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Literature summary extracted from

  • Hu, G.; Li, L.; Li, D.; Liu, C.; Wei, S.; Liang, Y.; Su, X.
    Protein preparation and preliminary x-ray crystallographic analysis of a putative glucosamine 6-phosphate deaminase from Streptococcus mutans (2007), Acta Crystallogr. Sect. F, F63, 809-811.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.99.6 gene smu.636, cloning from genomic DNA, expression as His-tagged enzyme in Escherichia coli strain BL21(DE3) Streptococcus mutans

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.5.99.6 purified recombinant His-tagged enzyme, the protein solution contains 10 mg/ml protein in 20 mM Tris-HCl, 150 mM NaCl, pH 7.5, mixing of 0.001 ml of protein and 0.001 ml of reservoir solution, and equilibration against 0.5 ml of reservoir solution, hanging drop vapour diffusion method, X-ray diffraction structure determination and anaylsis at 2.4 A resolution Streptococcus mutans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.99.6 25500
-
 x * 25500, recombinant His-tagged enzyme, SDS-PAGE Streptococcus mutans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.99.6 glucosamine 6-phosphate + H2O Streptococcus mutans
-
 fructose 6-phosphate + NH3
-
 ?
3.5.99.6 glucosamine 6-phosphate + H2O Streptococcus mutans UA159
-
 fructose 6-phosphate + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.99.6 Streptococcus mutans
-
 gene smu.636
-
3.5.99.6 Streptococcus mutans UA159
-
 gene smu.636
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.99.6 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) to homogeneity in two steps by nickel affinity chromatography and gel filtration Streptococcus mutans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.99.6 glucosamine 6-phosphate + H2O
-
 Streptococcus mutans fructose 6-phosphate + NH3
-
 ?
3.5.99.6 glucosamine 6-phosphate + H2O
-
 Streptococcus mutans UA159 fructose 6-phosphate + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.99.6 ? x * 25500, recombinant His-tagged enzyme, SDS-PAGE Streptococcus mutans

Synonyms

EC Number Synonyms Comment Organism
3.5.99.6 NagB
-
 Streptococcus mutans
3.5.99.6 SMU.636 protein
-
 Streptococcus mutans