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Literature summary extracted from

  • Bozzo, G.G.; Basset, G.J.; Naponelli, V.; Noiriel, A.; Gregory, J.F.; Hanson, A.D.
    Characterization of the folate salvage enzyme p-aminobenzoylglutamate hydrolase in plants (2008), Phytochemistry, 69, 29-37.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.B18 1,10-phenanthroline 25% inhibition at 0.1 mM Arabidopsis thaliana
3.5.1.B18 8-Hydroxyquinoline-5-sulfonate complete inhibition at 1 mM Arabidopsis thaliana
3.5.1.B18 Cu2+ complete inhibition Arabidopsis thaliana
3.5.1.B18 DTNB 25% inhibition at 1 mM Arabidopsis thaliana
3.5.1.B18 DTT 53% inhibition at 1 mM Arabidopsis thaliana
3.5.1.B18 EDTA 21% inhibition at 1 mM Arabidopsis thaliana
3.5.1.B18 folate substrate inhibition, competitive Arabidopsis thaliana
3.5.1.B18 Hg2+ complete inhibition Arabidopsis thaliana
3.5.1.B18 additional information the enzyme activity is fairly insensitive to serine, cysteine, and aspartic reagents, but is inhibited by metal chelators Arabidopsis thaliana
3.5.1.B18 N,N,N',N'-tetrakis[2-pyridylmethyl]ethylenediamine i.e. TPEN, 53% inhibition at 0.1 mM Arabidopsis thaliana
3.5.1.B18 pepstatin 25% inhibition at 0.0015 mM Arabidopsis thaliana
3.5.1.B18 PMSF 15% inhibition at 1 mM Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.B18 additional information
-
 additional information kinetics Arabidopsis thaliana
3.5.1.B18 0.37
-
 4-aminobenzoylglutamate pH 7.4, 37°C Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.1.B18 cytosol
-
 Pisum sativum 5829
-
3.5.1.B18 mitochondrion
-
 Pisum sativum 5739
-
3.5.1.B18 additional information no activity in chloroplasts, the enzyme is probably also partially located in vacuoles Pisum sativum
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.B18 Mn2+ 54% activation at 0.1 mM, metalloenzyme Arabidopsis thaliana
3.5.1.B18 additional information metalloenzyme Spinacia oleracea
3.5.1.B18 additional information metalloenzyme Pisum sativum
3.5.1.B18 additional information metalloenzyme Zea mays
3.5.1.B18 additional information metalloenzyme Solanum lycopersicum
3.5.1.B18 additional information no activation by Zn2+ and Ni2+ Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.B18 200000
-
 main peak, gel filtration Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.B18 4-aminobenzoylglutamate + H2O Spinacia oleracea the enzyme is involved in the folate salvage pathway, overview 4-aminobenzoic acid + L-glutamate
-
 ?
3.5.1.B18 4-aminobenzoylglutamate + H2O Pisum sativum the enzyme is involved in the folate salvage pathway, overview 4-aminobenzoic acid + L-glutamate
-
 ?
3.5.1.B18 4-aminobenzoylglutamate + H2O Zea mays the enzyme is involved in the folate salvage pathway, overview 4-aminobenzoic acid + L-glutamate
-
 ?
3.5.1.B18 4-aminobenzoylglutamate + H2O Arabidopsis thaliana the enzyme is involved in the folate salvage pathway, overview 4-aminobenzoic acid + L-glutamate
-
 ?
3.5.1.B18 4-aminobenzoylglutamate + H2O Solanum lycopersicum the enzyme is involved in the folate salvage pathway, overview 4-aminobenzoic acid + L-glutamate
-
 ?
3.5.1.B18 folic acid + H2O Spinacia oleracea
-
 pteroic acid + L-glutamate
-
 ?
3.5.1.B18 folic acid + H2O Pisum sativum
-
 pteroic acid + L-glutamate
-
 ?
3.5.1.B18 folic acid + H2O Zea mays
-
 pteroic acid + L-glutamate
-
 ?
3.5.1.B18 folic acid + H2O Arabidopsis thaliana
-
 pteroic acid + L-glutamate
-
 ?
