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Literature summary extracted from

  • Doi, Y.; Poirel, L.; Paterson, D.L.; Nordmann, P.
    Characterization of a naturally occurring class D beta-lactamase from Achromobacter xylosoxidans (2008), Antimicrob. Agents Chemother., 52, 1952-1956.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.2.6 additional information imipenem and cefoxitin do not induce OXA-114 expression Achromobacter xylosoxidans

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.2.6 expressed in Escherichia coli Top10 cells Achromobacter xylosoxidans

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.2.6 clavulanate
-
 Achromobacter xylosoxidans
3.5.2.6 Sulbactam
-
 Achromobacter xylosoxidans
3.5.2.6 tazobactam
-
 Achromobacter xylosoxidans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.2.6 0.001
-
 piperacillin in 100 mM Tris-H2SO4, 300 mM K2SO4 (pH 7.0), at 30°C Achromobacter xylosoxidans
3.5.2.6 0.01
-
 benzylpenicillin in 100 mM Tris-H2SO4, 300 mM K2SO4 (pH 7.0), at 30°C Achromobacter xylosoxidans
3.5.2.6 0.015
-
 cephalothin in 100 mM Tris-H2SO4, 300 mM K2SO4 (pH 7.0), at 30°C Achromobacter xylosoxidans
3.5.2.6 0.04
-
 ticarcillin in 100 mM Tris-H2SO4, 300 mM K2SO4 (pH 7.0), at 30°C Achromobacter xylosoxidans
3.5.2.6 2
-
 Imipenem Km above 2 mM, in 100 mM Tris-H2SO4, 300 mM K2SO4 (pH 7.0), at 30°C Achromobacter xylosoxidans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.2.6 27000
-
 SDS-PAGE Achromobacter xylosoxidans

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.6 Achromobacter xylosoxidans B3FIE3 strain CIP69598, previously Alcaligenes xylosoxidans
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.2.6 Q-Sepharose column chromatography and S-Sepharose column chromatography Achromobacter xylosoxidans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.6 benzylpenicillin + H2O
-
 Achromobacter xylosoxidans (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 cephalothin + H2O
-
 Achromobacter xylosoxidans (2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 imipenem + H2O
-
 Achromobacter xylosoxidans (5R)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-({2-[(iminomethyl)amino]ethyl}sulfanyl)-4,5-dihydro-1H-pyrrole-2-carboxylic acid
-
 ?
3.5.2.6 additional information no hydrolysis of oxacillin, ceftazidime, cefotaxime, and cefoxitin Achromobacter xylosoxidans ?
-
 ?
3.5.2.6 piperacillin + H2O
-
 Achromobacter xylosoxidans (2R,4S)-2-[(R)-carboxy[[(2R)-2-[[(4-ethyl-2,3-dioxopiperazin-1-yl)carbonyl]amino]-2-phenylacetyl]amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 ticarcillin + H2O
-
 Achromobacter xylosoxidans (2R,4S)-2-[(R)-carboxy[[(2R)-2-carboxy-2-(thiophen-3-yl)acetyl]amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.2.6 OXA114 a class D beta-lactamase Achromobacter xylosoxidans
3.5.2.6 Oxacillinase
-
 Achromobacter xylosoxidans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.2.6 0.1
-
 benzylpenicillin in 100 mM Tris-H2SO4, 300 mM K2SO4 (pH 7.0), at 30°C Achromobacter xylosoxidans
3.5.2.6 0.1
-
 ticarcillin in 100 mM Tris-H2SO4, 300 mM K2SO4 (pH 7.0), at 30°C Achromobacter xylosoxidans
3.5.2.6 0.5
-
 cephalothin in 100 mM Tris-H2SO4, 300 mM K2SO4 (pH 7.0), at 30°C Achromobacter xylosoxidans
3.5.2.6 0.5
-
 piperacillin in 100 mM Tris-H2SO4, 300 mM K2SO4 (pH 7.0), at 30°C Achromobacter xylosoxidans

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.5.2.6 Achromobacter xylosoxidans isoelectric focusing
-
 8.6