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Literature summary extracted from

  • Hou, C.X.; Fu, Z.Q.; Jin, B.R.; Gui, Z.Z.
    Purification and biochemical characterization of a novel glutathione S-transferase of the silkworm, Bombyx mori (2008), Afr. J. Biotechnol., 7, 311-316.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.18 albendazol 17.87% inhibition at 1 mM Bombyx mori
2.5.1.18 bile acid 41.78% inhibition at 1 mM Bombyx mori
2.5.1.18 NaCl 26.22% inhibition at 1 mM Bombyx mori
2.5.1.18 praziquantel 31.31% inhibition at 1 mM Bombyx mori

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.18 0.49
-
glutathione at 25°C Bombyx mori
2.5.1.18 0.494
-
1-chloro-2,4-dinitrobenzene at 25°C Bombyx mori

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.18 28000
-
SDS-PAGE Bombyx mori

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.18 Bombyx mori
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.18 glutathione agarose column chromatography Bombyx mori

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.18 0.728
-
crude extract, at 25°C Bombyx mori
2.5.1.18 4610
-
after 6332fold purification, at 25°C Bombyx mori

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.18 glutathione + 1-chloro-2,4-dinitrobenzene
-
Bombyx mori S-(2,4-dinitrophenyl)-glutathione + HCl
-
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Synonyms

EC Number Synonyms Comment Organism
2.5.1.18 glutathione S-transferase
-
Bombyx mori
2.5.1.18 GST
-
Bombyx mori

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.18 25
-
-
Bombyx mori

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.5.1.18 15 70 at temperatures ranging from 15°C to 70°C, the enzymatic activity increases with increasing temperature up to 25°C, then the activity starts to decrease Bombyx mori

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.18 57.87
-
glutathione at 25°C Bombyx mori
2.5.1.18 65.43
-
1-chloro-2,4-dinitrobenzene at 25°C Bombyx mori

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.18 7.1
-
-
Bombyx mori

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.5.1.18 5.1 9 at pH values ranging from 5.4-7.1, the enzymatic activity increases with increasing pH, then the activity starts to decrease Bombyx mori