3.5.1.B18 folic acid + H2O Solanum lycopersicum
-
 pteroic acid + L-glutamate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.B18 Arabidopsis thaliana
-
 ecotype Columbia
-
3.5.1.B18 Pisum sativum
-
 cv. Laxton’s Progress 9
-
3.5.1.B18 Solanum lycopersicum
-
 cv. Micro-Tom
-
3.5.1.B18 Spinacia oleracea
-
-
-
3.5.1.B18 Zea mays
-
 cv. NK508
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.B18 native enzyme from 268fold leaves and 86fold from roots by ammonium sulfate fractionation, hydrophobic interaction and anion exchange chromatography, followed by gel filtration, activity loss during purification exceeds 95% Arabidopsis thaliana

Reaction

EC Number Reaction Comment Organism Reaction ID
3.5.1.B18 N2-(4-aminobenzoyl)-L-glutamate + H2O = 4-aminobenzoate + L-glutamate distinct from carboxypeptidase G, EC 3.4.17.11, and glutamate carboxypeptidase II, EC 3.4.17.21 Arabidopsis thaliana
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.B18 fruit
-
 Solanum lycopersicum
-
3.5.1.B18 leaf
-
 Spinacia oleracea
-
3.5.1.B18 leaf
-
 Pisum sativum
-
3.5.1.B18 leaf
-
 Zea mays
-
3.5.1.B18 leaf
-
 Arabidopsis thaliana
-
3.5.1.B18 leaf
-
 Solanum lycopersicum
-
3.5.1.B18 mesophyll
-
 Pisum sativum
-
3.5.1.B18 root high enzyme level, several fold higher than in leaves Spinacia oleracea
-
3.5.1.B18 root high enzyme level, several fold higher than in leaves Pisum sativum
-
3.5.1.B18 root high enzyme level, several fold higher than in leaves Zea mays
-
3.5.1.B18 root high enzyme level, several fold higher than in leaves Solanum lycopersicum
-
3.5.1.B18 root in vitro tissue culture, high enzyme level, several fold higher than in leaves Arabidopsis thaliana
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.B18 additional information
-
 the enzyme shows very low activity and is a rare enzyme Spinacia oleracea
3.5.1.B18 additional information
-
 the enzyme shows very low activity and is a rare enzyme Pisum sativum
3.5.1.B18 additional information
-
 the enzyme shows very low activity and is a rare enzyme Zea mays
3.5.1.B18 additional information
-
 the enzyme shows very low activity and is a rare enzyme Arabidopsis thaliana
3.5.1.B18 additional information
-
 the enzyme shows very low activity and is a rare enzyme Solanum lycopersicum
3.5.1.B18 0.00029
-
 purified enzyme from roots Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.B18 4-aminobenzoylglutamate + H2O
-
 Spinacia oleracea 4-aminobenzoic acid + L-glutamate
-
 ?
3.5.1.B18 4-aminobenzoylglutamate + H2O
-
 Pisum sativum 4-aminobenzoic acid + L-glutamate
-
 ?
3.5.1.B18 4-aminobenzoylglutamate + H2O
-
 Zea mays 4-aminobenzoic acid + L-glutamate
-
 ?
3.5.1.B18 4-aminobenzoylglutamate + H2O
-
 Arabidopsis thaliana 4-aminobenzoic acid + L-glutamate
-
 ?
3.5.1.B18 4-aminobenzoylglutamate + H2O
-
 Solanum lycopersicum 4-aminobenzoic acid + L-glutamate
-
 ?
3.5.1.B18 4-aminobenzoylglutamate + H2O the enzyme is involved in the folate salvage pathway, overview Spinacia oleracea 4-aminobenzoic acid + L-glutamate
-
 ?
3.5.1.B18 4-aminobenzoylglutamate + H2O the enzyme is involved in the folate salvage pathway, overview Pisum sativum 4-aminobenzoic acid + L-glutamate
-
 ?
3.5.1.B18 4-aminobenzoylglutamate + H2O the enzyme is involved in the folate salvage pathway, overview Zea mays 4-aminobenzoic acid + L-glutamate
-
 ?
3.5.1.B18 4-aminobenzoylglutamate + H2O the enzyme is involved in the folate salvage pathway, overview Arabidopsis thaliana 4-aminobenzoic acid + L-glutamate
-
 ?
