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Literature summary extracted from

  • Mohamed, S.A.; El-Badry, M.O.; Drees, E.A.; Fahmy, A.S.
    Properties of a cationic peroxidase from Citrus jambhiri cv. Adalia (2008), Appl. Biochem. Biotechnol., 150, 127-137.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.11.1.7 Cu2+ 4% inhibition at 5 mM, at 37°C Citrus jambhiri
1.11.1.7 Hg2+ 80% inhibition at 5 mM, at 37°C Citrus jambhiri
1.11.1.7 Li+ 22% inhibition at 5 mM, at 37°C Citrus jambhiri
1.11.1.7 Ni2+ 10% inhibition at 5 mM, at 37°C Citrus jambhiri
1.11.1.7 Zn2+ 13% inhibition at 5 mM, at 37°C Citrus jambhiri

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.7 0.54
-
 H2O2 in 50 mM sodium acetate buffer, at pH 5.5 Citrus jambhiri
1.11.1.7 2 3 pyrogallol in 50 mM sodium acetate buffer, at pH 5.5 Citrus jambhiri
1.11.1.7 2.85
-
 o-phenylenediamine in 50 mM sodium acetate buffer, at pH 5.5 Citrus jambhiri
1.11.1.7 5
-
 guaiacol in 50 mM sodium acetate buffer, at pH 5.5 Citrus jambhiri
1.11.1.7 11
-
 o-Dianisidine in 50 mM sodium acetate buffer, at pH 5.5 Citrus jambhiri
1.11.1.7 125
-
 catechol in 50 mM sodium acetate buffer, at pH 5.5 Citrus jambhiri

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.11.1.7 Ba2+ 8% increased activity at 5 mM, at 37°C Citrus jambhiri
1.11.1.7 additional information not stimulated by Co2+, Ca2+, Mn2+, and Fe2+ Citrus jambhiri

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.7 56000
-
 SDS-PAGE Citrus jambhiri

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.7 Citrus jambhiri
-
 cultivar Adalia
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.7 ammonium sulfate precipitation, DEAE-cellulose chromatography, carboxymethyl-cellulose column chromatography, and Sephacryl S-200 gel filtration Citrus jambhiri

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.11.1.7 exocarp
-
 Citrus jambhiri
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.11.1.7 additional information
-
 the crude extract has a specific activity of 751 units/mg protein, the 37.6fold purified enzyme has a specific activity of 28261 units/mg protein 1 unit of peroxidase activity is defined as the amount of enzyme that increases the optical density 1.0 per min Citrus jambhiri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.7 catechol + H2O2 4.0% activity compared to guaiacol Citrus jambhiri ?
-
 ?
1.11.1.7 guaiacol + H2O2 100% activity Citrus jambhiri tetraguaiacol + H2O
-
 ?
1.11.1.7 additional information not active towards L-tyrosine, ascorbic acid, alpha-naphthylamine, p-aminoantipyrine, potassium ferrocyanide, potassium iodide, and NADH Citrus jambhiri ?
-
 ?
1.11.1.7 o-dianisidine + H2O2 93.7% activity compared to guaiacol Citrus jambhiri ?
-
 ?
1.11.1.7 o-phenylenediamine + H2O2 171.7% activity compared to guaiacol Citrus jambhiri ?
-
 ?
1.11.1.7 pyrogallol + H2O2 6.2% activity compared to guaiacol Citrus jambhiri purpurogallin + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.11.1.7 cationic peroxidase
-
 Citrus jambhiri
1.11.1.7 POII
-
 Citrus jambhiri

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.11.1.7 40
-
-
 Citrus jambhiri

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.11.1.7 10 70 stable at 10-40°C and unstable above 50°C, 70-80% of POII activity is lost at 50, 60, and 70°C after 15, 10, and 5 min of incubation, respectively Citrus jambhiri

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.7 5.5
-
-
 Citrus jambhiri