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Literature summary extracted from

  • Perilli, M.; Caporale, B.; Celenza, G.; Pellegrini, C.; Docquier, J.D.; Mezzatesta, M.; Rossolini, G.M.; Stefani, S.; Amicosante, G.
    Identification and characterization of a new metallo-beta-lactamase, IND-5, from a clinical isolate of Chryseobacterium indologenes (2006), Antimicrob. Agents Chemother., 51, 2988-2990.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.2.6 expressed in Escherichia coli BL21(DE3) cells Chryseobacterium indologenes

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.2.6 1,10-ophenanthroline
-
Chryseobacterium indologenes
3.5.2.6 dipicolinic acid
-
Chryseobacterium indologenes
3.5.2.6 EDTA
-
Chryseobacterium indologenes

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.2.6 0.008
-
nitrocefin in 30 mM sodium cacodylate buffer (pH 6.5) plus 0.05 mM ZnSO4 Chryseobacterium indologenes
3.5.2.6 0.013
-
benzylpenicillin in 30 mM sodium cacodylate buffer (pH 6.5) plus 0.05 mM ZnSO4 Chryseobacterium indologenes
3.5.2.6 0.025
-
cefazolin in 30 mM sodium cacodylate buffer (pH 6.5) plus 0.05 mM ZnSO4 Chryseobacterium indologenes
3.5.2.6 0.049
-
cefotaxime in 30 mM sodium cacodylate buffer (pH 6.5) plus 0.05 mM ZnSO4 Chryseobacterium indologenes
3.5.2.6 0.15
-
ampicillin in 30 mM sodium cacodylate buffer (pH 6.5) plus 0.05 mM ZnSO4 Chryseobacterium indologenes
3.5.2.6 0.28
-
meropenem in 30 mM sodium cacodylate buffer (pH 6.5) plus 0.05 mM ZnSO4 Chryseobacterium indologenes
3.5.2.6 0.3
-
Imipenem in 30 mM sodium cacodylate buffer (pH 6.5) plus 0.05 mM ZnSO4 Chryseobacterium indologenes
3.5.2.6 0.32
-
carbenicillin in 30 mM sodium cacodylate buffer (pH 6.5) plus 0.05 mM ZnSO4 Chryseobacterium indologenes
3.5.2.6 0.375
-
piperacillin in 30 mM sodium cacodylate buffer (pH 6.5) plus 0.05 mM ZnSO4 Chryseobacterium indologenes
3.5.2.6 1.3
-
Sulbactam in 30 mM sodium cacodylate buffer (pH 6.5) plus 0.05 mM ZnSO4 Chryseobacterium indologenes

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.2.6 24540
-
calculated from amino acid sequence Chryseobacterium indologenes
3.5.2.6 25000
-
SDS-PAGE Chryseobacterium indologenes

