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Literature summary extracted from

  • Karmohapatra, S.K.; Chakraborty, K.; Kahn, N.N.; Sinha, A.K.
    The role of nitric oxide in aspirin induced thrombolysis in vitro and the purification of aspirin activated nitric oxide synthase from human blood platelets (2007), Am. J. Hematol., 82, 986-995.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.13.39 Acetylsalicylic acid maximal activation at 0.004 mM Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.13.39 Ca2+ activates Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.13.39 19000
-
 gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.39 2 L-arginine + 3 NADPH + 3 H+ + 4 O2 Homo sapiens NO from acetylsalicylic acid-activated enzyme is involved in thrombolysis, overview 2 citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.39 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.39 native enzyme from platelets activated by acetylsalicylic acid, by solubilization with Triton X-100, anion exchange chromatography, and gel filtration, to homogeneity Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.13.39 blood platelet
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.39 2 L-arginine + 3 NADPH + 3 H+ + 4 O2
-
 Homo sapiens 2 citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O
-
 ?
1.14.13.39 2 L-arginine + 3 NADPH + 3 H+ + 4 O2 NO from acetylsalicylic acid-activated enzyme is involved in thrombolysis, overview Homo sapiens 2 citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.13.39 monomer 1 * 19000, SDS-PAGE Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.13.39 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.13.39 7.4
-
 assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.39 NADPH
-
 Homo sapiens