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Literature summary extracted from

  • Fonseca, F.; Sarmento, A.C.; Henriques, I.; Samyn, B.; van Beeumen, J.; Domingues,P.; Domingues, M.R.; Saavedra, M.J.; and Correia, A.
    Biochemical characterization of SFC-1, a class A carbapenem-hydrolyzing beta-lactamase (2007), Antimicrob. Agents Chemother., 51, 4512-4514.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.2.6 expressed in Escherichia coli BL21(DE3)(pMF13) cells Serratia fonticola

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.2.6 clavulanic acid
-
 Serratia fonticola
3.5.2.6 Sulbactam
-
 Serratia fonticola
3.5.2.6 tazobactam
-
 Serratia fonticola

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.2.6 0.026
-
 meropenem at 25°C in 50 mM sodium phosphate (pH 7.0) Serratia fonticola
3.5.2.6 0.038
-
 piperacillin at 25°C in 50 mM sodium phosphate (pH 7.0) Serratia fonticola
3.5.2.6 0.052
-
 ceftazidime at 25°C in 50 mM sodium phosphate (pH 7.0) Serratia fonticola
3.5.2.6 0.077
-
 cefoxitin at 25°C in 50 mM sodium phosphate (pH 7.0) Serratia fonticola
3.5.2.6 0.082
-
 Imipenem at 25°C in 50 mM sodium phosphate (pH 7.0) Serratia fonticola
3.5.2.6 0.089
-
 cefotaxime at 25°C in 50 mM sodium phosphate (pH 7.0) Serratia fonticola
3.5.2.6 0.129
-
 cephalothin at 25°C in 50 mM sodium phosphate (pH 7.0) Serratia fonticola
3.5.2.6 0.176
-
 ampicillin at 25°C in 50 mM sodium phosphate (pH 7.0) Serratia fonticola
3.5.2.6 0.484
-
 aztreonam at 25°C in 50 mM sodium phosphate (pH 7.0) Serratia fonticola

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.2.6 30500
-
 SDS-PAGE Serratia fonticola
3.5.2.6 30770
-
 mature protein, electrospray ionization-mass spectrometry Serratia fonticola
3.5.2.6 31080
-
 electrospray ionization-mass spectrometry Serratia fonticola

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.6 Serratia fonticola
-
-
-
3.5.2.6 Serratia fonticola UTAD54
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.2.6 S-Sepharose column chromatography Serratia fonticola

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.6 a penicillin + H2O
-
 Serratia fonticola a penicilloic acid
-
 ?
3.5.2.6 a penicillin + H2O
-
 Serratia fonticola UTAD54 a penicilloic acid
-
 ?
3.5.2.6 ampicillin + H2O
-
 Serratia fonticola (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 ampicillin + H2O
-
 Serratia fonticola UTAD54 (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 aztreonam + H2O
-
 Serratia fonticola [(1S,2S)-1-[[(2Z)-2-(2-ammonio-1,3-thiazol-4-yl)-2-[[(2-carboxypropan-2-yl)oxy]imino]acetyl]amino]-1-carboxypropan-2-yl]sulfamate
-
 ?
3.5.2.6 aztreonam + H2O
-
 Serratia fonticola UTAD54 [(1S,2S)-1-[[(2Z)-2-(2-ammonio-1,3-thiazol-4-yl)-2-[[(2-carboxypropan-2-yl)oxy]imino]acetyl]amino]-1-carboxypropan-2-yl]sulfamate
-
 ?
3.5.2.6 carbapenem + H2O
-
 Serratia fonticola [(2R)-2,3-dihydro-1H-pyrrol-2-yl]acetic acid
-
 ?
3.5.2.6 cefotaxime + H2O
-
 Serratia fonticola (2R)-5-[(acetyloxy)methyl]-2-[(R)-[[(2E)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 cefotaxime + H2O
-
 Serratia fonticola UTAD54 (2R)-5-[(acetyloxy)methyl]-2-[(R)-[[(2E)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 cefoxitin + H2O
-
 Serratia fonticola (2R)-5-[(carbamoyloxy)methyl]-2-[(S)-carboxy(methoxy)[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 ceftazidime + H2O
-
 Serratia fonticola (2R)-2-[(R)-{[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-{[(2-carboxypropan-2-yl)oxy]imino}acetyl]amino}(carboxy)methyl]-5-[(pyridin-1-ium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
 ?
3.5.2.6 cephalosporin + H2O
-
 Serratia fonticola ?
-
 ?
3.5.2.6 cephalothin + H2O
-
 Serratia fonticola (2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 cephalothin + H2O
-
 Serratia fonticola UTAD54 (2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 imipenem + H2O
-
 Serratia fonticola (5R)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-({2-[(iminomethyl)amino]ethyl}sulfanyl)-4,5-dihydro-1H-pyrrole-2-carboxylic acid
-
 ?
3.5.2.6 meropenem + H2O
-
 Serratia fonticola (4R,5S)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-{[(3S,5S)-5-(dimethylcarbamoyl)pyrrolidin-3-yl]sulfanyl}-4-methyl-4,5-dihydro-1H-pyrrole-2-carboxylic acid
-
 ?
3.5.2.6 piperacillin + H2O
-
 Serratia fonticola (2R,4S)-2-[(R)-carboxy[[(2R)-2-[[(4-ethyl-2,3-dioxopiperazin-1-yl)carbonyl]amino]-2-phenylacetyl]amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.2.6 SFC-1
-
 Serratia fonticola

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.2.6 2.1
-
 ceftazidime at 25°C in 50 mM sodium phosphate (pH 7.0) Serratia fonticola
3.5.2.6 4.2
-
 cefoxitin at 25°C in 50 mM sodium phosphate (pH 7.0) Serratia fonticola
3.5.2.6 6.5
-
 meropenem at 25°C in 50 mM sodium phosphate (pH 7.0) Serratia fonticola
3.5.2.6 8.3
-
 cefotaxime at 25°C in 50 mM sodium phosphate (pH 7.0) Serratia fonticola
3.5.2.6 44.7
-
 piperacillin at 25°C in 50 mM sodium phosphate (pH 7.0) Serratia fonticola
3.5.2.6 54
-
 Imipenem at 25°C in 50 mM sodium phosphate (pH 7.0) Serratia fonticola
3.5.2.6 155
-
 ampicillin at 25°C in 50 mM sodium phosphate (pH 7.0) Serratia fonticola
3.5.2.6 162
-
 aztreonam at 25°C in 50 mM sodium phosphate (pH 7.0) Serratia fonticola
3.5.2.6 280
-
 cephalothin at 25°C in 50 mM sodium phosphate (pH 7.0) Serratia fonticola

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.5.2.6 Serratia fonticola purified SFC-1 migrates as a single band, but isoelectric focusing reveals two forms of purified SFC-1, with pI values of 7.6 and 8.2
-
 7.6
3.5.2.6 Serratia fonticola purified SFC-1 migrates as a single band, but isoelectric focusing reveals two forms of purified SFC-1, with pI values of 7.6 and 8.2
-
 8.2