3.5.1.B18 4-aminobenzoylglutamate + H2O the enzyme is involved in the folate salvage pathway, overview Solanum lycopersicum 4-aminobenzoic acid + L-glutamate
-
 ?
3.5.1.B18 folic acid + H2O
-
 Spinacia oleracea pteroic acid + L-glutamate
-
 ?
3.5.1.B18 folic acid + H2O
-
 Pisum sativum pteroic acid + L-glutamate
-
 ?
3.5.1.B18 folic acid + H2O
-
 Zea mays pteroic acid + L-glutamate
-
 ?
3.5.1.B18 folic acid + H2O
-
 Arabidopsis thaliana pteroic acid + L-glutamate
-
 ?
3.5.1.B18 folic acid + H2O
-
 Solanum lycopersicum pteroic acid + L-glutamate
-
 ?
3.5.1.B18 additional information the enzyme is distinct from carboxypeptidase G, EC 3.4.17.11, which also shows p-aminobenzoylglutamate hydrolase activity, overview Spinacia oleracea ?
-
 ?
3.5.1.B18 additional information the enzyme is distinct from carboxypeptidase G, EC 3.4.17.11, which also shows p-aminobenzoylglutamate hydrolase activity, overview Pisum sativum ?
-
 ?
3.5.1.B18 additional information the enzyme is distinct from carboxypeptidase G, EC 3.4.17.11, which also shows p-aminobenzoylglutamate hydrolase activity, overview Zea mays ?
-
 ?
3.5.1.B18 additional information the enzyme is distinct from carboxypeptidase G, EC 3.4.17.11, which also shows p-aminobenzoylglutamate hydrolase activity, overview Arabidopsis thaliana ?
-
 ?
3.5.1.B18 additional information the enzyme is distinct from carboxypeptidase G, EC 3.4.17.11, which also shows p-aminobenzoylglutamate hydrolase activity, overview Solanum lycopersicum ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.B18 oligomer
-
 Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
3.5.1.B18 p-aminobenzoylglutamate hydrolase
-
 Spinacia oleracea
3.5.1.B18 p-aminobenzoylglutamate hydrolase
-
 Pisum sativum
3.5.1.B18 p-aminobenzoylglutamate hydrolase
-
 Zea mays
3.5.1.B18 p-aminobenzoylglutamate hydrolase
-
 Arabidopsis thaliana
3.5.1.B18 p-aminobenzoylglutamate hydrolase
-
 Solanum lycopersicum
3.5.1.B18 pABAGlu hydrolase
-
 Spinacia oleracea
3.5.1.B18 pABAGlu hydrolase
-
 Pisum sativum
3.5.1.B18 pABAGlu hydrolase
-
 Zea mays
3.5.1.B18 pABAGlu hydrolase
-
 Arabidopsis thaliana
3.5.1.B18 pABAGlu hydrolase
-
 Solanum lycopersicum
3.5.1.B18 PGH
-
 Spinacia oleracea
3.5.1.B18 PGH
-
 Pisum sativum
3.5.1.B18 PGH
-
 Zea mays
3.5.1.B18 PGH
-
 Arabidopsis thaliana
3.5.1.B18 PGH
-
 Solanum lycopersicum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.B18 37
-
 assay at Spinacia oleracea
3.5.1.B18 37
-
 assay at Pisum sativum
3.5.1.B18 37
-
 assay at Zea mays
3.5.1.B18 37
-
 assay at Arabidopsis thaliana
3.5.1.B18 37
-
 assay at Solanum lycopersicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.B18 7.3 7.5
-
 Arabidopsis thaliana
3.5.1.B18 7.4
-
 assay at Spinacia oleracea
3.5.1.B18 7.4
-
 assay at Pisum sativum
3.5.1.B18 7.4
-
 assay at Zea mays
3.5.1.B18 7.4
-
 assay at Solanum lycopersicum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.B18 6.5 8.3 50% of maximal activity at pH 6.5 and pH 8.3, pH profile Arabidopsis thaliana

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.5.1.B18 0.052
-
 pH 7.4, 37°C, versus 4-aminobenzoylglutamate Arabidopsis thaliana folic acid