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Literature summary extracted from

  • Tan, Q.; Song, Q.; Zhang, Y.; Wei, D.
    Characterization and application of D-amino acid oxidase and catalase within permeabilized Pichia pastoris cells in bioconversions (2007), Appl. Biochem. Biotechnol., 136, 279-289.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.11.1.6 synthesis high-density fermentation of recombinant Pichia pastoris in a 1-l-fermentor yields 684800 U/l of catalase, measured with permeabilized cells. Catalase shows a half-life of 4 days at 30°C and cells can be reused for synthesis up to 13 times. Permeabilized cells co-expressing catalase and D-amino acid oxidase convert D-phenylalanine into 99% phenylpyruvate within 100 min. In a batchwise conversion of cephalosporin C, about 90% 7-beta-(4-carboxybutanamido)-cephalosporanic acid is obtained at each cycle Komagataella pastoris

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.3 Komagataella pastoris
-
-
-
1.11.1.6 Komagataella pastoris
-
 recombinant enzyme
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.3 cephalosporin C + H2O + O2
-
 Komagataella pastoris 7-(5-oxoadipoamido)cephalosporanic acid + NH3 + H2O2
-
 ?
1.4.3.3 D-alanine + H2O + O2
-
 Komagataella pastoris pyruvate + NH3 + H2O2
-
 ?
1.4.3.3 D-phenylalanine + H2O + O2 conversion of D-phenylalanine to 99.0% phenylpyruvate within 100 min Komagataella pastoris phenylpyruvate + NH3 + H2O2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.4.3.3 D-amino acid oxidase
-
 Komagataella pastoris
1.4.3.3 DAAO
-
 Komagataella pastoris

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.3.3 30 65 the half-life reaches 14.5 d at 30°C Komagataella pastoris

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.4.3.3 11.5
-
 82.6% of DAAO activity is still retained after treatment with base (pH 11.5, 30 min) and heating (65°C, 2 min) Komagataella pastoris

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.3.3 FAD
-
 Komagataella pastoris