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Literature summary extracted from

  • Mulagalapalli, S.; Kumar, S.; Kalathur, R.C.; Kayastha, A.M.
    Immobilization of urease from pigeonpea (Cajanus cajan) on agar tablets and its application in urea assay (2007), Appl. Biochem. Biotechnol., 142, 291-297.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.5.1.5 analysis urea is estimated in different blood samples with the help of agar-immobilized urease and the results are consistent with those from clinical pathology laboratory through an autoanalyzer Cajanus cajan

General Stability

EC Number General Stability Organism
3.5.1.5 agar-immobilized enzyme showes good mechanical stability Cajanus cajan

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.5 3.23
-
Urea soluble enzyme Cajanus cajan
3.5.1.5 5.07
-
Urea agar-immobilized enzyme Cajanus cajan

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.5 Cajanus cajan
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.5 urea + H2O
-
Cajanus cajan CO2 + NH3
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.5 30
-
soluble urease Cajanus cajan
3.5.1.5 60
-
agar-immobilized urease Cajanus cajan

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.1.5 20 60 20°C: about 90% of maximal activity, 60°C: about 40% of maximal activity, soluble enzyme Cajanus cajan
3.5.1.5 40 70 40°C: about 50% of maximal activity, 70°C: about 80% of maximal activity, agar-immobilized enzyme Cajanus cajan

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.5 4
-
pH 7.3, half-life of soluble enzyme is 21 days, half-life of agar-immobilized enzyme is 53 days Cajanus cajan

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.5 7.3
-
soluble enzyme Cajanus cajan
3.5.1.5 7.5
-
agar-immobilized enzyme Cajanus cajan

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.5 6 8.5 pH 6.0: about 75% of maximal activity, pH 8.5: about 80% of maximal activity, soluble enzyme Cajanus cajan
3.5.1.5 7 8 pH 7.0: about 65% of maximal activity, pH 8.0: about 70% of maximal activity, agar-immobilized enzyme Cajanus cajan