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Literature summary extracted from

  • Khatuntseva, S.A.; Eldarov, M.A.; Lopatin, S.A.; Zeinalov, O.A.; Skryabin, K.G.
    Cloning and expression of variants of the glutaryl-7-aminocephalosporic acid acylase of the bacterium Brevundimonas diminuta in Escherichia coli cells (2007), Appl. Biochem. Microbiol., 43, 414-421.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.93 expressed in Escherichia coli BL21 (DE3) cells Brevundimonas diminuta

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.93 Brevundimonas diminuta
-
-
-
3.5.1.93 Brevundimonas diminuta VKM B-1297
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.93 ammonium sulfate precipitation, chitin granula column chromatography, and Co2+-iminodiacetic acid-Sepharose column chromatography Brevundimonas diminuta

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.93 1.6
-
 crude extract, at 37°C Brevundimonas diminuta
3.5.1.93 10
-
 after 6fold purification, at 37°C Brevundimonas diminuta

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.93 cephalosporin C + H2O
-
 Brevundimonas diminuta cephalosporanic acid + 2-amino-5-hydroxypentanoate
-
 ?
3.5.1.93 cephalosporin C + H2O
-
 Brevundimonas diminuta VKM B-1297 cephalosporanic acid + 2-amino-5-hydroxypentanoate
-
 ?
3.5.1.93 glutaryl-7-aminocephalosporanic acid + H2O
-
 Brevundimonas diminuta 7-aminocephalosporanate + glutarate
-
 ?
3.5.1.93 glutaryl-7-aminocephalosporanic acid + H2O
-
 Brevundimonas diminuta VKM B-1297 7-aminocephalosporanate + glutarate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.93 More the enzym is composed of 54 kDa alpha-subunits and 20 kDa beta-subunits Brevundimonas diminuta

Synonyms

EC Number Synonyms Comment Organism
3.5.1.93 Gl7ACA acylase
-
 Brevundimonas diminuta
3.5.1.93 glutaryl-7-aminocephalosporic acid acylase
-
 Brevundimonas diminuta