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Literature summary extracted from

  • Torres-Bacete, J.; Hormigo, D.; Stuart, M.; Arroyo, M.; Torres, P.; Castillon, M.P.; Acebal, C.; Garcia, J.L.; de la Mata, I.
    Newly discovered penicillin acylase activity of aculeacin A acylase from Actinoplanes utahensis (2007), Appl. Environ. Microbiol., 73, 5378-5381.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.5.1.11 synthesis AuAAC should be considered an industrial biocatalyst with high potential in the production of semisynthetic penicillins Actinoplanes utahensis
3.5.1.70 synthesis AuAAC should be considered an industrial biocatalyst with high potential in the production of semisynthetic penicillins Actinoplanes utahensis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.11 expression in Streptomyces lividans Actinoplanes utahensis
3.5.1.70 expression in Streptomyces lividans Actinoplanes utahensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.11 1
-
 penicillin K pH 8.0, 45°C Actinoplanes utahensis
3.5.1.11 5.6
-
 penicillin dihydroF pH 8.0, 45°C Actinoplanes utahensis
3.5.1.11 15.1
-
 penicillin F pH 8.0, 45°C Actinoplanes utahensis
3.5.1.11 15.4
-
 Penicillin V pH 8.0, 45°C Actinoplanes utahensis
3.5.1.11 155.8
-
 penicillin G pH 8.0, 45°C Actinoplanes utahensis
3.5.1.70 1
-
 penicillin K pH 8.0, 45°C Actinoplanes utahensis
3.5.1.70 5.6
-
 penicillin dihydroF pH 8.0, 45°C Actinoplanes utahensis
3.5.1.70 15.1
-
 penicillin F pH 8.0, 45°C Actinoplanes utahensis
3.5.1.70 15.4
-
 Penicillin V pH 8.0, 45°C Actinoplanes utahensis
3.5.1.70 155.8
-
 penicillin G pH 8.0, 45°C Actinoplanes utahensis

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.11 Actinoplanes utahensis
-
-
-
3.5.1.70 Actinoplanes utahensis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.11 recombinant enzyme Actinoplanes utahensis
3.5.1.70 recombinant enzyme Actinoplanes utahensis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.11 42.8
-
-
 Actinoplanes utahensis
3.5.1.70 42.8
-
-
 Actinoplanes utahensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.11 penicillin dihydroF + H2O
-
 Actinoplanes utahensis 6-aminopenicillanic acid + hexanoic acid
-
 ?
3.5.1.11 penicillin F + H2O
-
 Actinoplanes utahensis 6-aminopenicillanic acid + (3E)-hex-3-enoic acid
-
 ?
3.5.1.11 penicillin G + H2O
-
 Actinoplanes utahensis 6-aminopenicillanic acid + phenylacetic acid
-
 ?
3.5.1.11 penicillin K + H2O
-
 Actinoplanes utahensis 6-aminopenicillanic acid + octanoic acid
-
 ?
3.5.1.11 penicillin V + H2O
-
 Actinoplanes utahensis 6-aminopenicillanic acid + phenoxyacetic acid
-
 ?
3.5.1.70 penicillin dihydroF + H2O
-
 Actinoplanes utahensis ?
-
 ?
3.5.1.70 penicillin F + H2O
-
 Actinoplanes utahensis ?
-
 ?
3.5.1.70 penicillin G + H2O
-
 Actinoplanes utahensis 6-aminopenicillanic acid + phenylacetic acid
-
 ?
3.5.1.70 penicillin K + H2O
-
 Actinoplanes utahensis ?
-
 ?
3.5.1.70 penicillin V + H2O
-
 Actinoplanes utahensis 6-aminopenicillanic acid + phenoxyacetic acid
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.1.11 AuAAC
-
 Actinoplanes utahensis
3.5.1.70 AuAAC
-
 Actinoplanes utahensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.11 75
-
-
 Actinoplanes utahensis
3.5.1.70 75
-
-
 Actinoplanes utahensis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.11 50
-
 20 min, stable up to Actinoplanes utahensis
3.5.1.11 82
-
 aculeacin A acylase is highly thermostable, with a midpoint transition temperature of 81.5°C Actinoplanes utahensis
3.5.1.70 50
-
 20 min, stable up to Actinoplanes utahensis
3.5.1.70 82
-
 aculeacin A acylase is highly thermostable, with a midpoint transition temperature of 81.5°C Actinoplanes utahensis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.1.11 1.9
-
 penicillin F pH 8.0, 45°C Actinoplanes utahensis
3.5.1.11 2.2
-
 penicillin G pH 8.0, 45°C Actinoplanes utahensis
3.5.1.11 4.2
-
 penicillin dihydroF pH 8.0, 45°C Actinoplanes utahensis
3.5.1.11 33.3
-
 penicillin K pH 8.0, 45°C Actinoplanes utahensis
3.5.1.11 70.3
-
 Penicillin V pH 8.0, 45°C Actinoplanes utahensis
3.5.1.70 1.9
-
 penicillin F pH 8.0, 45°C Actinoplanes utahensis
3.5.1.70 2.2
-
 penicillin G pH 8.0, 45°C Actinoplanes utahensis
3.5.1.70 4.2
-
 penicillin dihydroF pH 8.0, 45°C Actinoplanes utahensis
3.5.1.70 33.3
-
 penicillin K pH 8.0, 45°C Actinoplanes utahensis
3.5.1.70 70.3
-
 Penicillin V pH 8.0, 45°C Actinoplanes utahensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.11 8 8.5 substrate: penicillin V Actinoplanes utahensis
3.5.1.70 8 8.5 substrate: penicillin V Actinoplanes utahensis