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Literature summary extracted from

  • Fourrat, L.; Iddar, A.; Soukri, A.
    Purification and characterization of cytosolic glyceraldehyde-3-phosphate dehydrogenase from the dromedary camel (2007), Acta Biochim. Biophys. Sin. (Shanghai), 39, 148-154.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.12 Cd2+ strong inhibition Camelus dromedarius
1.2.1.12 Cu2+
-
 Camelus dromedarius
1.2.1.12 Hg2+ strong inhibition Camelus dromedarius
1.2.1.12 K+
-
 Camelus dromedarius
1.2.1.12 Mn2+
-
 Camelus dromedarius
1.2.1.12 Zn2+
-
 Camelus dromedarius

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.12 0.025
-
 NAD+ in 50 mM tricine-NaOH buffer (pH 8.5) with 10 mM sodium arsenate, at 25°C Camelus dromedarius
1.2.1.12 0.21
-
 D-glyceraldehyde 3-phosphate in 50 mM tricine-NaOH buffer (pH 8.5) with 10 mM sodium arsenate, at 25°C Camelus dromedarius

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.2.1.12 cytosol
-
 Camelus dromedarius 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.12 36500
-
 4 * 36500, non-denaturing PAGE Camelus dromedarius
1.2.1.12 37000
-
 SDS-PAGE Camelus dromedarius
1.2.1.12 146000
-
 non-denaturing PAGE Camelus dromedarius

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.12 Camelus dromedarius
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.12 ammonium sulfate fractionation and Blue Sepharose column chromatography Camelus dromedarius

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.12 skeletal muscle
-
 Camelus dromedarius
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.12 1.68
-
 crude extract, at 25°C Camelus dromedarius
1.2.1.12 45.1
-
 after 26.8fold purification, at 25°C Camelus dromedarius

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.12 D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 Camelus dromedarius 3-phospho-D-glyceroyl phosphate + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.12 homotetramer 4 * 37000, SDS-PAGE Camelus dromedarius
1.2.1.12 homotetramer 4 * 36500, non-denaturing PAGE Camelus dromedarius

Synonyms

EC Number Synonyms Comment Organism
1.2.1.12 GAPDH
-
 Camelus dromedarius

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.1.12 28 32
-
 Camelus dromedarius

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.2.1.12 15 70 pre-incubation for 10 min at temperatures varying between 20 and 32°C does not irreversibly affect the enzyme activity, thermal inactivation occurs above 35°C and results in total activity loss at 70°C Camelus dromedarius

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.12 7.8
-
-
 Camelus dromedarius

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.2.1.12 7 8.5
-
 Camelus dromedarius

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.12 NAD+ dependent Camelus dromedarius

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.2.1.12 Camelus dromedarius isoelectric focusing
-
 7.2

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.2.1.12 4.6
-
 at 25°C Camelus dromedarius Cd2+
1.2.1.12 6.2
-
 at 25°C Camelus dromedarius Hg2+
1.2.1.12 14.2
-
 at 25°C Camelus dromedarius Zn2+
1.2.1.12 22.7
-
 at 25°C Camelus dromedarius Cu2+
1.2.1.12 25.8
-
 at 25°C Camelus dromedarius Mn2+
1.2.1.12 34.6
-
 at 25°C Camelus dromedarius K+