Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Yasuhira, K.; Tanaka, Y.; Shibata, H.; Kawashima, Y.; Ohara, A.; Kato, D.; Takeo, M.; Negoro, S.
    6-Aminohexanoate oligomer hydrolases from the alkalophilic bacteria Agromyces sp. strain KY5R and Kocuria sp. strain KY2 (2007), Appl. Environ. Microbiol., 73, 7099-7102.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.46
-
 Arthrobacter sp.
3.5.1.46
-
 Agromyces sp.
3.5.1.46
-
 Kocuria sp.
3.5.1.117 expression in Escherichia coli Agromyces sp.
3.5.1.117 expression in Escherichia coli Kocuria sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.46 additional information
-
 additional information Km for 6-aminohexanoate cyclic-oligomer: 0.44 mg/ml Kocuria sp.
3.5.1.46 additional information
-
 additional information Km for 6-aminohexanoate cyclic-oligomer: 0.49 mg/ml Agromyces sp.
3.5.1.46 additional information
-
 additional information Km for 6-aminohexanoate cyclic-oligomer: 3.7 mg/ml Arthrobacter sp.
3.5.1.117 additional information
-
 6-aminohexanoate cyclic oligomer Km-value for 6-aminohexanoate cyclic oligomer: 0.44 mg/ml, pH 7.0, 30°C Kocuria sp.
3.5.1.117 additional information
-
 6-aminohexanoate cyclic oligomer Km-value for 6-aminohexanoate cyclic oligomer: 0.49 mg/ml, pH 7.0, 30°C Agromyces sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.117 9000
-
 x * 27000 + x * 9000, SDS-PAGE Agromyces sp.
3.5.1.117 9000
-
 x * 27000 + x * 9000, SDS-PAGE Kocuria sp.
3.5.1.117 27000
-
 x * 27000 + x * 9000, SDS-PAGE Agromyces sp.
3.5.1.117 27000
-
 x * 27000 + x * 9000, SDS-PAGE Kocuria sp.

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.46 Agromyces sp.
-
-
-
3.5.1.46 Agromyces sp. KY5R
-
-
-
3.5.1.46 Arthrobacter sp.
-
 encoded by plasmid pOAD2
-
3.5.1.46 Kocuria sp.
-
-
-
3.5.1.46 Kocuria sp. KY2
-
-
-
3.5.1.117 Agromyces sp. Q1EPR5
-
-
3.5.1.117 Agromyces sp. KY5R Q1EPR5
-
-
3.5.1.117 Kocuria sp. Q1EPR4
-
-
3.5.1.117 Kocuria sp. KY2 Q1EPR4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.117
-
 Agromyces sp.
3.5.1.117
-
 Kocuria sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.46 6-aminohexanoate cyclic-oligomer + H2O
-
 Arthrobacter sp. ?
-
 ?
3.5.1.46 6-aminohexanoate cyclic-oligomer + H2O
-
 Agromyces sp. ?
-
 ?
3.5.1.46 6-aminohexanoate cyclic-oligomer + H2O
-
 Kocuria sp. ?
-
 ?
3.5.1.46 6-aminohexanoate cyclic-oligomer + H2O
-
 Kocuria sp. KY2 ?
-
 ?
3.5.1.46 6-aminohexanoate cyclic-oligomer + H2O
-
 Agromyces sp. KY5R ?
-
 ?
3.5.1.117 6-aminohexanoate cyclic oligomer + H2O degree of polymerization > 3. The enzyme produces 6-aminohexanoate from Ahx cyclic oligomers (the NylC-specific substrate) but give no detectable amounts of reaction products from either the 6-aminohexanoate cyclic dimer or the 6-aminohexanoate linear dimer Kocuria sp. 6-aminohexanoate
-
 ?
3.5.1.117 6-aminohexanoate cyclic oligomer + H2O degree of polymerization > 3. The enzyme produces 6-aminohexanoate from Ahx cyclic oligomers (the NylC-specific substrate) but give no detectable amounts of reaction products from either the 6-aminohexanoate cyclic dimer or the 6-aminohexanoate linear dimer Kocuria sp. KY2 6-aminohexanoate
-
 ?
3.5.1.117 6-aminohexanoate cyclic oligomer + H2O degree of polymerization > 3. The enzyme produces 6-aminohexanoate from Ahx cyclic oligomers (the NylC-specific substrate) but give no detectable amounts of reaction products from either the 6-aminohexanoate cyclic dimer or the 6-aminohexanoate linear dimer Agromyces sp. ?
-
 ?
3.5.1.117 6-aminohexanoate cyclic oligomer + H2O degree of polymerization > 3. The enzyme produces 6-aminohexanoate from Ahx cyclic oligomers (the NylC-specific substrate) but give no detectable amounts of reaction products from either the 6-aminohexanoate cyclic dimer or the 6-aminohexanoate linear dimer Agromyces sp. KY5R ?
-
 ?
3.5.1.117 nylon oligomer + H2O
-
 Agromyces sp. ?
-
 ?
3.5.1.117 nylon oligomer + H2O
-
 Kocuria sp. ?
-
 ?
3.5.1.117 nylon oligomer + H2O
-
 Kocuria sp. KY2 ?
-
 ?
3.5.1.117 nylon oligomer + H2O
-
 Agromyces sp. KY5R ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.117 ? x * 27000 + x * 9000, SDS-PAGE Agromyces sp.
3.5.1.117 ? x * 27000 + x * 9000, SDS-PAGE Kocuria sp.

Synonyms

EC Number Synonyms Comment Organism
3.5.1.46 6-aminohexanoate oligomer hydrolase
-
 Arthrobacter sp.
3.5.1.46 6-aminohexanoate oligomer hydrolase
-
 Agromyces sp.
3.5.1.46 6-aminohexanoate oligomer hydrolase
-
 Kocuria sp.
3.5.1.46 NylCA
-
 Agromyces sp.
3.5.1.46 NylCK
-
 Kocuria sp.
3.5.1.46 NylCP2
-
 Arthrobacter sp.
3.5.1.117 Ahx endo-type-oligomer hydrolase
-
 Agromyces sp.
3.5.1.117 Ahx endo-type-oligomer hydrolase
-
 Kocuria sp.
3.5.1.117 NylC
-
 Agromyces sp.
3.5.1.117 NylC
-
 Kocuria sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.117 60
-
 assay at Agromyces sp.
3.5.1.117 60
-
 assay at Kocuria sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.46 40
-
 30 min, stable up to Arthrobacter sp.
3.5.1.46 55
-
 30 min, enzyme retains 90% of its activity Agromyces sp.
3.5.1.46 60
-
 30 min, enzyme retains 90% of its activity Kocuria sp.
3.5.1.117 55
-
 30 min, enzyme retains 90% of its activity Agromyces sp.
3.5.1.117 60
-
 30 min, enzyme retains 90% of its activity Kocuria sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.46 7
-
-
 Arthrobacter sp.
3.5.1.46 7.5
-
-
 Agromyces sp.
3.5.1.46 8
-
-
 Kocuria sp.
3.5.1.117 7.3
-
 assay at Agromyces sp.
3.5.1.117 7.3
-
 assay at Kocuria sp.
3.5.1.117 7.5
-
-
 Agromyces sp.
3.5.1.117 8
-
-
 Kocuria sp.