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Literature summary extracted from

  • Horsfall, L.E.; Garau, G.; Lienard, B.M.; Dideberg, O.; Schofield, C.J.; Frere, J.M.; Galleni, M.
    Competitive inhibitors of the CphA metallo-beta-lactamase from Aeromonas hydrophila (2007), Antimicrob. Agents Chemother., 51, 2136-2142.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.2.6 pyridine-2,3-dicarboxylic acid 30% increase of activity at 0.1 mM Aeromonas hydrophila
3.5.2.6 pyridine-2,5-dicarboxylic acid 14% increase of activity at 0.1 mM Aeromonas hydrophila
3.5.2.6 pyridine-3,4-dicarboxylic acid 16% increase of activity at 0.1 mM Aeromonas hydrophila

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.5.2.6 hanging drop vapour diffusion method with 30% (w/v) polyethylene glycol 8000, 0.6 M ammonium sulfate, and 100 mM Na-citrate, pH 6.5 Aeromonas hydrophila

Protein Variants

EC Number Protein Variants Comment Organism
3.5.2.6 N116H/N220G mutant enzyme with a broader specificity than wild type CphA Aeromonas hydrophila

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.2.6 2-picolinic acid competitive inhibitor, 8% residual activity at 0.1 mM Aeromonas hydrophila
3.5.2.6 pyridine-2,4-dicarboxylic acid competitive inhibitor, 38% residual activity at 0.1 mM Aeromonas hydrophila
3.5.2.6 pyridine-2,6-dicarboxylic acid 12% residual activity at 0.1 mM Aeromonas hydrophila

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.2.6 0.063
-
 Imipenem wild type enzyme, at pH 9.0 Aeromonas hydrophila
3.5.2.6 0.083
-
 Imipenem wild type enzyme, at pH 8.0 Aeromonas hydrophila
3.5.2.6 0.12
-
 Imipenem wild type enzyme, at pH 7.0 Aeromonas hydrophila

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.2.6 Zn2+ catalytically active with one zinc Aeromonas hydrophila

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.6 Aeromonas hydrophila P26918
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.6 ampicillin + H2O
-
 Aeromonas hydrophila (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 imipenem + H2O
-
 Aeromonas hydrophila (5R)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-({2-[(iminomethyl)amino]ethyl}sulfanyl)-4,5-dihydro-1H-pyrrole-2-carboxylic acid
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.2.6 CphA
-
 Aeromonas hydrophila
3.5.2.6 metallo-beta-lactamase
-
 Aeromonas hydrophila

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.2.6 49
-
 Imipenem wild type enzyme, at pH 9.0 Aeromonas hydrophila
3.5.2.6 140
-
 Imipenem wild type enzyme, at pH 8.0 Aeromonas hydrophila
3.5.2.6 210
-
 Imipenem wild type enzyme, at pH 7.0 Aeromonas hydrophila

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.5.2.6 0.0045
-
 pyridine-2,4-dicarboxylic acid wild type enzyme, in 20 mM sodium cacodylate buffer, pH 6.5 Aeromonas hydrophila
3.5.2.6 0.0057
-
 2-picolinic acid wild type enzyme, in 20 mM sodium cacodylate buffer, pH 6.5 Aeromonas hydrophila
3.5.2.6 0.013
-
 2-picolinic acid mutant enzyme N116H/N220G, in 20 mM sodium cacodylate buffer, pH 6.5 Aeromonas hydrophila
3.5.2.6 0.028
-
 pyridine-2,4-dicarboxylic acid mutant enzyme N116H/N220G, in 20 mM sodium cacodylate buffer, pH 6.5 Aeromonas hydrophila