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Literature summary extracted from

  • Marchiaro, P.; Tomatis, P.E.; Mussi, M.A.; Pasteran, F.; Viale, A.M; Limansky, A.S.; Vila, A.J.
    Biochemical characterization of metallo-beta-lactamase VIM-11 from a Pseudomonas aeruginosa clinical strain (2007), Antimicrob. Agents Chemother., 52, 2250-2252.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.2.6 expressed in Escherichia coli BL21(DE3) and DH5alpha cells Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.2.6 0.0045
-
 cephalothin
-
 Pseudomonas aeruginosa
3.5.2.6 0.0055
-
 cefuroxime
-
 Pseudomonas aeruginosa
3.5.2.6 0.0074
-
 cefoxitin
-
 Pseudomonas aeruginosa
3.5.2.6 0.0082
-
 nitrocefin
-
 Pseudomonas aeruginosa
3.5.2.6 0.0094
-
 Imipenem
-
 Pseudomonas aeruginosa
3.5.2.6 0.0114
-
 cefotaxime
-
 Pseudomonas aeruginosa
3.5.2.6 0.0153
-
 benzylpenicillin
-
 Pseudomonas aeruginosa
3.5.2.6 0.0227
-
 meropenem
-
 Pseudomonas aeruginosa
3.5.2.6 0.109
-
 ampicillin
-
 Pseudomonas aeruginosa
3.5.2.6 0.11
-
 ceftazidime
-
 Pseudomonas aeruginosa
3.5.2.6 0.183
-
 cefpirome
-
 Pseudomonas aeruginosa
3.5.2.6 1.2
-
 cefepime
-
 Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.6 Pseudomonas aeruginosa
-
-
-
3.5.2.6 Pseudomonas aeruginosa M5109
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.2.6 glutathione-agarose column chromatography Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.6 ampicillin + H2O
-
 Pseudomonas aeruginosa (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 ampicillin + H2O
-
 Pseudomonas aeruginosa M5109 (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 benzylpenicillin + H2O
-
 Pseudomonas aeruginosa (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 benzylpenicillin + H2O
-
 Pseudomonas aeruginosa M5109 (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 cefepime + H2O
-
 Pseudomonas aeruginosa (2R)-2-[(R)-[[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-5-[(1-methylpyrrolidinium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
 ?
3.5.2.6 cefotaxime + H2O
-
 Pseudomonas aeruginosa (2R)-5-[(acetyloxy)methyl]-2-[(R)-[[(2E)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 cefoxitin + H2O
-
 Pseudomonas aeruginosa (2R)-5-[(carbamoyloxy)methyl]-2-[(S)-carboxy(methoxy)[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 cefpirome + H2O
-
 Pseudomonas aeruginosa (2S)-2-[(R)-{[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino}(carboxy)methyl]-5-[(6,7-dihydro-5H-cyclopenta[b]pyridin-1-ium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
 ?
3.5.2.6 ceftazidime + H2O
-
 Pseudomonas aeruginosa (2R)-2-[(R)-{[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-{[(2-carboxypropan-2-yl)oxy]imino}acetyl]amino}(carboxy)methyl]-5-[(pyridin-1-ium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
 ?
3.5.2.6 cefuroxime + H2O
-
 Pseudomonas aeruginosa (2R)-5-[(carbamoyloxy)methyl]-2-[(R)-carboxy{[(2Z)-2-(furan-2-yl)-2-(methoxyimino)acetyl]amino}methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 cefuroxime + H2O
-
 Pseudomonas aeruginosa M5109 (2R)-5-[(carbamoyloxy)methyl]-2-[(R)-carboxy{[(2Z)-2-(furan-2-yl)-2-(methoxyimino)acetyl]amino}methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 cephalothin + H2O
-
 Pseudomonas aeruginosa (2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 cephalothin + H2O
-
 Pseudomonas aeruginosa M5109 (2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 imipenem + H2O
-
 Pseudomonas aeruginosa (5R)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-({2-[(iminomethyl)amino]ethyl}sulfanyl)-4,5-dihydro-1H-pyrrole-2-carboxylic acid
-
 ?
3.5.2.6 meropenem + H2O
-
 Pseudomonas aeruginosa (4R,5S)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-{[(3S,5S)-5-(dimethylcarbamoyl)pyrrolidin-3-yl]sulfanyl}-4-methyl-4,5-dihydro-1H-pyrrole-2-carboxylic acid
-
 ?
3.5.2.6 additional information does not hydrolyse aztreonam Pseudomonas aeruginosa ?
-
 ?
3.5.2.6 additional information does not hydrolyse aztreonam Pseudomonas aeruginosa M5109 ?
-
 ?
3.5.2.6 nitrocefin + H2O
-
 Pseudomonas aeruginosa (2R)-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-5-[(E)-2-(2,4-dinitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.2.6 MbetaL
-
 Pseudomonas aeruginosa
3.5.2.6 metallo-beta-lactamase
-
 Pseudomonas aeruginosa
3.5.2.6 VIM-11
-
 Pseudomonas aeruginosa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.2.6 3.2
-
 meropenem
-
 Pseudomonas aeruginosa
3.5.2.6 6.4
-
 cefoxitin
-
 Pseudomonas aeruginosa
3.5.2.6 14
-
 ceftazidime
-
 Pseudomonas aeruginosa
3.5.2.6 20
-
 Imipenem
-
 Pseudomonas aeruginosa
3.5.2.6 27
-
 cefuroxime
-
 Pseudomonas aeruginosa
3.5.2.6 41.3
-
 cefotaxime
-
 Pseudomonas aeruginosa
3.5.2.6 81
-
 cephalothin
-
 Pseudomonas aeruginosa
3.5.2.6 100
-
 cefepime
-
 Pseudomonas aeruginosa
3.5.2.6 102
-
 cefpirome
-
 Pseudomonas aeruginosa
3.5.2.6 113
-
 benzylpenicillin
-
 Pseudomonas aeruginosa
3.5.2.6 129
-
 nitrocefin
-
 Pseudomonas aeruginosa
3.5.2.6 190
-
 ampicillin
-
 Pseudomonas aeruginosa