 0.122 | - |
ampicillin | mutant C281Y/I305M, pH 7.4, 30°C | Streptomyces clavuligerus |