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Literature summary extracted from

  • Girlich, D. ; Leclercq, R. ; Naas, T.; Nordmanni, P.
    Molecular and biochemical characterization of the chromosome-encoded class A beta-lactamase BCL-1 from Bacillus clausii (2007), Antimicrob. Agents Chemother., 51, 4009-4014.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.2.6 expressed in Escherichia coli BL21(DE3) cells Alkalihalobacillus clausii

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.2.6 clavulanic acid
-
 Alkalihalobacillus clausii
3.5.2.6 Sulbactam
-
 Alkalihalobacillus clausii
3.5.2.6 tazobactam
-
 Alkalihalobacillus clausii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.2.6 0.00001
-
 cefoxitin Km less than 0.00001 mM at 30°C in 100 mM sodium phosphate (pH 7) Alkalihalobacillus clausii
3.5.2.6 0.006
-
 ticarcillin at 30°C in 100 mM sodium phosphate (pH 7) Alkalihalobacillus clausii
3.5.2.6 0.012
-
 benzylpenicillin at 30°C in 100 mM sodium phosphate (pH 7) Alkalihalobacillus clausii
3.5.2.6 0.015
-
 cephalothin at 30°C in 100 mM sodium phosphate (pH 7) Alkalihalobacillus clausii
3.5.2.6 0.021
-
 piperacillin at 30°C in 100 mM sodium phosphate (pH 7) Alkalihalobacillus clausii
3.5.2.6 0.022
-
 amoxicillin at 30°C in 100 mM sodium phosphate (pH 7) Alkalihalobacillus clausii
3.5.2.6 0.035
-
 Imipenem at 30°C in 100 mM sodium phosphate (pH 7) Alkalihalobacillus clausii
3.5.2.6 0.045
-
 cephaloridine at 30°C in 100 mM sodium phosphate (pH 7) Alkalihalobacillus clausii
3.5.2.6 0.105
-
 cefuroxime at 30°C in 100 mM sodium phosphate (pH 7) Alkalihalobacillus clausii
3.5.2.6 0.136
-
 cefpirome at 30°C in 100 mM sodium phosphate (pH 7) Alkalihalobacillus clausii
3.5.2.6 0.79
-
 cefotaxime at 30°C in 100 mM sodium phosphate (pH 7) Alkalihalobacillus clausii
3.5.2.6 0.95
-
 ceftriaxone at 30°C in 100 mM sodium phosphate (pH 7) Alkalihalobacillus clausii
3.5.2.6 1
-
 ceftazidime Km above 1.0 mM at 30°C in 100 mM sodium phosphate (pH 7) Alkalihalobacillus clausii
3.5.2.6 1
-
 cefepime Km above 1.0 mM at 30°C in 100 mM sodium phosphate (pH 7) Alkalihalobacillus clausii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.2.6 32000
-
 SDS-PAGE Alkalihalobacillus clausii

