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Literature summary extracted from

  • Clarke, J.H.; Emson, P.C.; Irvine, R.F.
    Localization of Phosphatidylinositol phosphate Kinase II gamma in kidney to a membrane trafficking compartment within specialized cells of the nephron (2008), Am. J. Physiol. Renal Physiol., 295, F1422-1430.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.149 PIP5K2 genes are amplified from a whole human brain marathon-ready cDNA library using gene-specific primers. Recombinant protein is expressed from Escherichia coli harbouring PIP5K2s in pET-32a. PCR products are cloned, via incorporated HindIII and BamHI restriction sites, into appropriate plasmid vectors. HeLa, HEK 293, COS-7 and NRK cells are transiently transfected with plasmid constructs. Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.149 Golgi apparatus partial Golgi localization, primarily the matrix of the cis-Golgi Mus musculus 5794
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.149 47000
-
 predicted Homo sapiens
2.7.1.149 47000
-
 predicted and confirmed by western blotting, represents the phosphorylated form of mature PIP4Kgamma Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.149 ATP + 1-phosphatidyl-1D-myo-inositol 5-phosphate Mus musculus
-
 ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
 ir
2.7.1.149 ATP + 1-phosphatidyl-1D-myo-inositol 5-phosphate Homo sapiens
-
 ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.149 Homo sapiens Q8TBX8
-
-
2.7.1.149 Mus musculus Q91XU3
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.1.149 phosphoprotein
-
 Mus musculus

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.149 By using TALON metal affinity resin. Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.149 brain high mRNA expression Mus musculus
-
2.7.1.149 epithelial cell restricted expression in the thick ascending limb of the loop of Henle and in the intercalated cells of the collecting duct Mus musculus
-
2.7.1.149 heart high mRNA expression Mus musculus
-
2.7.1.149 heart PIP4Kgamma in heart run predominantly as a 40 kDa band, suggesting that processing of this isoform occurs. Mus musculus
-
2.7.1.149 HEK-293 cell
-
 Homo sapiens
-
2.7.1.149 HeLa cell
-
 Homo sapiens
-
2.7.1.149 kidney cortex and outer medulla, regulation of vesicular transport in specialized kidney epithelial cells Mus musculus
-
2.7.1.149 ovary
-
 Mus musculus
-
2.7.1.149 testis high mRNA expression Mus musculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.149 additional information
-
 Bacterially-expressed recombinant PIP4Kgamma is completely inactive, but does have the ability to associate with active PIP4Kalpha in vitro. Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.149 ATP + 1-phosphatidyl-1D-myo-inositol 5-phosphate
-
 Mus musculus ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
 ir
2.7.1.149 ATP + 1-phosphatidyl-1D-myo-inositol 5-phosphate
-
 Homo sapiens ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
 ir

Synonyms

EC Number Synonyms Comment Organism
2.7.1.149 1-phosphatidylinositol-5-phosphate 4-kinase
-
 Mus musculus
2.7.1.149 1-phosphatidylinositol-5-phosphate 4-kinase
-
 Homo sapiens
2.7.1.149 phosphatidylinositol phosphate kinase II gamma
-
 Mus musculus
2.7.1.149 phosphatidylinositol phosphate kinase II gamma
-
 Homo sapiens
2.7.1.149 PIP4K
-
 Mus musculus
2.7.1.149 PIP4K
-
 Homo sapiens
2.7.1.149 PIP4Kgamma isoform Mus musculus
2.7.1.149 PIP4Kgamma isoform Homo sapiens
2.7.1.149 PtdIns5P 4-kinase
-
 Mus musculus
2.7.1.149 PtdIns5P 4-kinase
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.149 ATP
-
 Mus musculus