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Literature summary extracted from

  • Dhanda, S.; Singh, H.; Singh, J.; Singh, T.P.
    Isolation, purification and characterization of a DPP-III homologue from goat brain (2007), Protein Expr. Purif., 52, 297-305.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.14.4 1-ethyl-3(3-dimethyl-amino-propyl)-carbodiimide*HCl 1.0 mM inhibitor, 29% inhibition, 5.0 mM inhibitor, 64% inhibition Capra hircus
3.4.14.4 2,2'-dithiodipyridine 1.0 mM inhibitor, 32% inhibition, 2.5 mM inhibitor, 98% inhibition Capra hircus
3.4.14.4 2,4,6-trinitrobenzene sufonic acid trihydrate 1.0 mM inhibitor, 52% inhibition, 2.5 mM inhibitor, 89% inhibition Capra hircus
3.4.14.4 alpha1 anti-trypsin 1.0 mM inhibitor, 61% inhibition Capra hircus
3.4.14.4 Aprotinin 1.0 mM inhibitor, 59% inhibition, 5.0 mM inhibitor, 99% inhibition Capra hircus
3.4.14.4 benzamidine HCl 1.0 mM inhibitor, 29% inhibition, 5.0 mM inhibitor, 61% inhibition Capra hircus
3.4.14.4 DTNB 1.0 mM inhibitor, 54% inhibition, 2.5 mM inhibitor, 87% inhibition Capra hircus
3.4.14.4 EDTA 1.0 mM inhibitor, 50% inhibition, 5.0 mM inhibitor, 64% inhibition Capra hircus
3.4.14.4 Egg white trypsin inhibitor 1.0 mM inhibitor, 34% inhibition Capra hircus
3.4.14.4 iodoacetamide 1.0 mM inhibitor, 27% inhibition, 5.0 mM inhibitor, 42% inhibition Capra hircus
3.4.14.4 iodoacetic acid 1.0 mM inhibitor, 25% inhibition, 5.0 mM inhibitor, 48% inhibition Capra hircus
3.4.14.4 leupeptin 1.0 mM inhibitor, 32% inhibition Capra hircus
3.4.14.4 N-ethylmaleimide 1.0 mM inhibitor, 29% inhibition, 2.5 mM inhibitor, 88% inhibition, 5.0 mM inhibitor, 96% inhibition Capra hircus
3.4.14.4 N-hydroxy-succinimide 1.0 mM inhibitor, 30% inhibition, 5.0 mM inhibitor, 51% inhibition Capra hircus
3.4.14.4 o-phenanthroline 1.0 mM inhibitor, 46% inhibition, 2.5 mM inhibitor, 85% inhibition, 5.0 mM inhibitor, 96% inhibition Capra hircus
3.4.14.4 p-aminophenyl mercuric acetate 1.0 mM inhibitor, 42% inhibition, 2.5 mM inhibitor, 94% inhibition Capra hircus
3.4.14.4 p-hydroxymercuriphenyl-sulfonic acid 1.0 mM inhibitor, 22% inhibition, 2.5 mM inhibitor, 96% inhibition Capra hircus
3.4.14.4 p-nitrocatecholsulfate 1.0 mM inhibitor, 37% inhibition, 5.0 mM inhibitor, 58% inhibition Capra hircus
3.4.14.4 Pb(CH3COO)2 1.0 mM inhibitor, 53% inhibition, 2.5 mM inhibitor, 97% inhibition Capra hircus
3.4.14.4 pepstatin A 5.0 mM inhibitor, 92% inhibition Capra hircus
3.4.14.4 PMSF 1.0 mM inhibitor, 53% inhibition, 2.5 mM inhibitor, 87% inhibition Capra hircus
3.4.14.4 puromycin 1.0 mM inhibitor, 30% inhibition, 5.0 mM inhibitor, 58% inhibition Capra hircus
3.4.14.4 Soybean trypsin inhibitor 1.0 mM inhibitor, 64% inhibition, 2.5 mM inhibitor, 76% inhibition Capra hircus
3.4.14.4 TLCK 1.0 mM inhibitor, 27% inhibition, 5.0 mM inhibitor, 63% inhibition Capra hircus
3.4.14.4 TPCK 1.0 mM inhibitor, 75% inhibition, 5.0 mM inhibitor, 88% inhibition Capra hircus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.14.4 0.039
-
Arg-Arg-4-methoxy-beta-naphthylamide
-
Capra hircus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.14.4 69000
-
determined by SDS-PAGE, gel filtration and MALDI-TOF analysis Capra hircus

