EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.1.149 | PIP5K2 genes are amplified from a whole human brain marathon-ready cDNA library using gene-specific primers. Recombinant protein is expressed from Escherichia coli harbouring PIP5K2s in pET-32a. PCR products are cloned, via incorporated HindIII and BamHI restriction sites, into appropriate plasmid vectors. HeLa, HEK 293, COS-7 and NRK cells are transiently transfected with plasmid constructs. | Homo sapiens |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.7.1.149 | Golgi apparatus | partial Golgi localization, primarily the matrix of the cis-Golgi | Mus musculus | 5794 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.1.149 | 47000 | - |
predicted | Homo sapiens |
2.7.1.149 | 47000 | - |
predicted and confirmed by western blotting, represents the phosphorylated form of mature PIP4Kgamma | Mus musculus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.149 | ATP + 1-phosphatidyl-1D-myo-inositol 5-phosphate | Mus musculus | - |
ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | - |
ir | |
2.7.1.149 | ATP + 1-phosphatidyl-1D-myo-inositol 5-phosphate | Homo sapiens | - |
ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | - |
ir |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.1.149 | Homo sapiens | Q8TBX8 | - |
- |
2.7.1.149 | Mus musculus | Q91XU3 | - |
- |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
2.7.1.149 | phosphoprotein | - |
Mus musculus |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.1.149 | By using TALON metal affinity resin. | Homo sapiens |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.7.1.149 | brain | high mRNA expression | Mus musculus | - |
2.7.1.149 | epithelial cell | restricted expression in the thick ascending limb of the loop of Henle and in the intercalated cells of the collecting duct | Mus musculus | - |
2.7.1.149 | heart | high mRNA expression | Mus musculus | - |
2.7.1.149 | heart | PIP4Kgamma in heart run predominantly as a 40 kDa band, suggesting that processing of this isoform occurs. | Mus musculus | - |
2.7.1.149 | HEK-293 cell | - |
Homo sapiens | - |
2.7.1.149 | HeLa cell | - |
Homo sapiens | - |
2.7.1.149 | kidney | cortex and outer medulla, regulation of vesicular transport in specialized kidney epithelial cells | Mus musculus | - |
2.7.1.149 | ovary | - |
Mus musculus | - |
2.7.1.149 | testis | high mRNA expression | Mus musculus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.7.1.149 | additional information | - |
Bacterially-expressed recombinant PIP4Kgamma is completely inactive, but does have the ability to associate with active PIP4Kalpha in vitro. | Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.149 | ATP + 1-phosphatidyl-1D-myo-inositol 5-phosphate | - |
Mus musculus | ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | - |
ir | |
2.7.1.149 | ATP + 1-phosphatidyl-1D-myo-inositol 5-phosphate | - |
Homo sapiens | ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | - |
ir |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.1.149 | 1-phosphatidylinositol-5-phosphate 4-kinase | - |
Mus musculus |
2.7.1.149 | 1-phosphatidylinositol-5-phosphate 4-kinase | - |
Homo sapiens |
2.7.1.149 | phosphatidylinositol phosphate kinase II gamma | - |
Mus musculus |
2.7.1.149 | phosphatidylinositol phosphate kinase II gamma | - |
Homo sapiens |
2.7.1.149 | PIP4K | - |
Mus musculus |
2.7.1.149 | PIP4K | - |
Homo sapiens |
2.7.1.149 | PIP4Kgamma | isoform | Mus musculus |
2.7.1.149 | PIP4Kgamma | isoform | Homo sapiens |
2.7.1.149 | PtdIns5P 4-kinase | - |
Mus musculus |
2.7.1.149 | PtdIns5P 4-kinase | - |
Homo sapiens |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.149 | ATP | - |
Mus musculus |