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.6 Alkalihalobacillus clausii A8RR46
-
-
3.5.2.6 Alkalihalobacillus clausii NR A8RR46
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.2.6 S-Sepharose column chromatography and Q-Sepharose column chromatography Alkalihalobacillus clausii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.2.6 additional information
-
 The specific activity of purified BCL-1, measured with 0.1 mM of cephalothin as the substrate, was 35 units/mg of protein with a 26fold purification factor Alkalihalobacillus clausii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.6 amoxicillin + H2O
-
 Alkalihalobacillus clausii (2R,4S)-2-[(R)-{[(2R)-2-amino-2-(4-hydroxyphenyl)acetyl]amino}(carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 aztreonam + H2O
-
 Alkalihalobacillus clausii [(1S,2S)-1-[[(2Z)-2-(2-ammonio-1,3-thiazol-4-yl)-2-[[(2-carboxypropan-2-yl)oxy]imino]acetyl]amino]-1-carboxypropan-2-yl]sulfamate
-
 ?
3.5.2.6 aztreonam + H2O
-
 Alkalihalobacillus clausii NR [(1S,2S)-1-[[(2Z)-2-(2-ammonio-1,3-thiazol-4-yl)-2-[[(2-carboxypropan-2-yl)oxy]imino]acetyl]amino]-1-carboxypropan-2-yl]sulfamate
-
 ?
3.5.2.6 benzylpenicillin + H2O
-
 Alkalihalobacillus clausii (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 benzylpenicillin + H2O
-
 Alkalihalobacillus clausii NR (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 cefepime + H2O
-
 Alkalihalobacillus clausii (2R)-2-[(R)-[[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-5-[(1-methylpyrrolidinium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
 ?
3.5.2.6 cefotaxime + H2O
-
 Alkalihalobacillus clausii (2R)-5-[(acetyloxy)methyl]-2-[(R)-[[(2E)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 cefoxitin + H2O
-
 Alkalihalobacillus clausii (2R)-5-[(carbamoyloxy)methyl]-2-[(S)-carboxy(methoxy)[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 cefpirome + H2O
-
 Alkalihalobacillus clausii (2S)-2-[(R)-{[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino}(carboxy)methyl]-5-[(6,7-dihydro-5H-cyclopenta[b]pyridin-1-ium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
 ?
3.5.2.6 ceftazidime + H2O
-
 Alkalihalobacillus clausii (2R)-2-[(R)-{[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-{[(2-carboxypropan-2-yl)oxy]imino}acetyl]amino}(carboxy)methyl]-5-[(pyridin-1-ium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
 ?
3.5.2.6 ceftriaxone + H2O
-
 Alkalihalobacillus clausii (2R)-2-[(R)-[[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-5-[[(6-hydroxy-2-methyl-5-oxo-2,5-dihydro-1,2,4-triazin-3-yl)sulfanyl]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 cefuroxime + H2O
-
 Alkalihalobacillus clausii (2R)-5-[(carbamoyloxy)methyl]-2-[(R)-carboxy{[(2Z)-2-(furan-2-yl)-2-(methoxyimino)acetyl]amino}methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 cefuroxime + H2O
-
 Alkalihalobacillus clausii NR (2R)-5-[(carbamoyloxy)methyl]-2-[(R)-carboxy{[(2Z)-2-(furan-2-yl)-2-(methoxyimino)acetyl]amino}methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 cephaloridine + H2O
-
 Alkalihalobacillus clausii (2R)-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-5-(pyridinium-1-ylmethyl)-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
 ?
3.5.2.6 cephaloridine + H2O
-
 Alkalihalobacillus clausii NR (2R)-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-5-(pyridinium-1-ylmethyl)-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
 ?
3.5.2.6 cephalothin + H2O
-
 Alkalihalobacillus clausii (2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 cephalothin + H2O
-
 Alkalihalobacillus clausii NR (2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 imipenem + H2O
-
 Alkalihalobacillus clausii (5R)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-({2-[(iminomethyl)amino]ethyl}sulfanyl)-4,5-dihydro-1H-pyrrole-2-carboxylic acid
-
 ?
3.5.2.6 moxalactam + H2O
-
 Alkalihalobacillus clausii (2R)-2-{(R)-carboxy[2-carboxy(4-hydroxyphenyl)acetamido]methoxymethyl}-5-{[(1-methyl-1H-tetrazol-5-yl)sulfanyl]methyl}-3,6-dihydro-2H-1,3-oxazine-4-carboxylic acid
-
 ?
3.5.2.6 piperacillin + H2O
-
 Alkalihalobacillus clausii (2R,4S)-2-[(R)-carboxy[[(2R)-2-[[(4-ethyl-2,3-dioxopiperazin-1-yl)carbonyl]amino]-2-phenylacetyl]amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 ticarcillin + H2O
-
 Alkalihalobacillus clausii (2R,4S)-2-[(R)-carboxy[[(2R)-2-carboxy-2-(thiophen-3-yl)acetyl]amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.2.6 BCL-1
-
 Alkalihalobacillus clausii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.2.6 0.01
-
 cefoxitin kcat less than 0.01/sec at 30°C in 100 mM sodium phosphate (pH 7) Alkalihalobacillus clausii
3.5.2.6 0.01
-
 moxalactam kcat less than 0.01/sec at 30°C in 100 mM sodium phosphate (pH 7) Alkalihalobacillus clausii
3.5.2.6 0.06
-
 Imipenem at 30°C in 100 mM sodium phosphate (pH 7) Alkalihalobacillus clausii
3.5.2.6 0.3
-
 aztreonam kcat less than 0.3/sec at 30°C in 100 mM sodium phosphate (pH 7) Alkalihalobacillus clausii
3.5.2.6 3.3
-
 ceftazidime kcat above than 3.3/sec at 30°C in 100 mM sodium phosphate (pH 7) Alkalihalobacillus clausii
3.5.2.6 5
-
 ticarcillin at 30°C in 100 mM sodium phosphate (pH 7) Alkalihalobacillus clausii
3.5.2.6 15
-
 cefuroxime at 30°C in 100 mM sodium phosphate (pH 7) Alkalihalobacillus clausii
3.5.2.6 22
-
 cephalothin at 30°C in 100 mM sodium phosphate (pH 7) Alkalihalobacillus clausii
3.5.2.6 22
-
 cefotaxime at 30°C in 100 mM sodium phosphate (pH 7) Alkalihalobacillus clausii
3.5.2.6 30
-
 ceftriaxone at 30°C in 100 mM sodium phosphate (pH 7) Alkalihalobacillus clausii
3.5.2.6 38
-
 piperacillin at 30°C in 100 mM sodium phosphate (pH 7) Alkalihalobacillus clausii
3.5.2.6 40
-
 cefepime kcat above 40/sec at 30°C in 100 mM sodium phosphate (pH 7) Alkalihalobacillus clausii
3.5.2.6 85
-
 amoxicillin at 30°C in 100 mM sodium phosphate (pH 7) Alkalihalobacillus clausii
3.5.2.6 95
-
 cefpirome at 30°C in 100 mM sodium phosphate (pH 7) Alkalihalobacillus clausii
3.5.2.6 97
-
 benzylpenicillin at 30°C in 100 mM sodium phosphate (pH 7) Alkalihalobacillus clausii
3.5.2.6 115
-
 cephaloridine at 30°C in 100 mM sodium phosphate (pH 7) Alkalihalobacillus clausii

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.5.2.6 Alkalihalobacillus clausii isoelectric focusing
-
 5.5

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.5.2.6 0.000004
-
 using cephalothin as a substrate, at 30°C Alkalihalobacillus clausii tazobactam
3.5.2.6 0.000075
-
 using cephalothin as a substrate, at 30°C Alkalihalobacillus clausii Sulbactam
3.5.2.6 0.000085
-
 using cephalothin as a substrate, at 30°C Alkalihalobacillus clausii clavulanic acid