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Literature summary extracted from
- High-throughput screening assay for D-amino acid oxidase (2008), Anal. Biochem., 374, 405-410.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 1.4.3.3 | biotechnology | DAAO can be used to synthesize cephalosporin antibiotics | Trigonopsis variabilis |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.4.3.3 | expressed in Escherichia coli | Trigonopsis variabilis |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.4.3.3 | Trigonopsis variabilis | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.4.3.3 | Mono Q HR 10/10 column chromatography and Sephacryl S-200 gel filtration | Trigonopsis variabilis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.4.3.3 | D-3,4-dihydroxyphenylalanine + H2O + O2 | - |
Trigonopsis variabilis | ? | - |
? |  |
| 1.4.3.3 | D-alanine + H2O + O2 | highly active | Trigonopsis variabilis | pyruvate + NH3 + H2O2 | - |
? | |
| 1.4.3.3 | D-lysine + H2O + O2 | poor substrate | Trigonopsis variabilis | 6-amino-2-oxohexanoic acid + NH3 + H2O2 | - |
? | |
| 1.4.3.3 | D-methionine + H2O + O2 | highly active | Trigonopsis variabilis | 4-methylthio-2-oxobutanoate + NH3 + H2O2 | - |
? | |
| 1.4.3.3 | D-serine + H2O + O2 | poor substrate | Trigonopsis variabilis | 2-oxo-3-hydroxypropionate + NH3 + H2O2 | - |
? | |
| 1.4.3.3 | D-threonine + H2O + O2 | poor substrate | Trigonopsis variabilis | 2-oxo-3-hydroxybutyrate + NH3 + H2O2 | - |
? | |
| 1.4.3.3 | D-valine + H2O + O2 | highly active | Trigonopsis variabilis | alpha-ketoisovalerate + NH3 + H2O2 | - |
? | |
| 1.4.3.3 | glycine + 2 H2O + O2 | poor substrate | Trigonopsis variabilis | 2 formic acid + NH3 + H2O2 | - |
? | |
| 1.4.3.3 | additional information | nearly inactive toward D-aspartic and D-glutamic acids | Trigonopsis variabilis | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.4.3.3 | D-amino acid oxidase | - |
Trigonopsis variabilis |
| 1.4.3.3 | DAAO | - |
Trigonopsis variabilis |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.4.3.3 | FAD | dependent | Trigonopsis variabilis | |
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Release 2023.1 (January 2023)
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