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Literature summary extracted from

  • Nakamura, Y.; Umehara, T.; Tanaka, A.; Horikoshi, M.; Padmanabhan, B.; Yokoyama, S.
    Purification, crystallization and preliminary X-ray diffraction analysis of the non-ATPase subunit Nas6 in complex with the ATPase subunit Rpt3 of the 26S proteasome from Saccharomyces cerevisiae (2007), Acta Crystallogr. Sect. F, 63, 190-192.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.6.1.5 the cDNA encoding Nas6 and the cDNA encoding the C-terminal region of Rpt3, residues 348-428, are subcloned into the first and second cassette of pETDuet1, respectively Saccharomyces cerevisiae

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.6.1.5 the structure of the complex between the non-ATPase subunit Nas6 and the C-terminal domain of the proteasomal ATPase Rpt3 is determined at a resolution of 2.2 A Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.6.1.5 6000
-
 C-terminal domain of Rpt3, Rpt3C, determined by SDS-PAGE Saccharomyces cerevisiae
5.6.1.5 35000
-
 Nas6-Rpt3C complex, determined by SDS-PAGE Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.6.1.5 ATP + H2O + polypeptide Saccharomyces cerevisiae
-
 ADP + phosphate + unfolded polypeptide
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
5.6.1.5 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.6.1.5 the Nas6-Rpt3C complex, expressed in Escherichia coli Rosetta DE3 cells, is purified on a HisTrap HP, a HiTrapQ anion-exchange, and a gel-filtration column Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.6.1.5 ATP + H2O + polypeptide
-
 Saccharomyces cerevisiae ADP + phosphate + unfolded polypeptide
-
 ir

Subunits

EC Number Subunits Comment Organism
5.6.1.5 heterohexamer the base structure of the 19S regulatory particle consists of heterohexameric proteasomal ATPases, Rpt1-Rpt6 in yeast Saccharomyces cerevisiae

Synonyms

EC Number Synonyms Comment Organism
5.6.1.5 26S proteasome
-
 Saccharomyces cerevisiae
5.6.1.5 ATPase subunit Rpt3
-
 Saccharomyces cerevisiae
5.6.1.5 proteasome ATPase
-
 Saccharomyces cerevisiae
5.6.1.5 Rpt3
-
 Saccharomyces cerevisiae