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Literature summary extracted from

  • Nazi, I.; Scott, A.; Sham, A.; Rossi, L.; Williamson, P.R.; Kronstad, J.W.; Wright, G.D.
    Role of homoserine transacetylase as a new target for antifungal agents (2007), Antimicrob. Agents Chemother., 51, 1731-1736.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.3.1.31 medicine using a mouse inhalation model of infection it is shown that MET2 is required for virulence, making fungal HTA a viable target for new antibiotic discovery Cryptococcus neoformans

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.31 expressed in Escherichia coli as a His-tagged fusion protein Cryptococcus neoformans

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.31 additional information Met auxotrophy is shown by a constructed MET2 mutant and its growth behavior in Met-deficient media Cryptococcus neoformans

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.31 6-carbamoyl-3a,4,5,9b-tetrahydro-3H-cyclopenta[c]quinoline-4-carboxylic acid competitive inhibitor of acetyl-CoA Cryptococcus neoformans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.31 additional information
-
additional information kcat/Km (substrate L-homoserine): 130000/Msec, (substrate acetyl-CoA): 878000/Msec Cryptococcus neoformans

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.31 Cryptococcus neoformans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.31 using Ni-NTA chromatography Cryptococcus neoformans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.31 acetyl-CoA + L-homoserine
-
Cryptococcus neoformans CoA + O-acetyl-L-homoserine
-
?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.31 CnHTA
-
Cryptococcus neoformans
2.3.1.31 homoserine transacetylase
-
Cryptococcus neoformans
2.3.1.31 MET2
-
Cryptococcus neoformans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.31 122
-
L-homoserine
-
Cryptococcus neoformans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.31 8
-
assay at Cryptococcus neoformans

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.1.31 0.0136
-
6-carbamoyl-3a,4,5,9b-tetrahydro-3H-cyclopenta[c]quinoline-4-carboxylic acid Ki value of competitive inhibition of acetyl-CoA Cryptococcus neoformans
2.3.1.31 0.0917
-
6-carbamoyl-3a,4,5,9b-tetrahydro-3H-cyclopenta[c]quinoline-4-carboxylic acid Ki value of noncompetitive inhibition of L-homoserine Cryptococcus neoformans

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.3.1.31 0.156
-
in the presence of 0.01 mM acetyl-CoA Cryptococcus neoformans 6-carbamoyl-3a,4,5,9b-tetrahydro-3H-cyclopenta[c]quinoline-4-carboxylic acid
2.3.1.31 0.287
-
in the presence of 0.1 mM acetyl-CoA Cryptococcus neoformans 6-carbamoyl-3a,4,5,9b-tetrahydro-3H-cyclopenta[c]quinoline-4-carboxylic acid