Organism

EC Number Organism UniProt Comment Textmining
3.4.14.4 Capra hircus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.14.4 DPP from goat brain is purified by several steps, including acid precipitation, ammonium sulfate fractionation, chromatography on a Sephadex G-100 column, cation, anion and adsorption chromatography Capra hircus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.14.4 brain
-
Capra hircus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.14.4 0.0001
-
purification step, homogenate Capra hircus
3.4.14.4 0.0004
-
purification step, acid precipitation Capra hircus
3.4.14.4 0.0012
-
purification step, ammonium sulfate fractionation, 35-65% pellet Capra hircus
3.4.14.4 0.0092
-
purification step, gel filtration chromatography on a Sephadex G-100 column Capra hircus
3.4.14.4 0.0126
-
purification step, cation exchange chromatography on a CM Sephadex A-50 column Capra hircus
3.4.14.4 0.0652
-
purification step, anion exchange chromatography on a DEAE Sephadex A-50 column Capra hircus
3.4.14.4 0.107
-
purification step, adsorption chromatography on a hydroxyapatite column Capra hircus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.14.4 Ala-Ala-beta-naphthylamide + H2O 10.0% activity Capra hircus Ala-Ala + beta-naphthylamine
-
?
3.4.14.4 Arg-Arg-4-methoxy-beta-naphthylamide + H2O activity assay, 100% activity Capra hircus Arg-Arg + 4-methoxy-beta-naphthylamine
-
?
3.4.14.4 Asp-Ala-beta-naphthylamide + H2O 0% activity Capra hircus Asp-Ala + beta-naphthylamine
-
?
3.4.14.4 Asp-Arg-beta-naphthylamide + H2O 1.5% activity Capra hircus Asp-Arg + beta-naphthylamine
-
?
3.4.14.4 Gly-Ala-beta-naphthylamide + H2O 3.2% activity Capra hircus Gly-Ala + beta-naphthylamine
-
?
3.4.14.4 Gly-Arg-beta-naphthylamide + H2O 11.8% activity Capra hircus Gly-Arg + beta-naphthylamine
-
?
3.4.14.4 Gly-Phe-beta-naphthylamide + H2O 2.4% activity Capra hircus Gly-Phe + beta-naphthylamine
-
?
3.4.14.4 Gly-Pro-4-methoxy-beta-naphthylamide + H2O 0% activity Capra hircus Gly-Pro + 4-methoxy-beta-naphthylamine
-
?
3.4.14.4 His-Phe-beta-naphthylamide + H2O 0% activity Capra hircus His-Phe + beta-naphthylamine
-
?
3.4.14.4 His-Ser-beta-naphthylamide + H2O 0% activity Capra hircus His-Ser + beta-naphthylamine
-
?
3.4.14.4 Leu-Ala-beta-naphthylamide + H2O 5.0% activity Capra hircus Leu-Ala + beta-naphthylamine
-
?
3.4.14.4 Lys-Ala-4-methoxy-beta-naphthylamide + H2O 5.0% activity Capra hircus Lys-Ala + 4-methoxy-beta-naphthylamine
-
?
3.4.14.4 Phe-Arg-beta-naphthylamide + H2O 17.6% activity Capra hircus Phe-Arg + beta-naphthylamine
-
?
3.4.14.4 Ser-Met-beta-naphthylamide + H2O 0% activity Capra hircus Ser-Met + beta-naphthylamine
-
?
3.4.14.4 Ser-Tyr-beta-naphthylamide + H2O 0% activity Capra hircus Ser-Tyr + beta-naphthylamine
-
?

Subunits

EC Number Subunits Comment Organism
3.4.14.4 monomer
-
Capra hircus

Synonyms

EC Number Synonyms Comment Organism
3.4.14.4 dipeptidyl peptidase III
-
Capra hircus
3.4.14.4 DPP III
-
Capra hircus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.14.4 37
-
activity assay Capra hircus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.4.14.4 40
-
the enzyme shows a considerable heat stability up to 40°C, 30% activity is lost at 45°C Capra hircus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.14.4 0.039
-
Arg-Arg-4-methoxy-beta-naphthylamide
-
Capra hircus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.14.4 8.5
-
activity assay Capra hircus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.14.4 6 11 the enzyme has 50% activity at pH 6 in the lower range and at pH 11 in the higher range Capra hircus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.4.14.4 Capra hircus
-
-
4.5