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Literature summary extracted from

  • Meziane-Cherif, D.; Lambert, T.; Dupechez, M.; Courvalin, P.; Galimand, M.
    Genetic and biochemical characterization of OXA-63, a new class D beta lactamase from Brachyspira pilosicoli BM4442 (2008), Antimicrob. Agents Chemother., 52, 1264-1268.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.2.6 expressed in Escherichia coli BL21(DE3)pLyS cells Brachyspira pilosicoli

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.2.6 amoxicillin susceptible to amoxicillin in combination with clavulanic acid Brachyspira pilosicoli
3.5.2.6 clavulanic acid strong inhibitor Brachyspira pilosicoli
3.5.2.6 NaCl weak inhibitor Brachyspira pilosicoli
3.5.2.6 Sulbactam
-
 Brachyspira pilosicoli
3.5.2.6 tazobactam strong inhibitor Brachyspira pilosicoli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.2.6 0.017
-
 carbenicillin at 25°C in 100 mM sodium phosphate (pH 7.0) Brachyspira pilosicoli
3.5.2.6 0.019
-
 benzylpenicillin at 25°C in 100 mM sodium phosphate (pH 7.0) Brachyspira pilosicoli
3.5.2.6 0.033
-
 piperacillin at 25°C in 100 mM sodium phosphate (pH 7.0) Brachyspira pilosicoli
3.5.2.6 0.043
-
 ampicillin at 25°C in 100 mM sodium phosphate (pH 7.0) Brachyspira pilosicoli
3.5.2.6 0.115
-
 Oxacillin at 25°C in 100 mM sodium phosphate (pH 7.0) Brachyspira pilosicoli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.2.6 31500
-
 2 * 31500, SDS-PAGE Brachyspira pilosicoli
3.5.2.6 63000
-
 SDS-PAGE Brachyspira pilosicoli

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.6 Brachyspira pilosicoli Q50H31
-
-
3.5.2.6 Brachyspira pilosicoli BM4442 Q50H31
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.2.6 HisTrap Fast Flow column chromatography Brachyspira pilosicoli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.6 ampicillin + H2O
-
 Brachyspira pilosicoli (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 ampicillin + H2O
-
 Brachyspira pilosicoli BM4442 (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 benzylpenicillin + H2O
-
 Brachyspira pilosicoli (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 benzylpenicillin + H2O
-
 Brachyspira pilosicoli BM4442 (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 carbenicillin + H2O
-
 Brachyspira pilosicoli (2R,4S)-2-{(R)-carboxy[2-carboxy(phenyl)acetamido]methyl}-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 carbenicillin + H2O
-
 Brachyspira pilosicoli BM4442 (2R,4S)-2-{(R)-carboxy[2-carboxy(phenyl)acetamido]methyl}-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 additional information does not interact with oxyimino-cephalosporins, imipenem, or aztreonam Brachyspira pilosicoli ?
-
 ?
3.5.2.6 additional information does not interact with oxyimino-cephalosporins, imipenem, or aztreonam Brachyspira pilosicoli BM4442 ?
-
 ?
3.5.2.6 oxacillin + H2O
-
 Brachyspira pilosicoli (2R,4S)-2-{(R)-carboxy[(5-methyl-3-phenyl-1,2-oxazole-4-carbonyl)amino]methyl}-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 oxacillin + H2O
-
 Brachyspira pilosicoli BM4442 (2R,4S)-2-{(R)-carboxy[(5-methyl-3-phenyl-1,2-oxazole-4-carbonyl)amino]methyl}-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 piperacillin + H2O
-
 Brachyspira pilosicoli (2R,4S)-2-[(R)-carboxy[[(2R)-2-[[(4-ethyl-2,3-dioxopiperazin-1-yl)carbonyl]amino]-2-phenylacetyl]amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.2.6 homodimer 2 * 31500, SDS-PAGE Brachyspira pilosicoli

Synonyms

EC Number Synonyms Comment Organism
3.5.2.6 OXA-63
-
 Brachyspira pilosicoli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.2.6 1.1
-
 carbenicillin at 25°C in 100 mM sodium phosphate (pH 7.0) Brachyspira pilosicoli
3.5.2.6 2.1
-
 piperacillin at 25°C in 100 mM sodium phosphate (pH 7.0) Brachyspira pilosicoli
3.5.2.6 4.9
-
 benzylpenicillin at 25°C in 100 mM sodium phosphate (pH 7.0) Brachyspira pilosicoli
3.5.2.6 6.6
-
 ampicillin at 25°C in 100 mM sodium phosphate (pH 7.0) Brachyspira pilosicoli
3.5.2.6 113
-
 Oxacillin at 25°C in 100 mM sodium phosphate (pH 7.0) Brachyspira pilosicoli

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.5.2.6 0.00016
-
 at 25°C in 100 mM sodium phosphate (pH 7.0) Brachyspira pilosicoli tazobactam
3.5.2.6 0.002
-
 at 25°C in 100 mM sodium phosphate (pH 7.0) Brachyspira pilosicoli clavulanic acid
3.5.2.6 0.012
-
 IC50 above 2000 mM, at 25°C in 100 mM sodium phosphate (pH 7.0) Brachyspira pilosicoli Sulbactam
3.5.2.6 2000
-
 IC50 above 2000 mM, at 25°C in 100 mM sodium phosphate (pH 7.0) Brachyspira pilosicoli NaCl