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Literature summary extracted from

  • Stansen, C.; Uy, D.; Delaunay, S.; Eggeling, L.; Goergen, J.-L.; Wendisch, V.F.
    Characterization of a Corynebacterium glutamicum lactate utilization operon induced during temperature-triggered glutamate production (2005), Appl. Environ. Microbiol., 71, 5920-5928.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.5.B3 additional information the enzyme expression is induced by a temperature shift and by growth on L-lactate Corynebacterium glutamicum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.5.B3 gene ldhD, DNA microarray expression analysis, gene ldhD acts in an operon with the permease gene NCgl2816 Corynebacterium glutamicum

Protein Variants

EC Number Protein Variants Comment Organism
1.1.5.B3 additional information disruption of the ldhD-NCgl2816 operon results in loss of the ability to utilize L-lactate as sole carbon source, the ability can be restored by expression of gene ldhD, overview Corynebacterium glutamicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.5.B3 0.51
-
 L-lactate pH 7.5, 30°C Corynebacterium glutamicum

Organism

EC Number Organism UniProt Comment Textmining
1.1.5.B3 Corynebacterium glutamicum
-
 strain ATCC 13032, gene lldD
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.5.B3 0.003
-
 wild-type strain ATCC 13032, without L-lactate Corynebacterium glutamicum
1.1.5.B3 0.23
-
 wild-type strain ATCC 13032, with L-lactate Corynebacterium glutamicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.5.B3 L-lactate + oxidized 2,6-dichlorophenol indophenol stereospecific for L-lactate Corynebacterium glutamicum pyruvate + reduced 2,6-dichlorophenol indophenol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.5.B3 LldD
-
 Corynebacterium glutamicum
1.1.5.B3 quinone-dependent L-lactate dehydrogenase
-
 Corynebacterium glutamicum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.5.B3 30
-
 assay at Corynebacterium glutamicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.5.B3 7.5
-
 assay at Corynebacterium glutamicum

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.5.B3 2,6-dichlorophenol indophenol
-
 Corynebacterium glutamicum
1.1.5.B3 quinone dependent on Corynebacterium glutamicum