EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.2 | 0.0211 | - |
thiamine | - |
Paenibacillus thiaminolyticus | |
2.5.1.2 | 0.0211 | - |
thiamine | pH 7.2, temperature not specified in the publication | Paenibacillus thiaminolyticus | |
2.5.1.2 | 0.0364 | - |
4-nitrothiophenolate | - |
Paenibacillus thiaminolyticus | |
2.5.1.2 | 0.0364 | - |
4-nitrothiophenolate | pH 7.2, temperature not specified in the publication | Paenibacillus thiaminolyticus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.2 | Paenibacillus thiaminolyticus | - |
- |
- |
2.5.1.2 | Paenibacillus thiaminolyticus | P45741 | recombinant | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.5.1.2 | - |
Paenibacillus thiaminolyticus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.5.1.2 | additional information | - |
a method for measuring thiaminase I activity in complex samples is described. This assay is based on the selective consumption of the highly chromophoric 4-nitrothiophenolate by thiaminase I, resulting in a large decrease in absorbance at 411 nm. This assay is simple and sensitive, and it requires only readily available chemicals and a visible region spectrophotometer. The assay is optimized for high-throughput analysis in a 96-well format with complex biological samples | Paenibacillus thiaminolyticus |
2.5.1.2 | additional information | - |
method for measuring thiaminase activity in complex samples. The assay is based on the selective consumption of the highly chromophoric 4-nitrothiophenolate by thiaminase I, which is able to use a variety of nucleophiles as cosubstrates. This assay is sensitive and uses readily available chemicals and a visible region spectrophotometer. In addition, the assay can be easily performed in a high-throughput fashion in either 96- or 384-well plates | Paenibacillus thiaminolyticus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.2 | thiamine + 4-nitrothiophenolate | no activity with thiaminase II | Paenibacillus thiaminolyticus | 4-methyl-5-(2-hydroxyethyl)thiazole + 2-methyl-5[[(4-nitrophenyl)sulfanyl]methyl]pyrimidin-4-amine | - |
? | |
2.5.1.2 | thiamine + 4-nitrothiophenolate | - |
Paenibacillus thiaminolyticus | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.5.1.2 | thiaminase I | - |
Paenibacillus thiaminolyticus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.2 | 260 | - |
thiamine | - |
Paenibacillus thiaminolyticus | |
2.5.1.2 | 260 | - |
thiamine | pH 7.2, temperature not specified in the publication | Paenibacillus thiaminolyticus | |
2.5.1.2 | 297 | - |
4-nitrothiophenolate | - |
Paenibacillus thiaminolyticus | |
2.5.1.2 | 297 | - |
4-nitrothiophenolate | pH 7.2, temperature not specified in the publication | Paenibacillus thiaminolyticus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.5.1.2 | 7.2 | - |
assay at | Paenibacillus thiaminolyticus |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.2 | 8380 | - |
4-nitrothiophenolate | pH 7.2, temperature not specified in the publication | Paenibacillus thiaminolyticus | |
2.5.1.2 | 12400 | - |
thiamine | pH 7.2, temperature not specified in the publication | Paenibacillus thiaminolyticus |