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Literature summary extracted from

  • Nair, N.; Zhao, H.
    Biochemical characterization of an L-Xylulose reductase from Neurospora crassa (2007), Appl. Environ. Microbiol., 73, 2001-2004.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.10 expressed in Escherichia coli as a His6 tag fusion protein Neurospora crassa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.10 0.0026
-
 NADPH
-
 Neurospora crassa
1.1.1.10 0.153
-
 NADP+
-
 Neurospora crassa
1.1.1.10 150
-
 D-ribulose
-
 Neurospora crassa
1.1.1.10 1239
-
 xylitol
-
 Neurospora crassa

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.10 Neurospora crassa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.10 recombinant enzyme Neurospora crassa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.10 D-ribulose + NADPH
-
 Neurospora crassa D-ribitol + NADP+
-
 ?
1.1.1.10 L-xylulose + NADPH + H+
-
 Neurospora crassa L-xylitol + NADP+
-
 r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.10 37
-
 reductase activity Neurospora crassa

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.10 15 55 15°C: about 65% of maximal activity, 55°C: about 60% of maximal activity, reductase activity Neurospora crassa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.10 5.6
-
 xylitol
-
 Neurospora crassa
1.1.1.10 119
-
 D-ribulose
-
 Neurospora crassa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.10 7
-
 reductase activity Neurospora crassa
1.1.1.10 9
-
 oxidase activity Neurospora crassa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.10 4.5 8.5 pH 4.5: about 55% of maximal activity, pH 8.5: about 70% of maximal activity, reductase activity Neurospora crassa
1.1.1.10 7 10.5 pH 7.0: about 40% of maximal activity, pH 10.5: about 60% of maximal activity, oxidase activity Neurospora crassa

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.10 additional information the enzyme does not accept NAD+ Neurospora crassa
1.1.1.10 NADP+
-
 Neurospora crassa