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Literature summary extracted from

  • Xing, L.; Deng, X.; Kotedia, K.; Ackerstaff, E.; Ponomarev, V.; Clifton Ling, C.; Koutcher, J.A.; Li, G.C.
    Non-invasive molecular and functional imaging of cytosine deaminase and uracil phosphoribosyltransferase fused with red fluorescence protein (2008), Acta Oncol., 47, 1211-1220.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.4.2.9 medicine increased expression of UPRT may improve the antitumoral effect of 5-fluorouracil and 5-fluorocytosine, and thereby enhance the potential gene-directed enzyme prodrug therapy Homo sapiens
3.5.4.1 pharmacology applicability of gene-directed enzyme prodrug therapy, feasibility of using magnetic resonance spectroscopy and optical imaging to measure non-invasively expression and function of cytosine deaminase in a preclinical tumor model Saccharomyces cerevisiae

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.9 cytosine deaminase-UPRT-red fluorescence protein fusion proteins are expressed in Rattus norvegicus R-3327-AT cells Homo sapiens
3.5.4.1 expression vector with cytosine deaminase fused to monomeric DsRed (mDsRed), expression vector contains immediate early promoter of cytomegalovirus (CMV) and a hygromycin B resistance gene, transient transfection of COS-7 cells Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.4.1 cytosine + H2O Saccharomyces cerevisiae
-
 uracil + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.9 Homo sapiens Q96BW1
-
-
3.5.4.1 Saccharomyces cerevisiae Q12178
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.4.1 additional information
-
 development of cell lines and animal xenograft models, expression and function of fusion constructs between cytosine deaminase and monomeric DsRed (mDsRed), analyzed by Western blot, flow cytometry, fluorescence microscopy, magnetic resonance spectroscopy, analysis of antitumoral effect of 5-fluorouracil (5-FU) and 5-fluorocytosine (5-FC) in transgenic background, increase of cytosine deaminase enhances the potential of gene-directed enzyme prodrug therapy, conversion of 5-fluorocytosine to 5-fluorouracil analyzed in mice bearing tumor xenografts with the fusion product between cytosine deaminase and monomeric DsRed (R3327-CD/mDsRed) Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.9 uracil + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Homo sapiens UMP + diphosphate
-
 ?
3.5.4.1 5-fluorocytosine + H2O recombinant enzyme in gene therapy, expression analysis in transgenic mice with xenograft tumors, optical imaging technologies Saccharomyces cerevisiae 5-fluorouracil + NH3
-
 ?
3.5.4.1 cytosine + H2O
-
 Saccharomyces cerevisiae uracil + NH3
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.2.9 UPRT
-
 Homo sapiens
3.5.4.1 CD
-
 Saccharomyces cerevisiae