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Literature summary extracted from

  • Daniel, G.; Volc, J.; Filonova, L.; Plihal, O.; Kubatova, E.; Halada, P.
    Characteristics of Gloeophyllum trabeum alcohol oxidase, an extracellular source of H2O2 in brown rot decay of wood (2007), Appl. Environ. Microbiol., 73, 6241-6253.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.3.13
-
 Gloeophyllum trabeum

General Stability

EC Number General Stability Organism
1.1.3.13 rapid freezing at -80°C and thawing has no significant effect on the enzyme’s activity Gloeophyllum trabeum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.13 2.3
-
 methanol
-
 Gloeophyllum trabeum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.3.13 cell wall the enzyme is localized in the hyphal periplasmic space and wall and on extracellular tripartite membranes and slime, while there is no labeling of hyphal peroxisomes. AOX is associated with membranous or slime structures secreted by hyphae in wood fiber lumina and within the secondary cell walls of degraded wood fibers Gloeophyllum trabeum 5618
-
1.1.3.13 extracellular the enzyme is localized in the hyphal periplasmic space and wall and on extracellular tripartite membranes and slime, while there is no labeling of hyphal peroxisomes. AOX is associated with membranous or slime structures secreted by hyphae in wood fiber lumina and within the secondary cell walls of degraded wood fibers Gloeophyllum trabeum
-
-
1.1.3.13 periplasmic space the enzyme is localized in the hyphal periplasmic space and wall and on extracellular tripartite membranes and slime, while there is no labeling of hyphal peroxisomes. AOX is associated with membranous or slime structures secreted by hyphae in wood fiber lumina and within the secondary cell walls of degraded wood fibers Gloeophyllum trabeum 42597
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.3.13 72400
-
 8 * 72400, SDS-PAGE Gloeophyllum trabeum
1.1.3.13 628000
-
 equilibrium ultracentrifugation Gloeophyllum trabeum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.3.13 methanol + O2 Gloeophyllum trabeum possible role for AOX as a major source of H2O2 formaldehyde + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.13 Gloeophyllum trabeum A8DPS4
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.1.3.13 no glycoprotein
-
 Gloeophyllum trabeum

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.13
-
 Gloeophyllum trabeum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.3.13 hypha the enzyme is localized in the hyphal periplasmic space and wall and on extracellular tripartite membranes and slime, while there is no labeling of hyphal peroxisomes. AOX is associated with membranous or slime structures secreted by hyphae in wood fiber lumina and within the secondary cell walls of degraded wood fibers Gloeophyllum trabeum
-
1.1.3.13 mycelium
-
 Gloeophyllum trabeum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.3.13 12
-
-
 Gloeophyllum trabeum

Storage Stability

EC Number Storage Stability Organism
1.1.3.13 4°C, 1 week storage of the sterile-filtered purified enzyme in 20 mM sodium phosphate (pH 7.5) results in a 68% loss of activity Gloeophyllum trabeum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.13 1-butanol + O2 54% of the activity with methanol Gloeophyllum trabeum n-butanal + H2O2
-
 ?
1.1.3.13 1-pentanol + O2 22% of the activity with methanol Gloeophyllum trabeum n-pentanal + H2O2
-
 ?
1.1.3.13 1-propanol + O2 73% of the activity with methanol Gloeophyllum trabeum n-propanal + H2O2
-
 ?
1.1.3.13 2-methyl-1-propanol + O2 7% of the activity with methanol Gloeophyllum trabeum butan-2-one + H2O2
-
 ?
1.1.3.13 3-methyl-1-butanol + O2 7% of the activity with methanol Gloeophyllum trabeum 3-methylbutanal + H2O2
-
 ?
1.1.3.13 allylalcohol + O2 93% of the activity with methanol Gloeophyllum trabeum acrolein + H2O2
-
 ?
1.1.3.13 benzyl alcohol + O2 9% of the activity with methanol Gloeophyllum trabeum benzaldehyde + H2O2
-
 ?
1.1.3.13 ethanol + O2 94% of the activity with methanol Gloeophyllum trabeum acetaldehyde + H2O2
-
 ?
1.1.3.13 methanol + O2
-
 Gloeophyllum trabeum formaldehyde + H2O2
-
 ?
1.1.3.13 methanol + O2 possible role for AOX as a major source of H2O2 Gloeophyllum trabeum formaldehyde + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.3.13 octamer 8 * 72400, SDS-PAGE Gloeophyllum trabeum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.3.13 15.6
-
 methanol
-
 Gloeophyllum trabeum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.13 6 10 broad optimum Gloeophyllum trabeum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.3.13 additional information
-
 no activity detectable at pH 5.0 and at pH 12.0 Gloeophyllum trabeum
1.1.3.13 5.5
-
 16% of maximal activity Gloeophyllum trabeum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.3.13 7 11 pH stability optimum on the alkaline side in the range of pH 7 to 11. 80% of maximum stability at pH 9 Gloeophyllum trabeum

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.13 FAD noncovalently bonded Gloeophyllum trabeum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.1.3.13 Gloeophyllum trabeum isoelectric focusing
-
 5.3