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Literature summary extracted from
- Glutaraldehyde activated eggshell membrane for immobilization of tyrosinase from Amorphophallus companulatus: application in construction of electrochemical biosensor for dopamine (2008), Anal. Chim. Acta, 612, 212-217.
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.18.1 | 0.2 | - |
dopamine | soluble enzyme | Amorphophallus paeoniifolius var. campanulatus |  |
| 1.14.18.1 | 0.67 | - |
dopamine | using tyrosinase immobilized on eggshell membrane. Km is 0.67 mM and Vmax 0.08 mM min-1. Increase in Km (compared to soluble enzyme for which Km is 0.20 mM) indicate that the affinity of enzyme for substrate has decreased to some extent after immobilization, which may be due to glutaraldehyde treatment | Amorphophallus paeoniifolius var. campanulatus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.18.1 | Amorphophallus paeoniifolius var. campanulatus | - |
- |
- |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.14.18.1 | Shelf life of immobilized tyrosinase system is found to be more than 6 months when stored in phosphate buffer at 4°C. Immobilization on preactivated eggshell membrane followed by enzyme adsorption is a simple and a fast procedure to prepare stable immobilized tyrosinase. | Amorphophallus paeoniifolius var. campanulatus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.18.1 | dopamine + 1/2 O2 | - |
Amorphophallus paeoniifolius var. campanulatus | dopamine quinone + H2O | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.18.1 | polyphenol oxidase | a binuclear copper containing metalloprotein | Amorphophallus paeoniifolius var. campanulatus |
| 1.14.18.1 | tyrosinase | - |
Amorphophallus paeoniifolius var. campanulatus |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.18.1 | 20 | 45 | membrane bound enzyme exhibits consistent activity in the temperature range 20-45°C | Amorphophallus paeoniifolius var. campanulatus |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.18.1 | 5 | 6.5 | studies on effect of pH show retention of more than 90% activity over a pH range 5.0-6.5 | Amorphophallus paeoniifolius var. campanulatus |
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