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Literature summary extracted from

  • Swenson, S.; Markland, F.S.
    Fibrolase (2006), Toxin Rev., 25, 351-378.
No PubMed abstract available

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.4.24.72 structure analysis Agkistrodon contortrix contortrix

Protein Variants

EC Number Protein Variants Comment Organism
3.4.24.72 additional information construction of a heterobifunctional chimeric derivative of fibrolase through linking covalently with a RGD-like peptide via a surface residue distant from the active site, structure, overview, the chimera shows fibrolase and platelet-binding ability, the chimeric enzyme exhibits an IC50 of 105 nM in inhibition of platelet aggregation, overview Agkistrodon contortrix contortrix

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.72 1,10-phenanthroline rapid and complete inhibition Agkistrodon contortrix contortrix
3.4.24.72 alpha2-Macroglobulin the 720 kDa tetrameric inhibitor causes rapid and irreversible inhibition by sterical hindrance, the PEG-bound enzyme is less sensitive Agkistrodon contortrix contortrix
3.4.24.72 EDTA rapid and complete inhibition Agkistrodon contortrix contortrix
3.4.24.72 additional information polyethylene glycol addition to the enzyme modifies the enzyme size and reduces its activity Agkistrodon contortrix contortrix
3.4.24.72 tetraethylene pentamine rapid and complete inhibition Agkistrodon contortrix contortrix

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.24.72 extracellular
-
 Agkistrodon contortrix contortrix
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.72 Zn2+ 1 mol per mol of enzyme, fibrolase is a zinc metalloprotease with the zinc binding motif HEXXHXXGXXH Agkistrodon contortrix contortrix

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.72 23000
-
 x * 23000, native enzyme, x * 60000, PEG-bound enzyme Agkistrodon contortrix contortrix
3.4.24.72 43330
-
 PEG-bound enzyme, gel filtration Agkistrodon contortrix contortrix
3.4.24.72 60000
-
 x * 23000, native enzyme, x * 60000, PEG-bound enzyme Agkistrodon contortrix contortrix

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.24.72 Fibrin + H2O Agkistrodon contortrix contortrix fibrinolytic activity ?
-
 ?
3.4.24.72 fibrinogen + H2O Agkistrodon contortrix contortrix fibrinogenolytic activity fibrin + propeptide
-
 ?
3.4.24.72 low-molecular weight kininogen + H2O Agkistrodon contortrix contortrix initiates generation of bradykinin kallidin + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.72 Agkistrodon contortrix contortrix
-
 southern copperhead snake
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.72 native enzyme by hydrophobic interaction and hydroxyapatite chromatography Agkistrodon contortrix contortrix

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.24.72 venom
-
 Agkistrodon contortrix contortrix
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.24.72 1200
-
 purified PEG-bound enzyme, substrate azocasein Agkistrodon contortrix contortrix
3.4.24.72 1260
-
 purified native enzyme, substrate azocasein Agkistrodon contortrix contortrix
3.4.24.72 10900
-
 purified PEG-bound enzyme, substrate human fibrin Agkistrodon contortrix contortrix
3.4.24.72 11300
-
 purified native enzyme, substrate human fibrin Agkistrodon contortrix contortrix

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.72 4-amino-benzene-His-Thr-Glu-Lys-leu-Val-Thr-Ser-dinitrophenol + H2O
-
 Agkistrodon contortrix contortrix ?
-
 ?
3.4.24.72 azocasein + H2O
-
 Agkistrodon contortrix contortrix ?
-
 ?
3.4.24.72 Fibrin + H2O fibrinolytic activity Agkistrodon contortrix contortrix ?
-
 ?
3.4.24.72 Fibrin + H2O human substrate, fibrinolytic activity cleaving the Lys413-Leu414 bond Agkistrodon contortrix contortrix ?
-
 ?
3.4.24.72 fibrinogen + H2O fibrinogenolytic activity Agkistrodon contortrix contortrix fibrin + propeptide
-
 ?
3.4.24.72 His-Thr-Glu-Ala-Leu-Val-Thr-Ser + H2O
-
 Agkistrodon contortrix contortrix ?
-
 ?
3.4.24.72 His-Thr-Glu-Arg-Leu-Val-Thr-Ser + H2O
-
 Agkistrodon contortrix contortrix ?
-
 ?
3.4.24.72 His-Thr-Glu-Asn-Leu-Val-Thr-Ser + H2O
-
 Agkistrodon contortrix contortrix ?
-
 ?
3.4.24.72 His-Thr-Glu-Glu-Leu-Val-Thr-Ser + H2O
-
 Agkistrodon contortrix contortrix ?
-
 ?
3.4.24.72 His-Thr-Glu-His-Leu-Val-Thr-Ser + H2O
-
 Agkistrodon contortrix contortrix ?
-
 ?
3.4.24.72 His-Thr-Glu-Leu-Leu-Val-Thr-Ser + H2O
-
 Agkistrodon contortrix contortrix ?
-
 ?
3.4.24.72 His-Thr-Glu-Lys-Leu-Val-Thr-Ser + H2O cleaving sequence residues 410-417 from fibrinogen Aalpha-chain Agkistrodon contortrix contortrix ?
-
 ?
3.4.24.72 His-Thr-Glu-Phe-Leu-Val-Thr-Ser + H2O
-
 Agkistrodon contortrix contortrix ?
-
 ?
3.4.24.72 His-Thr-Glu-Pro-Leu-Val-Thr-Ser + H2O
-
 Agkistrodon contortrix contortrix ?
-
 ?
3.4.24.72 His-Thr-Glu-Ser-Leu-Val-Thr-Ser + H2O
-
 Agkistrodon contortrix contortrix ?
-
 ?
3.4.24.72 Insulin B-chain + H2O fibrinogenolytic activity cleaving the Leu11-Val-Glu-Ala-Leu-Tyr-Leu-Val18 peptide Agkistrodon contortrix contortrix ?
-
 ?
3.4.24.72 Leu-Val-Glu-Ala-Leu-Tyr-Leu-Val + H2O cleaving sequence residues 11-18 from insulin B-chain Agkistrodon contortrix contortrix ?
-
 ?
3.4.24.72 low-molecular weight kininogen + H2O
-
 Agkistrodon contortrix contortrix kallidin + ?
-
 ?
3.4.24.72 low-molecular weight kininogen + H2O initiates generation of bradykinin Agkistrodon contortrix contortrix kallidin + ?
-
 ?
3.4.24.72 additional information the enzyme is a nonhemorrhagic zinc metalloprotease, peptide substrate specificity, preference for Xaa-Leu and Xaa-Phe overview Agkistrodon contortrix contortrix ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.24.72 ? x * 23000, native enzyme, x * 60000, PEG-bound enzyme Agkistrodon contortrix contortrix
3.4.24.72 More the protein is composed of 5 alpha-helical regions, 4 parallel and 1 antiparallel beta-sheet, stablized by 3 disulfide bonds Agkistrodon contortrix contortrix

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.72 7.4
-
-
 Agkistrodon contortrix contortrix

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.24.72 additional information
-
 additional information native and PEG-bound enzyme, rapid interaction kinetics with inhibitor alpha2-macroglobulin Agkistrodon contortrix contortrix

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.4.24.72 Agkistrodon contortrix contortrix
-
-
 6.8