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Literature summary extracted from

  • Pufahl, R.A.; Kasten, T.P.; Hills, R.; Gierse, J.K.; Reitz, B.A.; Weinberg, R.A.; Masferrer, J.L.
    Development of a fluorescence-based enzyme assay of human 5-lipoxygenase (2007), Anal. Biochem., 364, 204-212.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.13.11.34 ATP
-
 Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.34 from adipose tissue, expression of the enzyme in Spodoptera frugiperda Sf9 cells using the baculovirus transfection system, subcloning and propagation of the virus in Escherichia coli strain DH10 Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.34 CJ-13610
-
 Homo sapiens
1.13.11.34 glutathione inhibition is increased in presence of glutathione peroxidase Homo sapiens
1.13.11.34 nordihydroguaiaretic acid
-
 Homo sapiens
1.13.11.34 SC-756
-
 Homo sapiens
1.13.11.34 ZD-2138
-
 Homo sapiens
1.13.11.34 zileuton
-
 Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.34 Ca2+
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.34 (6E,8Z,11Z,14Z)-(5S)-5-hydroperoxyeicosa-6,8,11,14-tetraenoate Homo sapiens
-
 leukotriene A4 + H2O is unstable and metabolized to leukotriene B4 ?
1.13.11.34 arachidonic acid + O2 Homo sapiens first committed step in the synthesis of leukotrienes (6E,8Z,11Z,14Z)-(5S)-5-hydroperoxyeicosa-6,8,11,14-tetraenoate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.34 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.13.11.34 adipose tissue
-
 Homo sapiens
-
1.13.11.34 brain
-
 Homo sapiens
-
1.13.11.34 cerebellum
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.34 (6E,8Z,11Z,14Z)-(5S)-5-hydroperoxyeicosa-6,8,11,14-tetraenoate
-
 Homo sapiens leukotriene A4 + H2O
-
 ?
1.13.11.34 (6E,8Z,11Z,14Z)-(5S)-5-hydroperoxyeicosa-6,8,11,14-tetraenoate
-
 Homo sapiens leukotriene A4 + H2O is unstable and metabolized to leukotriene B4 ?
1.13.11.34 2',7'-dichlorodihydrofluorescein diacetate + O2 development of a fluorescence assay for 5-LOX, overview Homo sapiens ?
-
 ?
1.13.11.34 arachidonic acid + O2
-
 Homo sapiens (6E,8Z,11Z,14Z)-(5S)-5-hydroperoxyeicosa-6,8,11,14-tetraenoate
-
 ?
1.13.11.34 arachidonic acid + O2 first committed step in the synthesis of leukotrienes Homo sapiens (6E,8Z,11Z,14Z)-(5S)-5-hydroperoxyeicosa-6,8,11,14-tetraenoate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.11.34 5-lipoxygenase
-
 Homo sapiens
1.13.11.34 5-LOX
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.34 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.34 7.5
-
 assay at Homo sapiens

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.13.11.34 additional information
-
 enzyme inhibition in presence of inhibitor and glutathione peroxidase, overview Homo sapiens additional information
1.13.11.34 0.00009
-
 pH 7.5, 37°C, assay method via fluorescence measurement Homo sapiens nordihydroguaiaretic acid
1.13.11.34 0.00011 0.00012 pH 7.5, 37°C, dependent on assay method via fluorescence or UV measurement Homo sapiens ZD-2138
1.13.11.34 0.00012 0.00092 pH 7.5, 37°C, dependent on assay method via fluorescence or UV measurement Homo sapiens zileuton
1.13.11.34 0.00013
-
 pH 7.5, 37°C, assay method via fluorescence measurement Homo sapiens SC-756
1.13.11.34 0.00115 0.00155 pH 7.5, 37°C, dependent on assay method via fluorescence or UV measurement, kinetics, overview Homo sapiens CJ-13610