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.6 Chryseobacterium indologenes Q6JE29
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.2.6 Mono Q Sepharose column chromatography Chryseobacterium indologenes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.6 ampicillin + H2O
-
Chryseobacterium indologenes (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
?
3.5.2.6 benzylpenicillin + H2O
-
Chryseobacterium indologenes (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
?
3.5.2.6 carbenicillin + H2O
-
Chryseobacterium indologenes (2R,4S)-2-{(R)-carboxy[2-carboxy(phenyl)acetamido]methyl}-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
?
3.5.2.6 cefazolin + H2O
-
Chryseobacterium indologenes (2R)-2-[(R)-carboxy[(1H-tetrazol-1-ylacetyl)amino]methyl]-5-[[(5-methyl-1,3,4-thiadiazol-2-yl)sulfanyl]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
?
3.5.2.6 cefotaxime + H2O
-
Chryseobacterium indologenes (2R)-5-[(acetyloxy)methyl]-2-[(R)-[[(2E)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
?
3.5.2.6 imipenem + H2O
-
Chryseobacterium indologenes (5R)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-({2-[(iminomethyl)amino]ethyl}sulfanyl)-4,5-dihydro-1H-pyrrole-2-carboxylic acid
-
?
3.5.2.6 meropenem + H2O
-
Chryseobacterium indologenes (4R,5S)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-{[(3S,5S)-5-(dimethylcarbamoyl)pyrrolidin-3-yl]sulfanyl}-4-methyl-4,5-dihydro-1H-pyrrole-2-carboxylic acid
-
?
3.5.2.6 additional information no hydrolysis of ceftazidime, cefepime, moxalactam, tazobactam, and aztreonam Chryseobacterium indologenes ?
-
?
3.5.2.6 nitrocefin + H2O
-
Chryseobacterium indologenes (2R)-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-5-[(E)-2-(2,4-dinitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
?
3.5.2.6 piperacillin + H2O
-
Chryseobacterium indologenes (2R,4S)-2-[(R)-carboxy[[(2R)-2-[[(4-ethyl-2,3-dioxopiperazin-1-yl)carbonyl]amino]-2-phenylacetyl]amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
?
3.5.2.6 sulbactam + H2O
-
Chryseobacterium indologenes (2R,4S)-2-(carboxymethyl)-5,5-dimethyl-1,1-dioxo-1lambda~6~,3-thiazolidine-4-carboxylic acid
-
?

Synonyms

EC Number Synonyms Comment Organism
3.5.2.6 IND-5
-
Chryseobacterium indologenes
3.5.2.6 MBL
-
Chryseobacterium indologenes
3.5.2.6 metallo-beta-lactamase
-
Chryseobacterium indologenes

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.2.6 40 55 the enzyme exhibits a half-life of 90 min at 40°C, 25 min at 45°C, and less than 1 min at 55°C Chryseobacterium indologenes

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.2.6 0.46
-
cefazolin in 30 mM sodium cacodylate buffer (pH 6.5) plus 0.05 mM ZnSO4 Chryseobacterium indologenes
3.5.2.6 1.53
-
cefotaxime in 30 mM sodium cacodylate buffer (pH 6.5) plus 0.05 mM ZnSO4 Chryseobacterium indologenes
3.5.2.6 3.3
-
Sulbactam in 30 mM sodium cacodylate buffer (pH 6.5) plus 0.05 mM ZnSO4 Chryseobacterium indologenes
3.5.2.6 4.13
-
meropenem in 30 mM sodium cacodylate buffer (pH 6.5) plus 0.05 mM ZnSO4 Chryseobacterium indologenes
3.5.2.6 4.4
-
nitrocefin in 30 mM sodium cacodylate buffer (pH 6.5) plus 0.05 mM ZnSO4 Chryseobacterium indologenes
3.5.2.6 7.28
-
Imipenem in 30 mM sodium cacodylate buffer (pH 6.5) plus 0.05 mM ZnSO4 Chryseobacterium indologenes
3.5.2.6 10.3
-
ampicillin in 30 mM sodium cacodylate buffer (pH 6.5) plus 0.05 mM ZnSO4 Chryseobacterium indologenes
3.5.2.6 10.92
-
benzylpenicillin in 30 mM sodium cacodylate buffer (pH 6.5) plus 0.05 mM ZnSO4 Chryseobacterium indologenes
3.5.2.6 14.61
-
piperacillin in 30 mM sodium cacodylate buffer (pH 6.5) plus 0.05 mM ZnSO4 Chryseobacterium indologenes
3.5.2.6 16.96
-
carbenicillin in 30 mM sodium cacodylate buffer (pH 6.5) plus 0.05 mM ZnSO4 Chryseobacterium indologenes

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.5.2.6 0.003
-
dipicolinic acid
-
Chryseobacterium indologenes
3.5.2.6 0.0073
-
1,10-ophenanthroline
-
Chryseobacterium indologenes
3.5.2.6 0.815
-
EDTA
-
Chryseobacterium indologenes

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.5.2.6 Chryseobacterium indologenes isoelectric focusing
-
8.8