EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.1.1.209 | purified recombinant enzyme, in a AKR1C21-NADPH binary complex, hanging drop vapour diffusion method, 22°C, 18 mg/ml of protein in 0.1 M HEPES, pH 7.5, 10% PEG 6000, and 5% 2-methyl-2,4-pentanediol, mixing with an equal volume of the crystallization buffer, and equilibration against 1 ml reservoir solution, X-ray diffraction structure determination and analysis at 1.8 A resolution | Mus musculus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.209 | 21-hydroxy-5beta-pregnane-3,20-dione + NADPH | Mus musculus | - |
21-hydroxy-5beta-pregnane-20alpha-ol-3-one + NADP+ | - |
r | |
1.1.1.209 | 5beta-pregnane-3alpha,20alpha-diol + NADPH + H+ | Mus musculus | - |
5beta-pregnane-20alpha-ol-3-one + NADP+ | - |
r | |
1.1.1.209 | androstenedione + NADPH + H+ | Mus musculus | - |
androsterone + NADP+ | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.209 | Mus musculus | Q91WR5 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.209 | 21-hydroxy-5beta-pregnane-3,20-dione + NADPH | - |
Mus musculus | 21-hydroxy-5beta-pregnane-20alpha-ol-3-one + NADP+ | - |
r | |
1.1.1.209 | 5beta-pregnane-3alpha,20alpha-diol + NADPH + H+ | - |
Mus musculus | 5beta-pregnane-20alpha-ol-3-one + NADP+ | - |
r | |
1.1.1.209 | androstenedione + NADPH + H+ | - |
Mus musculus | androsterone + NADP+ | - |
r | |
1.1.1.209 | additional information | a bifunctional enzyme that catalyses the oxidoreduction of the 3- and 17-hydroxy/keto groups of steroid substrates such as oestrogens, androgens and neurosteroids, 5beta-pregnane-3alpha,20alpha-diol, 21-hydroxy-5beta-pregnane-3,20-dione, and androstenedione are docked into the substrate-binding cavity and structure analysis, Lys31, Gly225 and Gly226 are important for ligand recognition and orientation in the active site, molecular-docking simulations, overview | Mus musculus | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.209 | 3(17)alpha-hydroxysteroid dehydrogenase | - |
Mus musculus |
1.1.1.209 | AKR1C21 | - |
Mus musculus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.209 | NADP+ | binding structure, overview | Mus musculus | |
1.1.1.209 | NADPH | binding structure, overview | Mus musculus |