EC Number | Application | Comment | Organism |
---|---|---|---|
3.5.1.5 | analysis | urea is estimated in different blood samples with the help of agar-immobilized urease and the results are consistent with those from clinical pathology laboratory through an autoanalyzer | Cajanus cajan |
EC Number | General Stability | Organism |
---|---|---|
3.5.1.5 | agar-immobilized enzyme showes good mechanical stability | Cajanus cajan |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.1.5 | 3.23 | - |
Urea | soluble enzyme | Cajanus cajan | |
3.5.1.5 | 5.07 | - |
Urea | agar-immobilized enzyme | Cajanus cajan |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.1.5 | Cajanus cajan | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.1.5 | urea + H2O | - |
Cajanus cajan | CO2 + NH3 | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.1.5 | 30 | - |
soluble urease | Cajanus cajan |
3.5.1.5 | 60 | - |
agar-immobilized urease | Cajanus cajan |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.1.5 | 20 | 60 | 20°C: about 90% of maximal activity, 60°C: about 40% of maximal activity, soluble enzyme | Cajanus cajan |
3.5.1.5 | 40 | 70 | 40°C: about 50% of maximal activity, 70°C: about 80% of maximal activity, agar-immobilized enzyme | Cajanus cajan |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.1.5 | 4 | - |
pH 7.3, half-life of soluble enzyme is 21 days, half-life of agar-immobilized enzyme is 53 days | Cajanus cajan |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.5.1.5 | 7.3 | - |
soluble enzyme | Cajanus cajan |
3.5.1.5 | 7.5 | - |
agar-immobilized enzyme | Cajanus cajan |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.5.1.5 | 6 | 8.5 | pH 6.0: about 75% of maximal activity, pH 8.5: about 80% of maximal activity, soluble enzyme | Cajanus cajan |
3.5.1.5 | 7 | 8 | pH 7.0: about 65% of maximal activity, pH 8.0: about 70% of maximal activity, agar-immobilized enzyme | Cajanus cajan |