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Literature summary extracted from

  • Dreyfus, C.; Pignol, D.; Arnoux, P.
    Expression, purification, crystallization and preliminary X-ray analysis of an archaeal protein homologous to plant nicotianamine synthase (2008), Acta Crystallogr. Sect. F, 64, 933-935.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.43 expression in Escherichia coli Methanothermobacter thermautotrophicus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.5.1.43 diffraction to 1.7 A resolution, space group P212121 Methanothermobacter thermautotrophicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.43 30000
-
 and 60000, gel filtration Methanothermobacter thermautotrophicus
2.5.1.43 60000
-
 and 30000, gel filtration Methanothermobacter thermautotrophicus

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.43 Methanothermobacter thermautotrophicus
-
-
-
2.5.1.43 Methanothermobacter thermautotrophicus MTH675
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.5.1.43 additional information enzyme dimers are linked by at least one disulfide bridge Methanothermobacter thermautotrophicus

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.43 recombinant, His-tagged protein Methanothermobacter thermautotrophicus

Subunits

EC Number Subunits Comment Organism
2.5.1.43 dimer and monomer, 2 * 30000, SDS-PAGE, 2 * 3304, calculated, His-tagged protein Methanothermobacter thermautotrophicus
2.5.1.43 monomer and dimer, 1 * 30000, SDS-PAGE, 1 * 3304, calculated, His-tagged protein Methanothermobacter thermautotrophicus