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Literature summary extracted from

  • Kim, K.H.; Park, J.K.; Ha, B.H.; Moon, J.H.; Kim, E.E.
    Crystallization and preliminary X-ray crystallographic analysis of enoyl-ACP reductase III (FabL) from Bacillus subtilis (2007), Acta Crystallogr. Sect. F, 63, 246-248.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.10 expressed in Escherichia coli BL21(DE3) cells Bacillus subtilis
1.3.1.104 expression in Escherichia coli Bacillus subtilis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.3.1.10 hanging drop vapour diffusion method with 0.04 M MgCl2, 0.05 M sodium cacodylate pH 6.0 and 11% (v/v) 2-methyl-2,4-pentanediol at 4°C Bacillus subtilis
1.3.1.104 to 2.5 A resolution. Hexagonal space group P622, with unit-cell parameters a = b = 139.56, c = 62.75 A , alpha = beta = 90, gamma = 120° Bacillus subtilis

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.9 Bacillus subtilis
-
-
-
1.3.1.9 Bacillus subtilis 168
-
-
-
1.3.1.10 Bacillus subtilis
-
-
-
1.3.1.10 Bacillus subtilis 168
-
-
-
1.3.1.104 Bacillus subtilis P71079
-
-
1.3.1.104 Bacillus subtilis 168 P71079
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.10 nickel-chelated Hi-Trap column chromatography, Hi-Trap Blue HP column chromatography, Q-Sepharose column chromatography, and Superdex-75 gel filtration Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.9 acyl-[acyl-carrier protein] + NAD+
-
 Bacillus subtilis trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH + H+
-
 ?
1.3.1.9 acyl-[acyl-carrier protein] + NAD+
-
 Bacillus subtilis 168 trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH + H+
-
 ?
1.3.1.10 acyl-[acyl-carrier protein] + NADP+
-
 Bacillus subtilis trans-2,3-dehydroacyl-[acyl-carrier protein] + NADPH + H+
-
 ?
1.3.1.10 acyl-[acyl-carrier protein] + NADP+
-
 Bacillus subtilis 168 trans-2,3-dehydroacyl-[acyl-carrier protein] + NADPH + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.1.9 enoyl-ACP reductase III
-
 Bacillus subtilis
1.3.1.9 FabI
-
 Bacillus subtilis
1.3.1.9 NADH-ENR
-
 Bacillus subtilis
1.3.1.9 trans-enoyl-[acyl-carrier-protein] reductase
-
 Bacillus subtilis
1.3.1.10 enoyl-ACP reductase III
-
 Bacillus subtilis
1.3.1.10 FabL
-
 Bacillus subtilis
1.3.1.10 trans-enoyl-[acyl-carrier-protein] reductase
-
 Bacillus subtilis
1.3.1.104 FabL
-
 Bacillus subtilis

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.9 NAD+ dependent Bacillus subtilis
1.3.1.10 NADP+ dependent Bacillus subtilis