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Literature summary extracted from

  • Jouanneau, Y.; Micoud, J.; Meyer, C.
    Purification and characterization of a three-component salicylate 1-hydroxylase from Sphingomonas sp. strain CHY-1 (2007), Appl. Environ. Microbiol., 73, 7515-7521.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.13.1 1-hydroxy-2-naphthoate strong competitive inhibition of salicylate hydroxylation Sphingomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.1 0.0011
-
 salicylate 30°C, pH 7.0 Sphingomonas sp.
1.14.13.1 0.028
-
 anthranilate 30°C, pH 7.0 Sphingomonas sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.13.1 Iron three-component Fe-S protein complex: the oxygenase component, designated PhnII, exhibits an alpha3beta3 heterohexameric structure and contains one Rieske-type [2Fe-2S] cluster and one mononuclear iron per alpha subunit Sphingomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.1 Sphingomonas sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.1 oxygenase component PhnII Sphingomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.1 1-hydroxy-2-naphthoate + NADH + 2 H+ + O2 137% of the activity with salicylate Sphingomonas sp. ?
-
 ?
1.14.13.1 2,4-dihydroxybenzoate + NADH + 2 H+ + O2 91% of the activity with salicylate Sphingomonas sp. 1,2,4-trihydroxybenzene + NAD+ + H2O + CO2
-
 ?
1.14.13.1 2,6-dihydroxybenzoate + NADH + 2 H+ + O2 70% of the activity with salicylate Sphingomonas sp. 1,2,3-trihydroxybenzene + NAD+ + H2O + CO2
-
 ?
1.14.13.1 2-hydroxy-1-naphthoate + NADH + 2 H+ + O2 160% of the activity with salicylate Sphingomonas sp. ?
-
 ?
1.14.13.1 3-methylsalicylate + NADH + 2 H+ + O2 76% of the activity with salicylate Sphingomonas sp. 3-methylcatechol + NAD+ + H2O + CO2
-
 ?
1.14.13.1 4-methylsalicylate + NADH + 2 H+ + O2 108% of the activity with salicylate Sphingomonas sp. 4-methylcatechol + NAD+ + H2O + CO2
-
 ?
1.14.13.1 5-chlorosalicylate + NADH + 2 H+ + O2 46% of the activity with salicylate Sphingomonas sp. 4-chlorocatechol + NAD+ + H2O + CO2
-
 ?
1.14.13.1 5-methylsalicylate + NADH + 2 H+ + O2 68.5% of the activity with salicylate Sphingomonas sp. 4-methylcatechol + NAD+ + H2O + CO2
-
 ?
1.14.13.1 5-nitrosalicylate + NADH + 2 H+ + O2 32% of the activity with salicylate Sphingomonas sp. 4-nitrocatechol + NAD+ + H2O + CO2
-
 ?
1.14.13.1 anthranilate + NADH + 2 H+ + O2 67% of the activity with salicylate Sphingomonas sp. 2-aminophenol + NAD+ + H2O + CO2
-
 ?
1.14.13.1 gentisate + NADH + 2 H+ + O2 99% of the activity with salicylate Sphingomonas sp. ?
-
 ?
1.14.13.1 additional information 1-hydroxy-2-naphthoate, which is an intermediate in phenanthrene degradation, is not hydroxylated by PhnII, but it induces a high rate of uncoupled oxidation of NADH Sphingomonas sp. ?
-
 ?
1.14.13.1 salicylate + NADH + 2 H+ + O2
-
 Sphingomonas sp. catechol + NAD+ + H2O + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.13.1 More three-component Fe-S protein complex: the oxygenase component, designated PhnII, exhibits an alpha3beta3 heterohexameric structure and contains one Rieske-type [2Fe-2S] cluster and one mononuclear iron per alpha subunit. In the presence of purified reductase (PhnA4) and ferredoxin (PhnA3) components, PhnII catalyzes the hydroxylation of salicylate to catechol Sphingomonas sp.

Synonyms

EC Number Synonyms Comment Organism
1.14.13.1 salicylate 1-hydroxylase
-
 Sphingomonas sp.

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.14.13.1 0.00068
-
 1-hydroxy-2-naphthoate 30°C, pH 7.0 Sphingomonas sp.